Anti-Acid-sensing ion channel 3 ASIC3 Antibody

Product Name

Anti-Acid-sensing ion channel 3 ASIC3 Antibody

View all Amiloride-sensitive cation channel 3 Antibodies

SKU/Catalog Number

PA1938

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Acid-sensing ion channel 3 ASIC3 Antibody catalog # PA1938. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Acid-sensing ion channel 3 ASIC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1938)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human ASIC3, different from the related rat and mouse sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1938 is reactive to ASIC3 in Human, Mouse, Rat

Applications

PA1938 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

69 kDa

Calculated molecular weight

58.905kDa

Background of Amiloride-sensitive cation channel 3

ASIC3 (Acid-Sensing Ion Channel3), also known as TESTIS SODIUM CHANNEL 1 (TNAC1) or DORSAL ROOT ACID-SENSING ION CHANNEL (DRASIC), is a protein that in humans is encoded by the ASIC3 gene. ASIC3 belongs to a family of acid-sensing channel proteins that are structurally related to epithelial sodium channel proteins and support acid-activated membrane currents. By radiation hybrid analysis, de Weille et al. (1998) mapped the ACCN3 gene to chromosome 7q35. De Weille et al. (1998) found that human ASIC3 supported an H (+)-gated cation current in COS cells with kinetics similar to those of rat Asic3. Babinski et al. (1999) expressed homomeric human ASIC3 channels in Xenopus oocytes and found that rapid reduction in extracellular pH resulted in a biphasic response characterized by a fast and rapidly desensitizing current followed by a slow and sustained current that returned to baseline only on return to physiologic pH.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of ASIC3 using anti-ASIC3 antibody (PA1938).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Brain Tissue Lysate,
Lane 2: Rat Testis Tissue Lysate,
Lane 3: U87 Cell Lysate,
Lane 4: NEURO Cell Lysate,
Lane 5: SMMC Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASIC3 antigen affinity purified polyclonal antibody (Catalog # PA1938) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASIC3 at approximately 69KD. The expected band size for ASIC3 is at 58KD.

Click image to see more details

Figure 2. Flow Cytometry analysis of U937 cells using anti-ASIC3 antibody (PA1938).
Overlay histogram showing U937 cells stained with PA1938 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASIC3 Antibody (PA1938,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For ASIC3 (Source: Uniprot.org, NCBI)

Alternative Names

Acid-sensing ion channel 3; amiloride-sensitive cation channel 3; amiloride-sensitive cation channel 3, testis; ASIC3hTNaC1; DRASIC; hASIC3; modulatory subunit of ASIC2a; Neuronal amiloride-sensitive cation channel 3; proton-gated cation channel subunit; SLNAC1; Testis sodium channel 1; TNaC1 ASIC3 ACCN3, DRASIC, SLNAC1, TNaC1 acid sensing ion channel subunit 3 acid-sensing ion channel 3|acid sensing (proton gated) ion channel 3|amiloride-sensitive cation channel 3, testis|modulatory subunit of ASIC2a|neuronal amiloride-sensitive cation channel 3|proton-gated cation channel subunit|testis sodium channel 1

For more info on ASIC3, check out the ASIC3 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ASIC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the ASIC3 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis