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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||Flow Cytometry, WB|
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Anti-Acid-sensing ion channel 3 ASIC3 Antibody
Boster Bio Anti-Acid-sensing ion channel 3 ASIC3 Antibody catalog # PA1938. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Acid-sensing ion channel 3 ASIC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1938)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the N-terminus of human ASIC3 (56-73aa FLYQVAERVRYYREFHHQ), different from the related rat and mouse sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross-reactivity with other proteins
PA1938 is reactive to ASIC3 in Human, Mouse, Rat
PA1938 is guaranteed for Flow Cytometry, WB Boster Guarantee
Background of Amiloride-sensitive cation channel 3
ASIC3 (Acid-Sensing Ion Channel3), also known as TESTIS SODIUM CHANNEL 1 (TNAC1) or DORSAL ROOT ACID-SENSING ION CHANNEL (DRASIC), is a protein that in humans is encoded by the ASIC3 gene. ASIC3 belongs to a family of acid-sensing channel proteins that are structurally related to epithelial sodium channel proteins and support acid-activated membrane currents. By radiation hybrid analysis, de Weille et al. (1998) mapped the ACCN3 gene to chromosome 7q35. De Weille et al. (1998) found that human ASIC3 supported an H (+)-gated cation current in COS cells with kinetics similar to those of rat Asic3. Babinski et al. (1999) expressed homomeric human ASIC3 channels in Xenopus oocytes and found that rapid reduction in extracellular pH resulted in a biphasic response characterized by a fast and rapidly desensitizing current followed by a slow and sustained current that returned to baseline only on return to physiologic pH.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ASIC3 using anti-ASIC3 antibody (PA1938).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Brain Tissue Lysate,
Lane 2: Rat Testis Tissue Lysate,
Lane 3: U87 Cell Lysate,
Lane 4: NEURO Cell Lysate,
Lane 5: SMMC Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASIC3 antigen affinity purified polyclonal antibody (Catalog # PA1938) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASIC3 at approximately 69KD. The expected band size for ASIC3 is at 58KD.
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Figure 2. Flow Cytometry analysis of U937 cells using anti-ASIC3 antibody (PA1938).
Overlay histogram showing U937 cells stained with PA1938 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASIC3 Antibody (PA1938,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Gene/Protein Information For ASIC3 (Source: Uniprot.org, NCBI)
Acid-sensing ion channel 3
amiloride-sensitive sodium channel (TC 1.A.6) family
Acid-sensing ion channel 3; amiloride-sensitive cation channel 3; amiloride-sensitive cation channel 3, testis; ASIC3hTNaC1; DRASIC; hASIC3; modulatory subunit of ASIC2a; Neuronal amiloride-sensitive cation channel 3; proton-gated cation channel subunit; SLNAC1; Testis sodium channel 1; TNaC1 ASIC3 ACCN3, DRASIC, SLNAC1, TNaC1 acid sensing ion channel subunit 3 acid-sensing ion channel 3|acid sensing (proton gated) ion channel 3|amiloride-sensitive cation channel 3, testis|modulatory subunit of ASIC2a|neuronal amiloride-sensitive cation channel 3|proton-gated cation channel subunit|testis sodium channel 1*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on ASIC3, check out the ASIC3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ASIC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
PA1938 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of knockout of the ASIC3 on cardiovascular reflexes arising from hindlimb muscle in decerebrated rats
Schuhmacher LN, Callejo G, Srivats S, Smith ESJ. J Biol Chem. 2018 Feb 2;293(5):1756-1766. doi: 10.1074/jbc.M117.807859. Epub 2017 Dec 13. Naked mole-rat acid-sensing ion channel 3 forms nonfunctional homomers, but functional heteromers
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