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- Table of Contents
AQP1 antibody (M00865) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of AQP1 using anti-AQP1 antibody (M00865).
AQP1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-AQP1 Antibody (M00865) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
AQP1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-AQP1 Antibody (M00865) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-AQP1 Rabbit Monoclonal Antibody
AQP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
AQP1 antibody was positively detected by WB in:
rat kidney tissue, rat lung tissue, mouse kidney tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
AQP1 antibody was positively detected by IHC in:
human kidney tissue, mouse lung tissue, rat lung tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Human
Tested
Recommend
Recommend
Product Information
Product Name
Anti-AQP1 Rabbit Monoclonal Antibody
View all AQP1 Antibodies
View all AQP1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAEE-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AQP1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
25 kDa
Calculated Molecular Weight
28.5 kDa
Cite This Product
Anti-AQP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00865)
Protocols
Western Blot protocol for AQP1 antibody
Immunohistochemistry (IHC) protocol for AQP1 antibody
Publications
Application:
Species:
Dynamic expression and roles of sequestome‑1/p62 in LPS‑induced acute kidney injury in mice
Published: 2018-03-28
Journal: Molecular Medicine Reports
Application: IHC
Impact Factor: 1.92
Species: Mouse
PMID: 29620262
Effect of selective inhibition of aquaporin 1 on chemotherapy sensitivity of J82 human bladder cancer cells
Published: 2018-01-04
Journal: Oncology Letters
Application: WB
Impact Factor: 1.66
Species: Human
PMID: 29467903
Review
Have you used Anti-AQP1 Rabbit Monoclonal Antibody?
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1 Review For Anti-AQP1 Rabbit Monoclonal Antibody
In this Western blot experiment using Anti-AQP1 antibody (Cat# BM5035) on MADB106 rat mammary carcinoma cells, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.
| SKU | M00865 |
|---|---|
| Application | Western blot |
| Sample | MADB106 rat mammary carcinoma cells |
| Sample Processing Description | MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor |
| Other Reagents | RIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer |
| Primary Antibody | AQP1 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:3000, overnight at 4 ℃ |
| Secondary Antibody | 1:10,000, HRP-conjugated Goat Anti-Rabbit IgG |
| Secondary Incubation | 1h at RT |
| Detection | Substrate: ECL substrate, Imaging system:ChemiDoc MP |
| Results Summary | AQP1 (Aquaporin 1) is the first discovered water channel protein, whose primary function is efficient water transport. Recent studies have revealed that AQP1 plays a critical role in cancer progression, promoting tumor growth in breast cancer by enhancing angiogenesis and cell migration. In this experiment, AQP1 protein levels were markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor. |
Xinshuo Wang, Xi’an Jiaotong University
Verified customer
Submitted 2026-02-25
Product Promise
We promise Anti-AQP1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00865
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026