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Product Info Summary
| SKU: | PA2121 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Protein Wnt-4 Wnt4 Antibody Picoband®
SKU/Catalog Number
PA2121
BA3160-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Protein Wnt-4 Wnt4 Antibody catalog # PA2121. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein Wnt-4 Wnt4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2121)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Wnt4, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2121 is reactive to WNT4 in Human, Mouse, Rat
Observed Molecular Weight
37 kDa
Calculated molecular weight
39.1 kDa
Background of WNT4
Wingless-type MMIV integration site family, member4, is a secreted protein that in humans is encoded by the Wnt4 gene. By fluorescence in situ hybridization, WNT4 gene was mapped to chromosome 1p35, a location consistent with the results of radiation hybrid mapping. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and embryogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2121 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: MCF-7 Whole Cell
Validation Images & Assay Conditions
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Anti-WNT4 antibody, PA2121, Western blotting
All lanes: Anti WNT4 (PA2121) at 0.5ug/ml
WB: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 37KD
Observed bind size: 37KD
Specific Publications For Anti-Protein Wnt-4 Wnt4 Antibody Picoband® (PA2121)
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Customer Q&As
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4 Customer Q&As for Anti-Protein Wnt-4 Wnt4 Antibody Picoband®
Question
We are currently using anti-Wnt4 antibody PA2121 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
The anti-Wnt4 antibody (PA2121) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-28
Question
Is there a BSA free version of anti-Wnt4 antibody PA2121 available?
E. Li
Verified customer
Asked: 2019-10-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Wnt4 antibody PA2121 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-15
Question
Is a blocking peptide available for product anti-Wnt4 antibody (PA2121)?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
We do provide the blocking peptide for product anti-Wnt4 antibody (PA2121). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-08-14
Question
My question regarding product PA2121, anti-Wnt4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Jackson
Verified customer
Asked: 2016-04-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2121 anti-Wnt4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-04-13
