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Product Info Summary
| SKU: | M00548-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-AREB6 (ZEB1) Mouse Monoclonal Antibody [Clone ID: OTI3G6]
SKU/Catalog Number
M00548-1
Size
100 µl
Description
Boster Bio ZEB1 mouse monoclonal antibody,clone OTI3G6. Catalog# M00548-1. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-AREB6 (ZEB1) Mouse Monoclonal Antibody [Clone ID: OTI3G6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00548-1)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI3G6
Isotype
IgG2a
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-350 of human ZEB1 (NP_110378) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00548-1 is reactive to ZEB1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
124.074kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00548-1 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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Cross-reactivity test with ZEB2: HEK293T cells were transfected with plasmids overexpressing either ZEB2 (left) or ZEB1 (right) and immunoblotted with anti-ZEB1 (M00548-1).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ZEB1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
Protein Target Info & Infographic
Gene/Protein Information For ZEB1 (Source: Uniprot.org, NCBI)
Gene Name
ZEB1
Full Name
Zinc finger E-box-binding homeobox 1
Weight
124.074kDa
Superfamily
delta-EF1/ZFH-1 C2H2-type zinc-finger family
Alternative Names
AREB6; AREB6MGC133261; BZP; delta-crystallin enhancer binding factor 1; DELTAEF1; FECD6; Negative regulator of IL2; NIL-2-A zinc finger protein; NIL2A; NIL-2-A; posterior polymorphous corneal dystrophy 3; PPCD3; TCF8; TCF-8; TCF8BZP; transcription factor 8 (represses interleukin 2 expression); Transcription factor 8; ZEB; ZEB1; ZFHEP; ZFHX1A; zinc finger E-box binding homeobox 1; zinc finger E-box-binding homeobox 1; zinc finger homeodomain enhancer-binding protein ZEB1 AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A zinc finger E-box binding homeobox 1 zinc finger E-box-binding homeobox 1|delta-crystallin enhancer binding factor 1|negative regulator of IL2|posterior polymorphous corneal dystrophy 3|transcription factor 8 (represses interleukin 2 expression)|zinc finger homeodomain enhancer-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZEB1, check out the ZEB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZEB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AREB6 (ZEB1) Mouse Monoclonal Antibody [Clone ID: OTI3G6] (M00548-1)
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