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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
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Human P-Selectin / CD62P ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human P-Selectin / CD62P ELISA Kit PicoKine™ (96 Tests). Quantitate Human SELP in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human P-Selectin / CD62P ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0505)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: W42-A771
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0505 is reactive to SELP in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
EK0505 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0505-CAP||Anti-Human SELP Pre-coated 96-well strip microplate||1|
|EK0505-ST||Human SELP Standard||2 vials, 10 ng/tube|
|EK0505-DA||Human SELP Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human P-Selectin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect P-Selectin/CD62P, Dilution ratio of 1:20, concentration in serum is 51—113ng/ml.
Some articles we found to cite concentrations of P-Selectin/CD62P in samples: 25218335 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human P-Selectin / CD62P ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of P-Selectin/CD62P in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For SELP (Source: Uniprot.Org, NCBI)
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP SELP CD62, CD62P, GMP140, GRMP, LECAM3, PADGEM, PSEL selectin P P-selectin|CD62 antigen-like family member P|GMP-140|granule membrane protein 140|granule membrane protein 140kDa|granulocyte membrane protein|leukocyte-endothelial cell adhesion molecule 3|platelet activation dependent granule-external membrane protein|platelet alpha-granule membrane protein|selectin P (granule membrane protein 140kDa, antigen CD62)*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on SELP, check out the SELP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SELP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0505 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Nesibe,E.Y.,& KONUKOĞLU,D.(2021).Plasma Levels of Soluble P-Selectin, Beta-Thromboglobulin and Platelet Indices in Patients With Prediabetes: Effects of Acute Hyperglycemic Stress.Turkish Journal of Endocrinology and Metabolism,25(2),174.
EK0505 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA
Plasma Vitamin D Status and Its Correlation with Risk Factors of Thrombosis, P-selectin and hs-CRP Level in Patients with Venous Thromboembolism; the First Study of Iranian Population
Fayed, M.A., Abdel-Hady, H.E., Hafez, M.M., Salama, O.S., & Al-Tonbary, Y.A. (2017). Study of platelet activation, hypercoagulable state, and the association with pulmonary hypertension in children with β-thalassemia. Hematology/Oncology and Stem ...
Li L, Ben Y, Yuan S, Liu A, Wu H, Xu J, Zhang X. Emerg Microbes Infect. 2013 Jul;2(7):E42. Doi: 10.1038/Emi.2013.42. Epub 2013 Jul 10. Recruitment Of Hiv-1 Target Cells At Topical Mucosal Sites: A Sensitive And Early Marker For Determining The Saf...
Zhong M, He B, Yang J, Bao R, Zhang Y, Zhou D, Chen Y, Li L, Han C, Yang Y, Sun Y, Cao Y, Li Y, Shi W, Jiang S, Zhang X, Yan H. Antimicrob Agents Chemother. 2012 Jun;56(6):3121-32. Doi: 10.1128/Aac.05950-11. Epub 2012 Mar 5. L-Selectin And P-Selec...
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