Overview
| Product Name |
Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™
See more Pentraxin 3/TSG-14 products |
| Catalog# |
EK0862 |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ptx3 in cell culture supernatants, serum and plasma (EDTA).. Sensitivity: 10pg/ml. |
| Cite This Product |
Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0862)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Mouse |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, serum and plasma (EDTA). |
| Sensitivity |
<10 pg/ml |
| Assay Range |
156 pg/ml - 10,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: E18-S381 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For Ptx3 (Source: Uniprot.org, NCBI)
| Uniprot Id | P48759 |
|---|
| NCBI Gene Id | 19288 |
|---|
| Species Of This Entry | Mouse |
|---|
| Gene Name | Ptx3 |
|---|
| Protein Name | Pentraxin-related protein PTX3 |
|---|
| Alternative Names | Pentraxin 3/TSG-14|alpha-induced protein 5; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor; Pentaxin-related protein PTX3; Pentraxin 3; pentraxin 3, long; pentraxin-3; pentraxin-related gene, rapidly induced by IL-1 beta; pentraxin-related protein PTX3; PTX3; TNF alpha-induced protein 5; TNFAIP5; TSG14; TSG-14; TSG-14pentaxin-related gene, rapidly induced by IL-1 beta; Tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; Tumor necrosis factor-inducible gene 14 protein; tumor necrosis factor-inducible protein TSG-14 |
|---|
| Molecular Weight | 41812 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Ptx3, check out the Ptx3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Ptx3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Ptx3 infographic
Kit Components And Assay QC Data Details Of Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0862-CAP |
Anti-Mouse Ptx3 Pre-coated 96-well strip microplate | 1 |
| EK0862-ST | Mouse Ptx3 Standard | 2 vials, 10 ng/tube |
| EK0862-DA | Mouse Ptx3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0862 Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.035 | 0.119 | 0.209 | 0.344 | 0.626 | 1.028 | 1.542 | 2.207 |
We measured random samples of Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 262 | 1334 | 4146 | 264 | 1451 | 3917 |
| Standard deviation | 15.19 | 98.71 | 211.44 | 15.84 | 113.17 | 274.19 |
| CV (%) | 5.8% | 7.4% | 7% | 6% | 7.8% | 7% |
We measured the performance consistency of Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 262 | 259 | 249 | 272 | 260 | 8.2 | 3.1% |
| Sample 2 | 1334 | 1350 | 1444 | 1511 | 1409 | 71.99 | 5.1% |
| Sample 3 | 4146 | 4596 | 4000 | 4523 | 4316 | 250 | 5.7% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Questions and answers from customers related to EK0862 Mouse PTX3/Pentraxin 3 ELISA Kit PicoKine™
7 Related Questions
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Asked: 2020-11-09
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-11-09
Question
Q: is it okay to use heparin plasma as samples in Mouse Pentraxin 3 Picokine® ELISA Kit (Catalog # EK0862)?
Verified Customer
Asked: 2020-06-30
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Pentraxin 3 causing disruption of its protein structure. Pentraxin 3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Pentraxin 3 ELISA, treating samples with a number of anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-06-30
Question
Q: how to thaw whole blood sample for Pentraxin 3 ELISA after freezing?
Verified Customer
Asked: 2019-07-17
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Pentraxin 3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-07-17
Question
Q: if the enzyme conjugated Pentraxin 3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Pentraxin 3 antigen?
B. Gonzalez
Asked: 2019-06-11
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-06-11
Question
Q: how to proceed with the analysis of ELISA data? I have obtained Pentraxin 3 level in serum.
Verified Customer
Asked: 2018-09-14
Boster Scientific Support
Answered: 2018-09-14
Question
Q: What is the optimal O.D. value for Pentraxin 3 ELISA kit? I performed your Pentraxin 3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Pentraxin 3 even though the O.D. values are not very high?
A. Thompson
Asked: 2018-04-16
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Pentraxin 3 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Pentraxin 3 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-04-16
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Asked: 2017-06-06
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-06-06
