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Product Info Summary
| SKU: | M12463 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-ARHGAP25 Mouse Monoclonal Antibody [Clone ID: OTI12G4]
SKU/Catalog Number
M12463
Size
100 µl
Description
Boster Bio ARHGAP25 mouse monoclonal antibody, clone OTI12G4 (formerly 12G4). Catalog# M12463. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-ARHGAP25 Mouse Monoclonal Antibody [Clone ID: OTI12G4] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12463)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI12G4
Isotype
IgG1
Immunogen
Full length human recombinant protein of human ARHGAP25 (NP_055697) produced in HEK293T cell.
Reactive Species
M12463 is reactive to ARHGAP25 in Human, Mouse, Rat
Calculated molecular weight
73.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M12463 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~2000
IHC 1:150
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGAP25.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGAP25 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either ARHGAP25 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARHGAP25 antibody (M12463)
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Anti-ARHGAP25 mouse monoclonal antibody (M12463) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARHGAP25.
Specific Publications For Anti-ARHGAP25 Mouse Monoclonal Antibody [Clone ID: OTI12G4] (M12463)
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