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- Table of Contents
CYP19A1 antibody (A00071) performs well in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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Flow Cytometry analysis of U20S cells using anti-Aromatase antibody (A00071).
Overlay histogram showing U20S cells stained with A00071 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aromatase Antibody (A00071,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing U20S cells stained with A00071 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aromatase Antibody (A00071,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-Aromatase/CYP19A1 Antibody Picoband®
CYP19A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB |
Tested Applications
CYP19A1 antibody was positively detected by WB in:
human placenta tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CYP19A1 antibody was positively detected by IHC in:
human placenta tissue, mouse small intestine tissue, rat lung tissue, rat small intestine tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CYP19A1 antibody was positively detected by FC in:
U20S cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Immunocytochemistry
0.5-1μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
ICC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Aromatase/CYP19A1 Antibody Picoband®
View all CYP19A1 Antibodies
View all CYP19A1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CYP19A1 recombinant protein (Position: Y241-H503).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
58 kDa
Calculated Molecular Weight
57.9 kDa
Background of CYP19A1
CYP19A1, also called Aromatase, is an enzyme responsible for a key step in the biosynthesis of estrogens. It is a member of the cytochrome P450 superfamily, which are monooxygenases that catalyze many reactions involved in steroidogenesis. In particular, aromatase is responsible for the aromatization of androgens into estrogens. The CYP19 gene spans at least 70 kb of genomic DNA and contains 10 exons. By in situ hybridization, the ARO gene is mapped to 15q21.1. The aromatase enzyme can be found in many tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, and endometrium, as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer. It is an important factor in sexual development. Some bodybuilders taking steroids also take antiaromatase supplements to prevent excess testosterone conversion into estrogens, which can cause gynecomastia.
Cite This Product
Anti-Aromatase/CYP19A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00071)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Rabbit
88%
Moderate Homology
Cow
87%
Moderate Homology
Sheep
86%
Moderate Homology
Dog
85%
Moderate Homology
Mouse
78%
Moderate Homology
Rat
76%
Moderate Homology
Zebrafish
56%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CYP19A1 antibody
Immunohistochemistry (IHC) protocol for CYP19A1 antibody
Flow Cytometry (FC) protocol for CYP19A1 antibody
Publications
Application:
Species:
FOXL2 Knockdown Inhibits the Progression of Endometriosis
Published: 2025-01-08
Journal: AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
Application: IHC
Impact Factor: 2.40
Species: Rat
PMID: 39776079
METTL3 deficiency leads to ovarian insufficiency due to IL-1β overexpression in theca cells
Published: 2024-05-31
Journal: FREE RADICAL BIOLOGY AND MEDICINE
Application: IHC
Impact Factor: 7.10
Species: Mouse
PMID: 38825211
Ti3C2 nanosheet-induced autophagy derails ovarian functions
Published: 2024-05-12
Journal: JOURNAL OF NANOBIOTECHNOLOGY
Application: WB
Impact Factor: 10.60
Species: Mouse,Human
PMID: 38735936
Effects of sub-chronic exposure to microcystin-LR on the endocrine system of male rats
Published: 2023-09-09
Journal: SCIENCE OF THE TOTAL ENVIRONMENT
Application: WB
Impact Factor: 9.80
Species: Rat
PMID: 37690761
miR-21, miR-125b, and let-7b contribute to the involution of atretic follicles and corpus lutea in Tibetan sheep ovaries
Published: 2022-07-13
Journal: ANIMAL SCIENCE JOURNAL
Application: WB
Impact Factor: 1.97
Species: Sheep
PMID: 35822516
ETA-mediated anti-TNF-α therapy ameliorates the phenotype of PCOS model induced by letrozole
Published: 2019-06-06
Journal: PLoS One
Application: IF
Impact Factor: 2.78
Species: Human,Rat
PMID: 31170164
Decreased hypothalamic aromatization in female rats of true precocious puberty
Published: 2004-06-09
Journal: NEUROSCIENCE LETTERS
Application: WB
Impact Factor: 1.97
Species: Rat
PMID: 15265597
Electroacupuncture enhances extragonadal aromatization in ovariectomized rats
Published: 2004-04-27
Journal: Reproductive Biology and Endocrinology
Application: WB
Impact Factor: 0.00
Species: Rat
PMID: 15113414
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Product Promise
We promise Anti-Aromatase/CYP19A1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00071
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026