Anti-Aromatase/CYP19A1 Antibody Picoband™

Boster Bio Anti-Aromatase/CYP19A1 Antibody Picoband™ catalog # A00071. Tested in ELISA, Flow Cytometry, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 5 publication(s).

Product Info Summary

SKU: A00071
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IHC-P, IHC-F, ICC, WB

Product Name

Anti-Aromatase/CYP19A1 Antibody Picoband™

See all Aromatase products

SKU/Catalog Number

A00071

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Aromatase/CYP19A1 Antibody Picoband™ catalog # A00071. Tested in ELISA, Flow Cytometry, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Aromatase/CYP19A1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00071)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human CYP19A1 recombinant protein (Position: Y241-H503).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00071 is reactive to CYP19A1 in Human, Mouse, Rat

Applications

A00071 is guaranteed for ELISA, Flow Cytometry, IHC-P, IHC-F, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For CYP19A1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P11511

Gene ID

1588

Gene Name

CYP19A1

Full Name

Aromatase

Weight

57.883kDa

Superfamily

cytochrome P450 family

Alternative Names

ARO1EC 1.14.14.1; Aromatase; AROsubfamily XIX (aromatization of androgens); CYARMGC104309; CYPXIX; cytochrome P450, family 19, subfamily A, polypeptide 1; Cytochrome P-450AROM; Estrogen synthase; microsomal monooxygenase CYP19A1 ARO, ARO1, CPV1, CYAR, CYP19, CYPXIX, P-450AROM cytochrome P450 family 19 subfamily A member 1 aromatase|cytochrome P-450AROM|cytochrome P450 19A1|cytochrome P450, family 19, subfamily A, polypeptide 1|cytochrome P450, subfamily XIX (aromatization of androgens)|estrogen synthase|estrogen synthetase|flavoprotein-linked monooxygenase|microsomal monooxygenase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CYP19A1, check out the CYP19A1 Infographic

CYP19A1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CYP19A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00071 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Feng F,Wang J,Bao R,Li L,Tong X,Han S,Zhang H,Wen W,Xiao L,Zhang C.LncPrep + 96kb 2.2 kb Inhibits Estradiol Secretion From Granulosa Cells by Inducing EDF1 Translocation.Front Cell Dev Biol.2020 Jun 30;8:481.doi:10.3389/fcell.2020.00481.PMID:32695776;PMCI
Species: Mouse
A00071 usage in article: APP:WB, SAMPLE:GRANULOSA CELL, DILUTION:NA

Xuan Luo,Hui Liu,Hongzhou Guo et al.RAF1 as Downstream Molecule Mediates the FSH Signaling Pathway to Stimulate E2 Synthesis and Secretion in Mouse Ovarian Granulosa Cells, 22 September 2020, PREPRINT (Version 1) available at Research Square [https://doi.
Species: Mouse
A00071 usage in article: APP:WB, SAMPLE:OVARIES, DILUTION:1:2000

Electroacupuncture enhances extragonadal aromatization in ovariectomized rats

Extragonadal aromatization increases with time after ovariectomy in rats

Zhang D, Yang B, Zou W, Lu X, Xiong M, Wu L, Wang J, Gao J, Xu S, Zou T. Biomed Res Int. 2013;2013:301540. Doi: 10.1155/2013/301540. Epub 2012 Dec 26. Estradiol Synthesis And Release In Cultured Female Rat Bone Marrow Stem Cells.

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16 Customer Q&As for Anti-Aromatase/CYP19A1 Antibody Picoband™

Question

I am interested in using your anti-Aromatase/CYP19A1 antibody for androgen catabolic process studies. Has this antibody been tested with western blotting on u20s cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

Thanks for your inquiry. This A00071 anti-Aromatase/CYP19A1 antibody is validated on human placenta tissue, tissue lysate, u20s cells. It is guaranteed to work for ELISA, Flow Cytometry, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-04-22

Question

Is a blocking peptide available for product anti-Aromatase/CYP19A1 antibody (A00071)?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

We do provide the blocking peptide for product anti-Aromatase/CYP19A1 antibody (A00071). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-09

Question

I was wanting to use to test anti-Aromatase/CYP19A1 antibody A00071 on rat ovary placenta for research purposes, then I may be interested in using anti-Aromatase/CYP19A1 antibody A00071 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-04-02

Answer

The products we sell, including anti-Aromatase/CYP19A1 antibody A00071, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-04-02

Question

Do you have a BSA free version of anti-Aromatase/CYP19A1 antibody A00071 available?

Verified Customer

Verified customer

Asked: 2020-03-25

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Aromatase/CYP19A1 antibody A00071 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-25

Question

We have been able to see staining in human ovary placenta. Any tips? Is anti-Aromatase/CYP19A1 antibody supposed to stain ovary placenta positively?

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

According to literature ovary placenta does express CYP19A1. According to Uniprot.org, CYP19A1 is expressed in chorionic villus, placenta, ovary placenta, among other tissues. Regarding which tissues have CYP19A1 expression, here are a few articles citing expression in various tissues:
Ovary, and Placenta, Pubmed ID: 15489334
Placenta, Pubmed ID: 2541021, 2691883, 2973313, 3018730, 3964273

Boster Scientific Support

Answered: 2019-12-16

Question

I see that the anti-Aromatase/CYP19A1 antibody A00071 works with IHC-F, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-31

Answer

You can find protocols for IHC-F on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-31

Question

We purchased anti-Aromatase/CYP19A1 antibody for ICC on chorionic villus in the past. I am using human, and We are going to use the antibody for WB next. My lab would like examining chorionic villus as well as placenta in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-10-18

Answer

I looked at the website and datasheets of our anti-Aromatase/CYP19A1 antibody and it appears that A00071 has been validated on human in both ICC and WB. Thus A00071 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-10-18

Question

Does anti-Aromatase/CYP19A1 antibody A00071 work for IHC-F with ovary placenta?

L. Walker

Verified customer

Asked: 2018-12-31

Answer

According to the expression profile of ovary placenta, CYP19A1 is highly expressed in ovary placenta. So, it is likely that anti-Aromatase/CYP19A1 antibody A00071 will work for IHC-F with ovary placenta.

Boster Scientific Support

Answered: 2018-12-31

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovary placenta using anti-Aromatase/CYP19A1 antibody A00071. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-11-29

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-11-29

Question

We are currently using anti-Aromatase/CYP19A1 antibody A00071 for rat tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2017-10-24

Answer

The anti-Aromatase/CYP19A1 antibody (A00071) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-24

Question

Is this A00071 anti-Aromatase/CYP19A1 antibody reactive to the isotypes of CYP19A1?

Verified Customer

Verified customer

Asked: 2017-10-20

Answer

The immunogen of A00071 anti-Aromatase/CYP19A1 antibody is E. coli-derived human CYP19A1 recombinant protein (Position: Y241-H503). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-10-20

Question

I was wanting to use your anti-Aromatase/CYP19A1 antibody for IHC-F for rat ovary placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat ovary placenta identification?

M. Wu

Verified customer

Asked: 2016-12-26

Answer

As indicated on the product datasheet, A00071 anti-Aromatase/CYP19A1 antibody has been validated for ELISA, Flow Cytometry, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat ovary placenta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-12-26

Question

See attached the WB image, lot number and protocol we used for ovary placenta using anti-Aromatase/CYP19A1 antibody A00071. Please let me know if you require anything else.

G. Baker

Verified customer

Asked: 2016-08-22

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-08-22

Question

Does A00071 anti-Aromatase/CYP19A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

S. Carter

Verified customer

Asked: 2015-07-16

Answer

It shows on the product datasheet, A00071 anti-Aromatase/CYP19A1 antibody as been validated on IHC-F. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-07-16

Question

Can you help my question with product A00071, anti-Aromatase/CYP19A1 antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Zhao

Verified customer

Asked: 2014-03-13

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00071 anti-Aromatase/CYP19A1 antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-03-13

Question

My colleagues were content with the WB result of your anti-Aromatase/CYP19A1 antibody . However we have seen positive staining in ovary placenta endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is CYP19A1 supposed to be expressed?

B. Taylor

Verified customer

Asked: 2013-10-15

Answer

According to literature, ovary placenta does express CYP19A1. Generally CYP19A1 expresses in endoplasmic reticulum membrane. Regarding which tissues have CYP19A1 expression, here are a few articles citing expression in various tissues:
Ovary, and Placenta, Pubmed ID: 15489334
Placenta, Pubmed ID: 2541021, 2691883, 2973313, 3018730, 3964273

Boster Scientific Support

Answered: 2013-10-15

Conjugation

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A00071
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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