Anti-Vasopressin V1a receptor AVPR1A Antibody Picoband®

Anti-AVPR1A antibody, PA2248, All Western blotting
All lanes: Anti-AVPR1A(PA2248) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Rat Kidney Tissue Lysate at 40ug
Predicted bind size: 47KD
Observed bind size: 47KD

RNA-seq reveals key molecular signatures in hippocampal tissue of DHEA-treated mice. A Cluster heatmap of differentially expressed genes between the control and DHEA-treated groups. B The total GO term analysis (Top 20) revealed that hormone activity was prioritized. C The total KEGG enrichment analysis (Top 20) showed that neuroactive ligand-receptor interaction was significantly enriched. D KEGG enrichment analysis using chord diagrams revealed several genes, such as Avpr1a, in the neuroactive ligand-receptor interaction pathway
Index in PubMed under a CC BY license. PMID: 40437391

Expression of candidate differential genes was verified by qRT-PCR in female mouse hippocampal tissues. A Candidate genes and the signaling pathways they are involved in. B-G mRNA expression levels of Aox2 , Pomc , Wnt3a , Avpr1a , Ccr10 , and P2ry10b as determined by qRT-PCR ( n = 9). The data are shown as the mean ± SEM. An unpaired Student’s t-test was used for statistical analysis, and the experiment was repeated three times independently with similar results. * indicates p < 0.05, ** indicates p < 0.01, **** indicates p < 0.0001
Index in PubMed under a CC BY license. PMID: 40437391

DHEA decreased the activity of the AVP system in the hippocampus. A Protein levels of AVPR1a in the hippocampal tissue detected by western blot (DHEA-treated vs. Control, n = 3). B Relative quantification of AVPR1a protein in the hippocampus of female mice in the DHEA-treated ( n = 3) and control ( n = 3) groups. C-D The content of AVP and OT in the hippocampus tissues of female mice in the DHEA-treated ( n = 7–8) and control ( n = 7–8) groups, as determined by ELISA. The data are shown as the mean ± SEM. An unpaired Student’s t-test was used for statistical analysis, and the experiment was repeated three times independently with similar results, * indicates p < 0.05
Index in PubMed under a CC BY license. PMID: 40437391

DHEA regulates Avpr1a transcription directly through AR. A Experimental design for ChIP-PCR. The yellow fan indicates the androgen receptor (AR). The long green line represents the Avpr1a DNA. The red rectangle indicates the binding site of the androgen to the Avpr1a promoter. B Electropherogram of a ChIP-PCR experiment. The bands in the AR group were more intense and more specific than those in the IgG group using the two primers in the PCR process. C Sequences of the primers for ChIP-PCR. The experiment was repeated three times independently with similar results
Index in PubMed under a CC BY license. PMID: 40437391

DHEA inhibited AVPR1a expression in HT22 cells via AR. A The expression of AVPR1a protein in HT22 cells treated with DHEA at different concentrations (0 µM, 10 µM and 20 µM) for different durations (24 h and 48 h) was detected by western blot. B , C The relative expression of AVPR1a protein ( B ) and mRNA ( C ) in HT22 cells treated with different concentrations (0 µM, 10 µM, and 20 µM) of DHEA for different periods (24 h and 48 h). D The expression of AVPR1a protein in DHEA-treated HT22 cells after antagonist pretreatment was detected by western blot. E , F The relative expression of AR protein ( E ) and mRNA ( F ) in DHEA-treated HT22 cells after antagonist pretreatment. G , H The relative expression of AVPR1a protein ( G ) and mRNA ( H ) in DHEA-treated HT22 cells after antagonist pretreatment. The data are shown as the mean ± SEM. Two-way ANOVA followed by Tukey’s post hoc test was used for Fig. 6B-C and one-way ANOVA followed by Tukey’s post hoc test was used for Fig. 6E-F, G-H, and the experiment was repeated three times independently with similar results. * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001, ns indicates no significant difference
Index in PubMed under a CC BY license. PMID: 40437391