Product Info Summary
SKU: | M00916-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Mouse |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-ATF-2 Monoclonal Antibody
SKU/Catalog Number
M00916-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ATF-2 Monoclonal Antibody catalog # M00916-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATF-2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00916-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
4F12
Isotype
IgG
Immunogen
Purified recombinant fragment of human ATF-2 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M00916-1 is reactive to ATF2 in Human, Mouse
Applications
M00916-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
54.537kDa
Background of ATF2
TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 3. ELISA validation of ATF2 using Anti-ATF-2 Monoclonal Antibody (M00916-1).
ELISA analysis of ATF-2 antibody.Click image to see more details
Figure 2. Immunohistochemistry validation of ATF2 using Anti-ATF-2 Monoclonal Antibody (M00916-1).
Immunohistochemistry (IHC) analysis of paraffin-embedded lung cancer (left) and brain tissues (right) with DAB staining using ATF-2 Monoclonal Antibody.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-ATF-2 Monoclonal Antibody M00916-1
Western Blot (WB) analysis using ATF-2 Monoclonal Antibody against NIH/3T3 cell lysate.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ATF2 (Source: Uniprot.org, NCBI)
Gene Name
ATF2
Full Name
Cyclic AMP-dependent transcription factor ATF-2
Weight
54.537kDa
Superfamily
bZIP family
Alternative Names
activating transcription factor 2formerly; ATF2; cAMP responsive element binding protein 2; cAMP-responsive element-binding protein 2; CREB2; CREBP1; CRE-BP1; cyclic AMP-dependent transcription factor ATF-2; HB16; MGC111558; mXBP; TREB7 ATF2 CRE-BP1, CREB-2, CREB2, HB16, TREB7 activating transcription factor 2 cyclic AMP-dependent transcription factor ATF-2|activating transcription factor 2 splice variant ATF2-var2|cAMP response element-binding protein CRE-BP1|cAMP responsive element binding protein 2, formerly|cAMP-dependent transcription factor ATF-2|cyclic AMP-responsive element-binding protein 2|histone acetyltransferase ATF2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATF2, check out the ATF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATF-2 Monoclonal Antibody (M00916-1)
Hello CJ!
M00916-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wu T,Liu Q,Li Y,Li H,Chen L,Yang X,Tang Q,Pu S,Kuang J,Li R,Huang Y,Zhang J,Zhang Z,Zhou J,Huang C,Zhang G,Zhao Y,Zou M,Jiang W,Mo L,He J.Feeding-induced hepatokine, Manf, ameliorates diet-induced obesity by promoting adipose browning via p38 MAPK pathway.J Exp Med.2021 Jun 7;218(6):e20201203.doi:10. 1084/jem. 20201203.PMID:33856409.
Species: Mouse
M00916-1 usage in article: APP:WB, SAMPLE:ADIPOCYTES, DILUTION :1:2000
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