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Product Info Summary
| SKU: | A00314-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Superoxide Dismutase 4/CCS Antibody Picoband®
SKU/Catalog Number
A00314-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Superoxide Dismutase 4/CCS Antibody Picoband® catalog # A00314-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Superoxide Dismutase 4/CCS Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00314-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CCS, different from the related mouse sequence by six amino acids, and from the related rat sequence by seven amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00314-1 is reactive to CCS in Human, Mouse, Rat
Observed Molecular Weight
34 kDa
Calculated molecular weight
29.0 kDa
Background of CCS
Copper chaperone for superoxide dismutase is a metalloprotein that is responsible for the delivery of Cu to superoxide dismutase (SOD1). In humans the protein is encoded by the CCS gene. And this gene is mapped to chromosome 11q13 by fluorescence in situ hybridization. The CCS protein is present in mammals and most eukaryotes including yeast. The structure of CCS is composed of three distinct domains that are necessary for its function. Although CCS is important for many organisms, there are CCS independent pathways for SOD1, and many species lack CCS all together, such as C. elegans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00314-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat brain tissue, rat spleen tissue, mouse brain tissue, mouse spleen tissue, 293T whole cell
IHC: human tonsil tissue
Validation Images & Assay Conditions
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Western blot analysis of CCS using anti-CCS antibody (A00314-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: rat spleen tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse spleen tissue lysates,
Lane 5: 293T whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCS antigen affinity purified polyclonal antibody (Catalog # A00314-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCS at approximately 34KD. The expected band size for CCS is at 29KD.
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IHC analysis of CCS using anti-CCS antibody (A00314-1).
CCS was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCS Antibody (A00314-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Superoxide Dismutase 4/CCS Antibody Picoband® (A00314-1)
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5 Customer Q&As for Anti-Superoxide Dismutase 4/CCS Antibody Picoband®
Question
Does A00314-1 require dry ice during shipping?
Verified customer
Asked: 2022-05-27
Answer
The Anti-Superoxide Dismutase 4/CCS Antibody Picoband™ (A00314-1) does not require dry ice during shipping.
Boster Scientific Support
Answered: 2022-05-27
Question
Will A00314-1 anti-Superoxide Dismutase 4/CCS antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
As indicated on the product datasheet, A00314-1 anti-Superoxide Dismutase 4/CCS antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-10
Question
We are currently using anti-Superoxide Dismutase 4/CCS antibody A00314-1 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-09-13
Answer
The anti-Superoxide Dismutase 4/CCS antibody (A00314-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-09-13
Question
Is there a BSA free version of anti-Superoxide Dismutase 4/CCS antibody A00314-1 available?
Verified Customer
Verified customer
Asked: 2018-02-14
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Superoxide Dismutase 4/CCS antibody A00314-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-14
Question
Is a blocking peptide available for product anti-Superoxide Dismutase 4/CCS antibody (A00314-1)?
G. Kulkarni
Verified customer
Asked: 2015-06-30
Answer
We do provide the blocking peptide for product anti-Superoxide Dismutase 4/CCS antibody (A00314-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-06-30
