Human Survivin ELISA Kit PicoKine™

Human Survivin ELISA Kit PicoKine™ (96 Tests). Quantitate Human BIRC5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0641
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human Survivin ELISA Kit PicoKine™

See all Survivin products

SKU/Catalog Number

EK0641

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Survivin ELISA Kit PicoKine™ (96 Tests). Quantitate Human BIRC5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Survivin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0641)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-D142

Sensitivity

<2 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0641 is reactive to BIRC5 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0641 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0641-CAP Anti-Human BIRC5 Pre-coated 96-well strip microplate 1
EK0641-ST Human BIRC5 Standard 2 vials, 4 ng/tube
EK0641-DA Human BIRC5 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0500.1220.1780.2450.4310.7331.2751.902

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Survivin, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of Survivin in samples: 25248821 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Survivin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)20766012591926901195
Standard deviation15.5248.1851.6114.1357.2766.92
CV (%)7.5%7.3%4.1%8.3%8.3%5.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Survivin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 120717818518418811.015.8%
Sample 266072371274370930.664.3%
Sample 31259118710791117116068.85.9%
*number of samples for each test n=16.

Gene/Protein Information For BIRC5 (Source: Uniprot.Org, NCBI)

Uniprot ID

O15392

Gene ID

332

Gene Name

BIRC5

Full Name

Baculoviral IAP repeat-containing protein 5

Weight

16.389kDa

Superfamily

IAP family

Alternative Names

API4; apoptosis inhibitor 4; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; Baculoviral IAP Repeat-Containing Protein 5; BIRC5; EPR-1; IAP4; survivin variant 3 alpha; Survivin BIRC5 API4, EPR-1 baculoviral IAP repeat containing 5 baculoviral IAP repeat-containing protein 5|apoptosis inhibitor 4|apoptosis inhibitor survivin|survivin variant 3 alpha

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BIRC5, check out the BIRC5 Infographic

BIRC5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BIRC5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0641 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Serum Survivin Levels and Outcome of Chemotherapy in Patients with Malignant Mesothelioma

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13 Customer Q&As for Human Survivin ELISA Kit PicoKine™

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-09-03

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-09-03

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-05-26

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-05-26

Question

Has EK0641 kit been tested on exosomes samples?

Verified customer

Asked: 2019-09-25

Answer

The Human Survivin ELISA Kit PicoKine EK0641 has not been tested on exosomes, so there is no guarantee that this kit will work for those samples.

Boster Scientific Support

Answered: 2019-09-25

Question

Q: can you suggest the dilution ratio of serum samples for detection of Survivin in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2019-08-27

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Survivin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-08-27

Question

Do you have any suggestions for using EK0641 on urine samples? neat vs. diluted, etc.?

Verified customer

Asked: 2019-06-06

Answer

Our lab technicains haven't worked on urine samples. Please dilute the sample at 1:1. Optimal dilution ratio should be decided by the customer after performing serial dilutions.

Boster Scientific Support

Answered: 2019-06-10

Question

Do you have any commercial PBS buffer that could be used for EK0641 kit?

Verified customer

Asked: 2019-05-15

Answer

Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. You can still use wash buffers if you wish. Most commercial PBS buffers would work for our kit (TBS can be used as well). You can compare the formulation of the commercial buffer to our buffer preparation: Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Boster Scientific Support

Answered: 2019-05-15

Question

Q: how to proceed with the analysis of ELISA data? I have obtained Survivin level in plasma.

Verified Customer

Verified customer

Asked: 2019-02-10

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-02-10

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Survivin using the EK0641 Human Survivin Picokine® ELISA Kit?

L. Hernandez

Verified customer

Asked: 2018-12-07

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2018-12-07

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

J. Kapoor

Verified customer

Asked: 2018-09-29

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-09-29

Question

Q: how can I thaw whole blood sample for Survivin ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-09-27

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Survivin for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-09-27

Question

Q: Can Survivin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

J. Brown

Verified customer

Asked: 2018-05-17

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-05-17

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-01-28

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-01-28

Question

Q: can I use citrate plasma as samples in Human Survivin Picokine® ELISA Kit (Catalog # EK0641)?

Verified Customer

Verified customer

Asked: 2014-01-18

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Survivin causing degradation of its protein structure. Survivin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Survivin ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2014-01-18

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