Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™

Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ptx3 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0862
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (EDTA).

Product Name

Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™

See all Pentraxin 3/TSG-14 products

SKU/Catalog Number

EK0862

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ptx3 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0862)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E18-S381

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0862 is reactive to Ptx3 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (EDTA).

Application Guarantee

EK0862 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0862-CAP Anti-Mouse Ptx3 Pre-coated 96-well strip microplate 1
EK0862-ST Mouse Ptx3 Standard 2 vials, 10 ng/tube
EK0862-DA Mouse Ptx3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0350.1190.2090.3440.6261.0281.5422.207

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Pentraxin 3/TSG-14, Dilution ratio of 1:20, concentration in serum is around 36ng/ml.

Some articles we found to cite concentrations of Pentraxin 3/TSG-14 in samples: 22732935 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2621334414626414513917
Standard deviation15.1998.71211.4415.84113.17274.19
CV (%)5.8%7.4%7%6%7.8%7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Pentraxin 3/TSG-14 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12622592492722608.23.1%
Sample 21334135014441511140971.995.1%
Sample 3414645964000452343162505.7%
*number of samples for each test n=16.

Gene/Protein Information For Ptx3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P48759

Gene ID

19288

Gene Name

Ptx3

Full Name

Pentraxin-related protein PTX3

Weight

41.812kDa

Alternative Names

alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14 Ptx3|AI607804, TSG-1, TSG-14|pentraxin related gene|pentraxin-related protein PTX3|pentaxin related|pentaxin-related protein PTX3|pentraxin 3|tumor necrosis factor-inducible gene 14 protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ptx3, check out the Ptx3 Infographic

Ptx3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ptx3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK0862

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-11-09

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-11-09

Question

Q: is it okay to use heparin plasma as samples in Mouse Pentraxin 3 Picokine® ELISA Kit (Catalog # EK0862)?

Verified Customer

Verified customer

Asked: 2020-06-30

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Pentraxin 3 causing disruption of its protein structure. Pentraxin 3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Pentraxin 3 ELISA, treating samples with a number of anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-06-30

Question

Q: how to thaw whole blood sample for Pentraxin 3 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-07-17

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Pentraxin 3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-07-17

Question

Q: if the enzyme conjugated Pentraxin 3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Pentraxin 3 antigen?

B. Gonzalez

Verified customer

Asked: 2019-06-11

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-06-11

Question

Q: how to proceed with the analysis of ELISA data? I have obtained Pentraxin 3 level in serum.

Verified Customer

Verified customer

Asked: 2018-09-14

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-09-14

Question

Q: What is the optimal O.D. value for Pentraxin 3 ELISA kit? I performed your Pentraxin 3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Pentraxin 3 even though the O.D. values are not very high?

A. Thompson

Verified customer

Asked: 2018-04-16

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Pentraxin 3 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Pentraxin 3 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-04-16

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2017-06-06

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-06-06

$369

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK0862
$369.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.