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AQP3 antibody (PA1488) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 13 Publications
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Flow Cytometry analysis of RT4 cells using anti-AQP3 antibody (PA1488).
Overlay histogram showing RT4 cells stained with PA1488 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-AQP3 Antibody (PA1488, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing RT4 cells stained with PA1488 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-AQP3 Antibody (PA1488, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Aquaporin 3/AQP3 Antibody Picoband®
AQP3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
AQP3 antibody was positively detected by WB in:
human Hela whole cell, rat kidney tissue, mouse kidney tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
AQP3 antibody was positively detected by IHC in:
mouse kidney tissue, rat kidney tissue, human bladder cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
AQP3 antibody was positively detected by ICC/IF in:
A431 cell
AQP3 antibody was positively detected by FC in:
RT4 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Aquaporin 3/AQP3 Antibody Picoband®
View all AQP3 Antibodies
View all AQP3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 3, different from the related rat and mouse sequences by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
61 kDa
Calculated Molecular Weight
31.5 kDa
Background of AQP3
This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Cite This Product
Anti-Aquaporin 3/AQP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1488)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
93%
High Homology
Rabbit
93%
High Homology
Dog
93%
High Homology
Monkey
93%
High Homology
Rat
87%
Moderate Homology
Cow
87%
Moderate Homology
Sheep
80%
Moderate Homology
Zebrafish
40%
Low homology
Drosophila
13%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for AQP3 antibody
Immunohistochemistry (IHC) protocol for AQP3 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for AQP3 antibody
Flow Cytometry (FC) protocol for AQP3 antibody
Publications
Application:
Species:
Sodium tanshinone IIA sulfonate alleviates fetal growth restriction by mediating aquaporin-3 expression in placental trophoblast cells
Published: 2025-01-18
Journal: FASEB JOURNAL
Application: WB,IHC
Impact Factor: 4.20
Species: Human,Mouse
PMID: 39825721
Activation of ASIC3/ERK pathway by paeoniflorin improves intestinal fluid metabolism and visceral sensitivity in slow transit constipated rats
Published: 2024-04-17
Journal: KAOHSIUNG JOURNAL OF MEDICAL SCIENCES
Application: WB
Impact Factor: 2.70
Species: Rat
PMID: 38634140
Stool-softening effect and action mechanism of free anthraquinones extracted from Rheum palmatum L. on water deficit-induced constipation in rats
Published: 2023-10-30
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IHC,WB
Impact Factor: 5.40
Species: Rat
PMID: 37907143
Tanshinone IIA changed the amniotic fluid volume and regulated expression of AQP1 and AQP3 in amniotic epithelium cells: a promising drug treating abnormal amniotic fluid volume
Published: 2023-06-29
Journal: MOLECULAR MEDICINE
Application: IHC
Impact Factor: 5.70
Species: Human
PMID: 37386378
Cerium Oxide Nanoparticles Regulate Oxidative Stress in HeLa Cells by Increasing the Aquaporin-Mediated Hydrogen Peroxide Permeability
Published: 2022-09-16
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 6.21
Species: Human
PMID: 36142747
Tanshinone IIA increased amniotic fluid volume through down-regulating placental AQPs expression via inhibiting the activity of GSK-3β
Published: 2022-06-08
Journal: CELL AND TISSUE RESEARCH
Application: ICC, WB
Impact Factor: 4.05
Species: Mouse, Human
PMID: 35674921
In vivo preclinical PET/CT imaging of carbon-11-labeled aminoglycerol probe for the diagnosis of liver fibrosis
Published: 2019-08-06
Journal: ANNALS OF NUCLEAR MEDICINE
Application: IHC
Impact Factor: 1.65
Species: Rat
PMID: 31388833
Aquaporin1–3 expression in normal and hydronephrotic kidneys in the human fetus
Published: 2019-07-01
Journal: PEDIATRIC RESEARCH
Application: IHC
Impact Factor: 2.88
Species: Human
PMID: 31261369
Lagopsis supina exerts its diuretic effect via inhibition of aquaporin-1, 2 and 3 expression in a rat model of traumatic blood stasis
Published: 2018-10-28
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IHC,WB
Impact Factor: 3.12
Species: Rat
PMID: 30394291
Agerarin, identified from Ageratum houstonianum, stimulates circadian CLOCK-mediated aquaporin-3 gene expression in HaCaT keratinocytes
Published: 2017-09-11
Journal: Scientific Reports
Application: FC
Impact Factor: 4.26
Species: Human
PMID: 28894278
Retinoic acid receptor-related orphan receptor RORα regulates differentiation and survival of keratinocytes during hypoxia
Published: 2017-03-23
Journal: JOURNAL OF CELLULAR PHYSIOLOGY
Application: WB
Impact Factor: 4.08
Species: Human
PMID: 28332183
RNA interference influenced the proliferation and invasion of XWLC-05 lung cancer cells through inhibiting aquaporin 3
Published: 2017-02-09
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Application: WB,IHC
Impact Factor: 2.47
Species: Mouse
PMID: 28189680
Expression pattern of aquaporins in patients with primary nephrotic syndrome with edema
Published: 2015-08-11
Journal: Molecular Medicine Reports
Application: IHC
Impact Factor: 1.55
Species: Human
PMID: 26261083
Review
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Submit A Review1 Review For Anti-Aquaporin 3/AQP3 Antibody Picoband®
This antibody is highly specific and efficient, suitable for Western blot detection of AQP3 protein in rat colon, with only minor nonspecific bands.
| SKU | PA1488 |
|---|---|
| Application | Western Blot |
| Sample | rat colon tissue |
| Sample Processing Description | RIPA lysis buffer: cells/tissues were lysed with PMSF protease inhibitor (100:1) for 10 min, followed by centrifugation at 12,000 rpm for 15 min. The supernatant was collected, mixed with 5× loading buffer, denatured at 100 °C for 10 min, and then loaded onto SDS-PAGE. |
| Other Reagents | Blocking buffer |
| Primary Antibody | Aquaporin 3/AQP3 Antibody Picoband® |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1:5000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | The figure shows representative Western blot results of the target protein AQP3 and the internal control Actin in rat colon across different groups. AQP3 expression was decreased in the model group and increased in all treatment groups, with the Western medicine group （PC）showing higher levels than the traditional Chinese medicine group (TCM). The target bands are clear and distinct, and the experimental results are satisfactory. |
Shiyu Zhang, LUTCM
Verified customer
Submitted 2025-12-29
Product Promise
We promise Anti-Aquaporin 3/AQP3 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1488
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 30, 2026