|Validated Species:||Human, Mouse, Rat|
|Application:||Flow Cytometry, IHC, ICC, WB|
Data & Images
|Product Name||Anti-ASIC3 Antibody|
|Description||Rabbit IgG polyclonal antibody for Acid-sensing ion channel 3(ASIC3) detection. Tested with WB, IHC-F, ICC, FCM in Human;Mouse;Rat.|
|Cite This Product||Anti-ASIC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1938)|
|Replacement Item||This antibody may replace the following items: sc-130965|sc-21845|sc-368068 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||Flow Cytometry, IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human ASIC3(56-73aa FLYQVAERVRYYREFHHQ), different from the related rat and mouse sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Acid-sensing ion channel 3|
|Molecular Weight||58905 MW|
|Protein Function||Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties. .|
|Tissue Specificity||Expressed by sensory neurons. Strongly expressed in brain, spinal chord, lung, lymph nodes, kidney, pituitary, heart and testis. .|
|Sequence Similarities||Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC3 subfamily.|
|Subcellular Localization||Cell membrane ; Multi-pass membrane protein . Cytoplasm . Cell surface expression may be stabilized by interaction with LIN7B and cytoplasmic retention by interaction with DLG4. In part cytoplasmic in cochlea cells (By similarity). .|
|Alternative Names||Acid-sensing ion channel 3;ASIC3;hASIC3;Amiloride-sensitive cation channel 3;Neuronal amiloride-sensitive cation channel 3;Testis sodium channel 1;hTNaC1;ASIC3;ACCN3, SLNAC1, TNAC1;|
|Research Areas|||neuroscience|neurotransmission|receptors / channels|sodium channels| neuroscience|sensory system|somatosensory system|nociception||
Background for Acid-sensing ion channel 3
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-ASIC3 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-ASIC3 Antibody Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Testis Tissue Lysate
Lane 3: U87 Cell Lysate
Lane 4: NEURO Cell Lysate
Lane 5: SMMC Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,