Anti-Acid-sensing ion channel 3 ASIC3 Antibody Picoband®

Product Name

Anti-Acid-sensing ion channel 3 ASIC3 Antibody Picoband®

View all ASIC3 Antibodies

SKU/Catalog Number

PA1938

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Acid-sensing ion channel 3 ASIC3 Antibody catalog # PA1938. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Acid-sensing ion channel 3 ASIC3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1938)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ASIC3, different from the related rat and mouse sequences by two amino acids.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1938 is reactive to ASIC3 in Human, Mouse, Rat

Observed Molecular Weight

59 kDa

Calculated molecular weight

58.9 kDa

Background of ASIC3

ASIC3 (Acid-Sensing Ion Channel3), also known as TESTIS SODIUM CHANNEL 1 (TNAC1) or DORSAL ROOT ACID-SENSING ION CHANNEL (DRASIC), is a protein that in humans is encoded by the ASIC3 gene. ASIC3 belongs to a family of acid-sensing channel proteins that are structurally related to epithelial sodium channel proteins and support acid-activated membrane currents. By radiation hybrid analysis, de Weille et al. (1998) mapped the ACCN3 gene to chromosome 7q35. De Weille et al. (1998) found that human ASIC3 supported an H (+)-gated cation current in COS cells with kinetics similar to those of rat Asic3. Babinski et al. (1999) expressed homomeric human ASIC3 channels in Xenopus oocytes and found that rapid reduction in extracellular pH resulted in a biphasic response characterized by a fast and rapidly desensitizing current followed by a slow and sustained current that returned to baseline only on return to physiologic pH.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1938 is guaranteed for Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Positive Control

WB: human SH-SY5Y whole cell, human U87 whole cell, human U251 whole cell, human HepG2 whole cell, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue

Validation Images & Assay Conditions

See full target information ASIC3

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