Rat IL-17F/Interleukin-17F ELISA Kit PicoKine™

Rat IL-17F ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il17f in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK1136
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Rat IL-17F/Interleukin-17F ELISA Kit PicoKine™

See all IL-17F products

SKU/Catalog Number

EK1136

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat IL-17F ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il17f in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat IL-17F/Interleukin-17F ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1136)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A19-A153

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1136 is reactive to Il17f in Rat samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1136 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1136-CAP Anti-Rat Il17f Pre-coated 96-well strip microplate 1
EK1136-ST Rat Il17f Standard 2 vials, 10 ng/tube
EK1136-DA Rat Il17f Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0060.1140.2250.3240.5611.0331.4442.063

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat IL-17F/Interleukin-17F ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5718896655186917
Standard deviation3.310.1541.533.9612.4652.26
CV (%)5.8%5.4%4.3%7.2%6.7%5.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-17F in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 157565653551.52.7%
Sample 218819521919319811.966%
Sample 3966948102389395746.44.8%
*number of samples for each test n=16.

Gene/Protein Information For Il17f (Source: Uniprot.Org, NCBI)

Uniprot ID

Q5BJ95

Gene ID

301291

Gene Name

Il17f

Full Name

Interleukin-17F

Weight

17.712kDa

Superfamily

IL-17 family

Alternative Names

Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; IL24; IL-24; interleukin 17F; Interleukin-24; ML-1; ML1interleukin-24 Il17f|IL-17F|interleukin 17F|interleukin-17F

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il17f, check out the Il17f Infographic

Il17f infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il17f: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1136 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ming Tang,Xia Gao,Ting Geng,Xialin Chen,Jiajia Wang,Canjie Shen,Huifang Gao,Mengyu Qian,Zhenzhong Wang,Liang Cao,Wei Xiao,Metabolomics analysis of the therapeutic effects of Qiwei Tongbi oral liquid on rheumatoid arthritis in rats,Journal of Pharmaceutical and Biomedical Analysis, Volume 202,2021,114166,ISSN 0731-7085,https://doi.org/10.1016/j.jpba.2021.114166.
Species: Rat
EK1136 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Li B,Du P,Du Y,Zhao D,Cai Y,Yang Q,Guo Z.Luteolin alleviates inflammation and modulates gut microbiota in ulcerative colitis rats.Life Sci.2021 Jan 9;269:119008.doi:10.1016/j.lfs.2020.119008.Epub ahead of print.PMID:33434535.
Species: Rat
EK1136 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA

Have you used Rat IL-17F/Interleukin-17F ELISA Kit PicoKine™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Rat IL-17F/Interleukin-17F ELISA Kit PicoKine™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Rat IL-17F/Interleukin-17F ELISA Kit PicoKine™

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring IL-17F using the EK1136 Rat IL-17F Picokine® ELISA Kit?

D. Carter

Verified customer

Asked: 2020-01-11

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2020-01-11

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-09-23

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-09-23

Question

Q: is it okay to use citrate plasma as samples in Rat IL-17F Picokine® ELISA Kit (Catalog # EK1136)?

L. Varma

Verified customer

Asked: 2018-07-29

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IL-17F causing degradation of its protein structure. IL-17F may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the IL-17F ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-07-29

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2017-06-27

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-06-27

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2017-02-15

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2017-02-15

Question

Q: which procedure should I follow in order to thaw whole blood sample for IL-17F ELISA after freezing?

Verified Customer

Verified customer

Asked: 2016-11-17

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-17F for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2016-11-17

Question

Q: how to analyze ELISA data? I have performed IL-17F level in cell lysates.

Verified Customer

Verified customer

Asked: 2014-08-23

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2014-08-23

$369

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK1136
$369.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.