Anti-PA28 gamma PSME3 Monoclonal Antibody

Boster Bio Anti-PA28 gamma PSME3 Monoclonal Antibody catalog # A04375. Tested in WB applications. This antibody reacts with Human, Mouse, Pig.

Product Info Summary

SKU: A04375
Size: 100μl
Reactive Species: Human, Mouse, Pig
Host: Mouse
Application: WB

Product Name

Anti-PA28 gamma PSME3 Monoclonal Antibody

See all PA28 Activator gamma Subunit/PSME3 products

SKU/Catalog Number

A04375

Size

100μl

Form

Liquid

Description

Boster Bio Anti-PA28 gamma PSME3 Monoclonal Antibody catalog # A04375. Tested in WB applications. This antibody reacts with Human, Mouse, Pig.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PA28 gamma PSME3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04375)

Host

Mouse

Contents

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Reactive Species

A04375 is reactive to PSME3 in Human, Mouse, Pig

Applications

A04375 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:1000-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For PSME3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P61289

Gene ID

10197

Gene Name

PSME3

Full Name

Proteasome activator complex subunit 3

Weight

29.506kDa

Superfamily

PA28 family

Alternative Names

11S regulator complex gamma subunit; 11S regulator complex subunit gamma; Activator of multicatalytic protease subunit 3; Ki antigen; Ki; PA28 Activator gamma Subunit; PA28g; PA28gamma; PA28-gamma; PA28GKi nuclear autoantigen; proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki); Proteasome activator 28 subunit gamma; proteasome activator 28-gamma; proteasome activator complex subunit 3; PSME3; REG gamma; REG-GAMMA PSME3 HEL-S-283, Ki, PA28-gamma, PA28G, PA28gamma, REG-GAMMA proteasome activator subunit 3 proteasome activator complex subunit 3|11S regulator complex gamma subunit|11S regulator complex subunit gamma|Ki antigen|Ki nuclear autoantigen|PA28 gamma variant 5|REG gamma-3|activator of multicatalytic protease subunit 3|epididymis secretory protein Li 283|proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)|proteasome activator 28 subunit gamma|proteasome activator 28-gamma

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PSME3, check out the PSME3 Infographic

PSME3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PSME3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A04375

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-PA28 gamma PSME3 Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-PA28 gamma PSME3 Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

12 Customer Q&As for Anti-PA28 gamma PSME3 Monoclonal Antibody

Question

Would anti-PA28 gamma Monoclonal antibody A04375 work on pig WB with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

Our lab technicians have not validated anti-PA28 gamma Monoclonal antibody A04375 on pig. You can run a BLAST between pig and the immunogen sequence of anti-PA28 gamma Monoclonal antibody A04375 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-29

Question

Is a blocking peptide available for product anti-PA28 gamma Monoclonal antibody (A04375)?

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

We do provide the blocking peptide for product anti-PA28 gamma Monoclonal antibody (A04375). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-28

Question

Can you help my question with product A04375, anti-PA28 gamma Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-23

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04375 anti-PA28 gamma Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-23

Question

I see that the anti-PA28 gamma Monoclonal antibody A04375 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-08

Question

I was wanting to use to test anti-PA28 gamma Monoclonal antibody A04375 on pig embryonic kidney for research purposes, then I may be interested in using anti-PA28 gamma Monoclonal antibody A04375 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-08-02

Answer

The products we sell, including anti-PA28 gamma Monoclonal antibody A04375, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-02

Question

I was wanting to use your anti-PA28 gamma Monoclonal antibody for WB for pig embryonic kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for pig embryonic kidney identification?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

As indicated on the product datasheet, A04375 anti-PA28 gamma Monoclonal antibody has been tested for WB on human, mouse, pig tissues. We have an innovator award program that if you test this antibody and show it works in pig embryonic kidney in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-03

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-PA28 gamma Monoclonal antibody A04375. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-05-15

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-15

Question

Would A04375 anti-PA28 gamma Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-03-25

Answer

It shows on the product datasheet, A04375 anti-PA28 gamma Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-03-25

Question

Is there a BSA free version of anti-PA28 gamma Monoclonal antibody A04375 available?

Verified Customer

Verified customer

Asked: 2018-10-10

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PA28 gamma Monoclonal antibody A04375 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-10-10

Question

See attached the WB image, lot number and protocol we used for embryonic kidney using anti-PA28 gamma Monoclonal antibody A04375. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-06-29

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-06-29

Question

We are currently using anti-PA28 gamma Monoclonal antibody A04375 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, pig. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2017-10-25

Answer

The anti-PA28 gamma Monoclonal antibody (A04375) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-25

Question

Will anti-PA28 gamma Monoclonal antibody A04375 work for WB with embryonic kidney?

K. Li

Verified customer

Asked: 2015-02-26

Answer

According to the expression profile of embryonic kidney, PSME3 is highly expressed in embryonic kidney. So, it is likely that anti-PA28 gamma Monoclonal antibody A04375 will work for WB with embryonic kidney.

Boster Scientific Support

Answered: 2015-02-26

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A04375
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.