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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (EDTA).|
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Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ptx3 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0862)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: E18-S381
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0862 is reactive to Ptx3 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA).
EK0862 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0862-CAP||Anti-Mouse Ptx3 Pre-coated 96-well strip microplate||1|
|EK0862-ST||Mouse Ptx3 Standard||2 vials, 10 ng/tube|
|EK0862-DA||Mouse Ptx3 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse PTX3/Pentraxin 3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Pentraxin 3/TSG-14, Dilution ratio of 1:20, concentration in serum is around 36ng/ml.
Some articles we found to cite concentrations of Pentraxin 3/TSG-14 in samples: 22732935 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Pentraxin 3/TSG-14 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Ptx3 (Source: Uniprot.Org, NCBI)
Pentraxin-related protein PTX3
alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14 Ptx3|AI607804, TSG-1, TSG-14|pentraxin related gene|pentraxin-related protein PTX3|pentaxin related|pentaxin-related protein PTX3|pentraxin 3|tumor necrosis factor-inducible gene 14 protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Ptx3, check out the Ptx3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ptx3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK0862
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