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Product Info Summary
| SKU: | A05258 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-AFAP1 Antibody
SKU/Catalog Number
A05258
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-AFAP1 Antibody (Catalog # A05258). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
AFAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-AFAP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05258)
Host
Rabbit
Contents
AFAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
AFAP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human AFAP1. The immunogen is located within amino acids 30 - 80 of AFAP1.
Cross-reactivity
Monomer and homomultimer of AFAP1 are known to exist; AFAP1 antibody is predicted to not cross-react with other AFAP family members.
Reactive Species
A05258 is reactive to AFAP1 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
80.7 kDa
Background of AFAP1
The actin filament-associated protein AFAP1 (AFAP-110) is an actin cross-linking protein first identified as a substrate of the viral oncogene v-Src. AFAP1 has a fundamental role in actin cytoskeleton arrangement. It contains a carboxyterminal actin-binding domain that directly binds to F-actin and serves as an adaptor protein in the regulation of SRC and PKC signal transduction by several functional domains, including 2 pleckstrin homology (PH) domains, a Src homology 3-binding (SH3-binding) motif, and several SH2-binding motifs. It is overexpressed in prostate carcinoma and contributes to tumor growth by regulating cell-matrix adhesions and migration in the cancer cells. AFAP1 represent a possible therapeutic target for controlling tumorigenesis and metastasis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05258 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
AFAP1 antibody can be used for detection of AFAP1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of AFAP1 in Hela cell lysate with AFAP1 antibody at (A) 1 and (B) 2 μg/mL.
Specific Publications For Anti-AFAP1 Antibody (A05258)
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