Anti-AFAP1 Antibody

AFAP antibody

Boster Bio Anti-AFAP1 Antibody (Catalog # A05258). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A05258
Size: 0.1 mg
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-AFAP1 Antibody

View all AFAP Antibodies

SKU/Catalog Number

A05258

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-AFAP1 Antibody (Catalog # A05258). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

AFAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-AFAP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05258)

Host

Rabbit

Contents

AFAP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

AFAP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human AFAP1. The immunogen is located within amino acids 30 - 80 of AFAP1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Monomer and homomultimer of AFAP1 are known to exist; AFAP1 antibody is predicted to not cross-react with other AFAP family members.

Reactive Species

A05258 is reactive to AFAP1 in Human, Mouse

Applications

A05258 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

80.725kDa

Background of AFAP

The actin filament-associated protein AFAP1 (AFAP-110) is an actin cross-linking protein first identified as a substrate of the viral oncogene v-Src. AFAP1 has a fundamental role in actin cytoskeleton arrangement. It contains a carboxyterminal actin-binding domain that directly binds to F-actin and serves as an adaptor protein in the regulation of SRC and PKC signal transduction by several functional domains, including 2 pleckstrin homology (PH) domains, a Src homology 3-binding (SH3-binding) motif, and several SH2-binding motifs. It is overexpressed in prostate carcinoma and contributes to tumor growth by regulating cell-matrix adhesions and migration in the cancer cells. AFAP1 represent a possible therapeutic target for controlling tumorigenesis and metastasis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

AFAP1 antibody can be used for detection of AFAP1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For AFAP1 (Source: Uniprot.org, NCBI)

Gene Name

AFAP1

Full Name

Actin filament-associated protein 1

Weight

80.725kDa

Alternative Names

actin filament associated protein 1; AFAP; AFAP1; AFAP-110; FLJ56849 AFAP1 AFAP, AFAP-11010, AFAP1 actin filament associated protein 1 actin filament-associated protein 1|110 kDa actin filament-associated protein|actin filament-associated protein, 110 kDa

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AFAP1, check out the AFAP1 Infographic

AFAP1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AFAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Size

Total: $449

SKU:A05258

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Lead time for this item is typically 4-7 days

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A05258
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$449.00

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