Anti-Superoxide Dismutase 4/CCS Antibody Picoband™

CCS/SOD4 antibody

Boster Bio Anti-Superoxide Dismutase 4/CCS Antibody Picoband™ catalog # A00314-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00314-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Superoxide Dismutase 4/CCS Antibody Picoband™

View all CCS/SOD4 Antibodies

SKU/Catalog Number

A00314-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Superoxide Dismutase 4/CCS Antibody Picoband™ catalog # A00314-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Superoxide Dismutase 4/CCS Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00314-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CCS, different from the related mouse sequence by six amino acids, and from the related rat sequence by seven amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A00314-1 is reactive to CCS in Human, Mouse, Rat

Applications

A00314-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

34 kDa

Calculated molecular weight

29.041kDa

Background of CCS/SOD4

Copper chaperone for superoxide dismutase is a metalloprotein that is responsible for the delivery of Cu to superoxide dismutase (SOD1). In humans the protein is encoded by the CCS gene. And this gene is mapped to chromosome 11q13 by fluorescence in situ hybridization. The CCS protein is present in mammals and most eukaryotes including yeast. The structure of CCS is composed of three distinct domains that are necessary for its function. Although CCS is important for many organisms, there are CCS independent pathways for SOD1, and many species lack CCS all together, such as C. elegans.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For CCS (Source: Uniprot.org, NCBI)

Gene Name

CCS

Full Name

Copper chaperone for superoxide dismutase

Weight

29.041kDa

Alternative Names

copper chaperone for superoxide dismutase; MGC138260; Superoxide dismutase copper chaperone CCS copper chaperone for superoxide dismutase copper chaperone for superoxide dismutase|superoxide dismutase copper chaperone

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CCS, check out the CCS Infographic

CCS infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CCS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Superoxide Dismutase 4/CCS Antibody Picoband™

Question

Does A00314-1 require dry ice during shipping?

Verified customer

Asked: 2022-05-27

Answer

The Anti-Superoxide Dismutase 4/CCS Antibody Picoband™ (A00314-1) does not require dry ice during shipping.

Boster Scientific Support

Answered: 2022-05-27

Question

Will A00314-1 anti-Superoxide Dismutase 4/CCS antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-10

Answer

As indicated on the product datasheet, A00314-1 anti-Superoxide Dismutase 4/CCS antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-10

Question

We are currently using anti-Superoxide Dismutase 4/CCS antibody A00314-1 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2018-09-13

Answer

The anti-Superoxide Dismutase 4/CCS antibody (A00314-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-09-13

Question

Is there a BSA free version of anti-Superoxide Dismutase 4/CCS antibody A00314-1 available?

Verified Customer

Verified customer

Asked: 2018-02-14

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Superoxide Dismutase 4/CCS antibody A00314-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-02-14

Question

Is a blocking peptide available for product anti-Superoxide Dismutase 4/CCS antibody (A00314-1)?

G. Kulkarni

Verified customer

Asked: 2015-06-30

Answer

We do provide the blocking peptide for product anti-Superoxide Dismutase 4/CCS antibody (A00314-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-06-30

Order DetailsPrice
A00314-1

100μg

$370
A00314-1-10ug

10μg sample (liquid)

$99
A00314-1-Biotin

100 μg Biotin conjugated

$570
A00314-1-Cy3

100 μg Cy3 conjugated

$570
A00314-1-Dylight488

100 μg Dylight488 conjugated

$570
A00314-1-Dylight550

100 μg Dylight550 conjugated

$570
A00314-1-Dylight594

100 μg Dylight594 conjugated

$570
A00314-1-FITC

100 μg FITC conjugated

$570
A00314-1-HRP

100 μg HRP conjugated

$570
A00314-1-APC

100 μg APC conjugated

$670
A00314-1-PE

100 μg PE conjugated

$670
A00314-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A00314-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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