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Product Info Summary
| SKU: | A00742-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Argininosuccinate Lyase/ASL Antibody Picoband®
SKU/Catalog Number
A00742-1
Size
100 μg/vial
Form
Lyophilized
Description
ASL (argininosuccinate lyase) is classically placed in the urea cycle/arginine biosynthesis pathway (putative here due to page access failure), and is commonly investigated in inborn-error and nitrogen-disposal biology. Assay context: validated for Western blot with Mouse/Rat reactivity; metabolic-pathway studies may be contextualized with mitochondrial oxidation markers such as ACADM/MCAD or mitochondrial transport factors such as ABCB10 (both putative).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Argininosuccinate Lyase/ASL Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00742-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Argininosuccinate Lyase/ASL, which shares 97.4% and 92.1% amino acid (aa) sequence identity with mouse and rat Argininosuccinate Lyase/ASL, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00742-1 is reactive to ASL in Mouse, Rat
Observed Molecular Weight
52 kDa
Calculated molecular weight
51.7 kDa
Background of ASL
ASL (argininosuccinatelyase, also known as argininosuccinase) is an enzyme that catalyzes the reversible breakdown of argininosuccinate (ASA) producing the amino acid arginine and dicarboxylic acid fumarate. Located in liver cytosol, ASL is the fourth enzyme of the urea cycle and involved in the biosynthesis of arginine in all species and the production of urea in ureotelic species. Mutations in ASL, resulting low activity of the enzyme, increase levels of urea in the body and result in various side effects. The ASL gene is located on chromosome 7 between the centromere (junction of the long and short arm) and the long (q) arm at position 11.2, from base pair 64,984,963 to base pair 65,002,090.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00742-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: rat liver tissue, rat kidney tissue, rat lung tissue, mouse liver tissue
Validation Images & Assay Conditions
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Western blot analysis of Argininosuccinate Lyase/ASL using anti-Argininosuccinate Lyase/ASL antibody (A00742-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Argininosuccinate Lyase/ASL antigen affinity purified polyclonal antibody (Catalog # A00742-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Argininosuccinate Lyase/ASL at approximately 52KD. The expected band size for Argininosuccinate Lyase/ASL is at 52KD.
Specific Publications For Anti-Argininosuccinate Lyase/ASL Antibody Picoband® (A00742-1)
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3 Customer Q&As for Anti-Argininosuccinate Lyase/ASL Antibody Picoband®
Question
I am looking for to test anti-Adenylosuccinate Lyase/ASL antibody A00742-1 on rat liver for research purposes, then I may be interested in using anti-Adenylosuccinate Lyase/ASL antibody A00742-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
The products we sell, including anti-Adenylosuccinate Lyase/ASL antibody A00742-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-25
Question
Would anti-Adenylosuccinate Lyase/ASL antibody A00742-1 work on goat IHC with liver?
A. Jha
Verified customer
Asked: 2018-10-12
Answer
Our lab technicians have not validated anti-Adenylosuccinate Lyase/ASL antibody A00742-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Adenylosuccinate Lyase/ASL antibody A00742-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-12
Question
We are currently using anti-Adenylosuccinate Lyase/ASL antibody A00742-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-13
Answer
The anti-Adenylosuccinate Lyase/ASL antibody (A00742-1) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-13
