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Fast ELISA kit now available: Boster is proud to announce the newest Picokine Fast ELISA kits. Now you can perform what used to be 4+ hour ELISA assay in under 1.5 hours. Available for Human P-Selectin / CD62P PicoKine™ Fast ELISA Kit and many others.
Overview
| Product Name |
Human P-Selectin / CD62P ELISA Kit PicoKine™
See all P-Selectin/CD62P primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK0505 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Human P Selectin/SELP with <5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. P Selectin/SELP tissue specificity: Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. |
| Cite This Product |
Human P-Selectin / CD62P ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0505)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates, serum and plasma(heparin, EDTA, citrate). |
| Sensitivity |
<5pg/ml |
| Assay Range |
156pg/ml-10000pg/ml |
| Immunogen/Standard |
Expression system for standard: NSO; Immunogen sequence: W42-A771 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For SELP (Source: Uniprot.org, NCBI)
| Uniprot Id | P16109 |
|---|
| NCBI Gene Id | 6403 |
|---|
| Species Of This Entry | Human |
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| Gene Name | SELP |
|---|
| Protein Name | P-selectin |
|---|
| Superfamily | selectin/LECAM family |
|---|
| Alternative Names | P-Selectin/CD62P|CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP |
|---|
| Molecular Weight | 90834 |
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| Post Tranlational Modifications | Phosphorylation; Nitration; Cleavage; Oxidation |
|---|
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. |
|---|
| Gene Location | 1q24.2, on Chromasome 1, gene sequence: NC_000001.11 (169588849..169630125, complement) |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For SELP (Source: Uniprot.org, NCBI)
| Protein Function | Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. |
|---|
| Tissue Specificity | Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. |
|---|
| Interacting Proteins | SELPLG |
|---|
| Related Diseases | Tissue Adhesions; Inflammation; Malignant Neoplasms; Atherosclerosis; Cardiovascular Diseases; Neoplasms; Heart Diseases; Inflammatory Response; Malignant Head And Neck Neoplasm; Von Willebrand Disease; Infarction; Myocardial Infarction; Head And Neck Neoplasms; Malignant Neck Neoplasm; Coronary Artery Disease; Thrombosis; Dysequilibrium Syndrome; Coronary Heart Disease; Diabetes Mellitus; Neoplasm Metastasis |
|---|
| Related Pathways | Cell Adhesion; Platelet Activation; Pathogenesis; Inflammatory Response; Platelet Aggregation; Aging; Cell Activation; Immune Response; Endothelial Cell Activation; Angiogenesis; Transport; Hypersensitivity; Virulence; Spermatogenesis; Fermentation; Translation; Cell Cycle; Fibrinolysis; Innate Immune Response; Cognition |
|---|
| Background | P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1(PSGL-1). The standard product used in this kit is recombinant human P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 730 amino acids sequence with the molecular mass of 80 KDa. |
|---|
| Scientific References | PMID: 2466574 by Johnston G.I., et al. Cloning of GMP-140, a granule membrane protein of platelets and endothelium: sequence similarity to proteins involved in cell adhesion and inflammation.
PMID: 7684381 by Fujimoto T., et al. P-selectin is acylated with palmitic acid and stearic acid at cysteine 766 through a thioester linkage. |
|---|
Kit Components And Assay QC Data Details Of Human P-Selectin / CD62P ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0505-CAP | 96-well plate precoated with anti-Human P-Selectin/CD62P antibody | 1 |
| EK0505-ST | lyophilized recombinant Human P-Selectin/CD62P standard | 10ng/tube |
| EK0505-DA | biotinylated anti-Human P-Selectin/CD62P antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0505 Human P-Selectin / CD62P ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.033 | 0.273 | 0.474 | 0.728 | 1.264 | 1.714 | 2.099 | 2.290 |
We measured random samples of Human P-Selectin / CD62P ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 322 | 1820 | 5020 | 331 | 1849 | 5389 |
| Standard deviation | 15.63 | 121.94 | 256.02 | 21.18 | 144.22 | 301.78 |
| CV(%) | 4.7% | 6.7% | 5.6% | 6.4% | 7.8% | 5.6% |
We measured the performance consistency of Human P-Selectin / CD62P ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 322 | 316 | 323 | 357 | 329 | 16.1 | 4.8% |
| Sample 2 | 1820 | 1756 | 2051 | 1775 | 1850 | 118.06 | 6.3% |
| Sample 3 | 5020 | 5825 | 5171 | 5716 | 5433 | 343.86 | 6.3% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 4
| Zhong M, He B, Yang J, Bao R, Zhang Y, Zhou D, Chen Y, Li L, Han C, Yang Y, Sun Y, Cao Y, Li Y, Shi W, Jiang S, Zhang X, Yan H. Antimicrob Agents Chemother. 2012 Jun;56(6):3121-32. Doi: 10.1128/Aac.05950-11. Epub 2012 Mar 5. L-Selectin And P-Selec... |
PubMed ID 22391529 |
| Li L, Ben Y, Yuan S, Liu A, Wu H, Xu J, Zhang X. Emerg Microbes Infect. 2013 Jul;2(7):E42. Doi: 10.1038/Emi.2013.42. Epub 2013 Jul 10. Recruitment Of Hiv-1 Target Cells At Topical Mucosal Sites: A Sensitive And Early Marker For Determining The Saf... |
PubMed ID 26038476 |
| Fayed, M.A., Abdel-Hady, H.E., Hafez, M.M., Salama, O.S., & Al-Tonbary, Y.A. (2017). Study of platelet activation, hypercoagulable state, and the association with pulmonary hypertension in children with β-thalassemia. Hematology/Oncology and Stem ... |
PubMed ID 28633041 |
| Plasma Vitamin D Status and Its Correlation with Risk Factors of Thrombosis, P-selectin and hs-CRP Level in Patients with Venous Thromboembolism; the First Study of Iranian Population |
PubMed ID 24734087 |
