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- Table of Contents
AQP1 antibody (PB9473) performs well in Flow Cytometry, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Flow Cytometry analysis of U2OS cells using anti-AQP1 antibody (PB9473).
Overlay histogram showing U2OS cells stained with PB9473 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-AQP1 Antibody (PB9473, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U2OS cells stained with PB9473 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-AQP1 Antibody (PB9473, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Aquaporin 1/AQP1 Antibody Picoband®
AQP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, WB |
Tested Applications
AQP1 antibody was positively detected by WB in:
rat heart tissue, rat kidney tissue, rat lung tissue, mouse heart tissue, mouse kidney tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
AQP1 antibody was positively detected by IHC in:
human kidney tissue, mouse kidney tissue, rat kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
AQP1 antibody was positively detected by IF in:
rat kidney tissue
AQP1 antibody was positively detected by FC in:
U2OS cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Immunofluorescence
5μg/ml
Rat
Flow Cytometry(Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Tested
Mouse
Tested
Tested
Recommend
Human
Tested
Recommend
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Aquaporin 1/AQP1 Antibody Picoband®
View all AQP1 Antibodies
View all AQP1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 1, different from the related mouse and rat sequences by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
25, 35-38 kDa
Calculated Molecular Weight
28.5 kDa
Background of AQP1
Aquaporin 1 is a 28-kD integral protein thought at first to be a breakdown product of the Rh polypeptide but was later shown to be a unique molecule that is abundant in erythrocytes and renal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Cite This Product
Anti-Aquaporin 1/AQP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9473)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Mouse
97%
High Homology
Rat
97%
High Homology
Rabbit
93%
High Homology
Dog
93%
High Homology
Zebrafish
37%
Low homology
Drosophila
27%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for AQP1 antibody
Immunohistochemistry (IHC) protocol for AQP1 antibody
Immunofluorescence (IF) protocol for AQP1 antibody
Flow Cytometry (FC) protocol for AQP1 antibody
Publications
Application:
Species:
Lentivirus-mediated RNA interference targeting HMGB1 modulates AQP1 to reduce pain induced by chronic compression of the dorsal root ganglia
Published: 2024-09-18
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 4.40
Species: Rat
PMID: 39359252
Expression of AQP1, AQP3, AQP4 and AQP5 in upper respiratory tract of buffaloes during different seasons
Published: 2021-11-25
Journal: BIOLOGICAL RHYTHM RESEARCH
Application: IHC
Impact Factor: 1.22
Species: Bovine
Lead Exposure in Developmental Ages Promotes Aβ Accumulation by Disturbing Aβ Transportation in Blood-Cerebrospinal Fluid Barrier/Blood–Brain Barriers and Impairing Aβ Clearance in the Liver
Published: 2021-11-17
Journal: BIOLOGICAL TRACE ELEMENT RESEARCH
Application: IHC
Impact Factor: 3.74
Species: Rat
PMID: 34787833
Seasonal effect in expression of AQP1, AQP3 and AQP5 in skin of Murrah buffaloes
Published: 2020-09-11
Journal: JOURNAL OF THERMAL BIOLOGY
Application: IHC
Impact Factor: 2.36
Species: Buffalo
PMID: 33077138
Quantitative proteomics reveals TMOD1-related proteins associated with water balance regulation
Published: 2019-07-24
Journal: PLoS One
Application: IHC
Impact Factor: 2.78
Species: Mouse
PMID: 31339916
Lagopsis supina exerts its diuretic effect via inhibition of aquaporin-1, 2 and 3 expression in a rat model of traumatic blood stasis
Published: 2018-10-28
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IHC,WB
Impact Factor: 3.12
Species: Rat
PMID: 30394291
Early hyperbaric oxygen therapy inhibits aquaporin 4 and adrenocorticotropic hormone expression in the pituitary gland of rabbits with blast-induced craniocerebral injury
Published: 2012-08-08
Journal: Neural Regeneration Research
Application: IHC
Impact Factor: 0.22
Species: Rabbit
PMID: 25624795
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Product Promise
We promise Anti-Aquaporin 1/AQP1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9473
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026