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Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse PTX3/Pentraxin 3. 96wells/kit, with removable strips.
Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0862 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Mouse |
| Sample Type: | cell culture supernates, serum and plasma(EDTA). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <10pg/ml |
| Assay Range: | 156pg/ml-10000pg/ml |
Product Features:
Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.
Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.
Overview
| Product Name | Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™
See all Pentraxin 3/TSG-14 primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0862 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Mouse Pentraxin 3/PTX3 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Pentraxin 3/PTX3 tissue specificity: |
| Cite This Product | Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0862) |
Product Specs
| Reactivity/Species | Mouse |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, serum and plasma(EDTA). |
| Sensitivity | <10pg/ml |
| Assay Range | 156pg/ml-10000pg/ml |
| Immunogen/Standard | Expression system for standard: NSO; Immunogen sequence: E18-S381 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Gene/Protein Basic Information For Ptx3 (Source: Uniprot.org, NCBI)
| Uniprot Id | P48759 |
|---|---|
| NCBI Gene Id | 19288 |
| Species Of This Entry | Mouse |
| Gene Name | Ptx3 |
| Protein Name | Pentraxin-related protein PTX3 |
| Alternative Names | Pentraxin 3/TSG-14|alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14 |
| Molecular Weight | 41812 |
| Post Tranlational Modifications | Cleavage; Glycosylation; Phosphorylation; Methylation; Demethylation; Citrullination; Acetylation; Deglycosylation |
| Gene Location | 3 E1|3 30.25 cM, on Chromasome 3, gene sequence: |
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Ptx3 (Source: Uniprot.org, NCBI)
| Protein Function | Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self- components and female fertility. |
|---|---|
| Related Diseases | Inflammation; Atherosclerosis; Inflammatory Response; Cardiovascular Diseases; Myocardial Infarction; Infarction; Infective Disorder; Tumor Angiogenesis; Autoimmune Reaction; Heart Failure; Hypertensive Disease; Acute Myocardial Infarction; Kidney Diseases; Fibrosis; Innate Immune Response; Angina Pectoris; Neoplasms; Pre-eclampsia; Dental Plaque; Kidney Failure, Chronic |
| Related Pathways | Pathogenesis; Inflammatory Response; Immune Response; Complement Activation; Angiogenesis; Phagocytosis; Secretion; Innate Immune Response; Localization; Fertilization; Cell Death; Acute-phase Response; Coagulation; Neurogenesis; Glomerular Filtration; Ovulation; Apoptotic Cell Clearance; Oocyte Maturation; Opsonization; Cell Proliferation |
| Background | PTX3(Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs. |
| Scientific References | PMID: 8634434 by Introna M., et al. Cloning of mouse ptx3, a new member of the pentraxin gene family expressed at extrahepatic sites. PMID: 7592730 by Altmeyer A., et al. Promoter structure and transcriptional activation of the murine TSG- 14 gene encoding a tumor necrosis factor/interleukin-1-inducible pentraxin protein. |
Kit Components And Assay QC Data Details Of Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0862-CAP | 96-well plate precoated with anti-Mouse Pentraxin 3/TSG-14 antibody | 1 |
| EK0862-ST | lyophilized recombinant Mouse Pentraxin 3/TSG-14 standard | 10ng/tube |
| EK0862-DA | biotinylated anti-Mouse Pentraxin 3/TSG-14 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0862 Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.035 | 0.119 | 0.209 | 0.344 | 0.626 | 1.028 | 1.542 | 2.207 |
We measured random samples of Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 262 | 1334 | 4146 | 264 | 1451 | 3917 |
| Standard deviation | 15.19 | 98.71 | 211.44 | 15.84 | 113.17 | 274.19 |
| CV(%) | 5.8% | 7.4% | 7% | 6% | 7.8% | 7% |
We measured the performance consistency of Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 262 | 259 | 249 | 272 | 260 | 8.2 | 3.1% |
| Sample 2 | 1334 | 1350 | 1444 | 1511 | 1409 | 71.99 | 5.1% |
| Sample 3 | 4146 | 4596 | 4000 | 4523 | 4316 | 250 | 5.7% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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| (888) 466-3604 |
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