Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine™

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. Cited in 273 publication(s). Independently reviewed in 3 review(s).

Product Info Summary

SKU: EK0527
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine™

See all TNF-alpha products

SKU/Catalog Number

EK0527

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0527)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L80-L235

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml (cell culture supernatants), 7.8 pg/ml - 500 pg/ml (mouse serum,plasma)

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0527 is reactive to Tnf in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0527 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0527-CAP Anti-Mouse Tnf Pre-coated 96-well strip microplate 1
EK0527-ST Mouse Tnf Standard 2 vials, 10 ng/tube
EK0527-DA Mouse Tnf Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0510.0900.1560.2810.4430.7231.2841.958

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TNF-alpha, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of TNF-alpha in samples: 25561799 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)18502991653305
Standard deviation0.823.922.120.914.3924.4
CV (%)4.6%7.8%7.4%5.7%8.3%8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TNF-alpha in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 118181719180.73.8%
Sample 250545447512.945.7%
Sample 32992942932852925.011.7%
*number of samples for each test n=16.

Gene/Protein Information For Tnf (Source: Uniprot.Org, NCBI)

Uniprot ID

P06804

Gene ID

21926

Gene Name

Tnf

Full Name

Tumor necrosis factor

Weight

25.896kDa

Superfamily

tumor necrosis factor family

Alternative Names

APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha Tnf|DI, DIF, TNF-, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tna, Tnfs, Tnfsf1a, Tnlg1f, Tnf|tumor necrosis factor|tumor necrosis factor|cachectin|tumor necrosis factor ligand 1f|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tnf, check out the Tnf Infographic

Tnf infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tnf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0527 has been cited in 273 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wu JY,Li YJ,Hu XB,Huang S,Luo S,Tang T,Xiang DX.Exosomes and biomimetic nanovesicles-mediated anti-glioblastoma therapy: A head-to-head comparison.J Control Release.2021 Jul 6;336:510-521.doi:10.1016/j.jconrel.2021.07.004.Epub ahead of print.PMID:34237399.
Species: Human,Mouse,Zebrafish
EK0527 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA

Wei,L.,Liu,Y.,Liu,Z.H.I.P.I.N.G.,Mao,Q.I.C.H.A.O.,Shi,N.,& Yang,J.I.A.N.F.E.I.(2021).Inhibitory Effects of Astragalus Polysaccharide on Myocardial Apoptosis Induced by Hypoxia or Reoxygenation in Rats.Indian Journal of Pharmaceutical Sciences,83(3),614-617.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:MYOCARDIAL CELL, DILUTION:NA

Jiang M,Xue Y,Li J,Rao K,Yan S,Li H,Chen X,Li R,Liu D.PKCδ/MAPKs and NF-κB Pathways are Involved in the Regulation of Ingenane-Type Diterpenoids from Euphorbia neriifolia on Macrophage Function.J Inflamm Res.2021;14:2681-2696https://doi.org/10.2147/JIR.S306846
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA

Yanyan Yu,Xiangqian Li,Wenpeng Hu,et al.Self-developed NF-κB inhibitor 270 protects against LPS-induced acute kidney injury and lung injury through improving inflammation.Authorea.June 22,2021.
Species: Human,Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:PLASMA AND BMDMS, DILUTION:NA

Li H,Sun H,Xu Y,Xing G,Wang X.Curcumin plays a protective role against septic acute kidney injury by regulating the TLR9 signaling pathway.Transl Androl Urol.2021 May;10(5):2103-2112.doi:10.21037/tau-21-385.PMID:34159091;PMCID:PMC8185681.
Species: Rat

Xu C,Chen H,Zhou S,Sun C,Xia X,Peng Y,Zhuang J,Fu X,Zeng H,Zhou H,Cao Y,Yu Q,Li Y,Hu L,Zhou G,Yan F,Chen G,Li J.Pharmacological Activation of RXR-α Promotes Hematoma Absorption via a PPAR-γ-dependent Pathway After Intracerebral Hemorrhage.Neurosci Bull.2021 Jun 17.doi:10.1007/s12264-021-00735-3.Epub ahead of print.PMID:34142331.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

Shicheng Xu,Anping Guo,Nan-nan Chen,Wei Dai,Huan-aoyu Yang,Wenqin Xie,Mengjie Wang,Qi-Dong You,Xiao-Li Xu,Design and synthesis of Grp94 selective inhibitors based on Phe199 induced fit mechanism and their anti-inflammatory effects,European Journal of Medicinal Chemistry,2021,113604,ISSN 0223-5234,https://doi.org/10.1016/j. ejmech.2021.113604.
Species: Mouse,Rat
EK0527 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION: NA

Zhang,J.,Zhu,L.,Shi,H.,& Zheng,H.(2021).Protective effects of miR‑155‑5p silencing on IFN‑γ‑induced apoptosis and inflammation in salivary gland epithelial cells.Experimental and Therapeutic Medicine,22,882.https://doi.org/10.3892/etm.2021.10314
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE: SGECS, DILUTION:NA

Tawfiq,R.A.,Attia,Y.M.,Nassar,N.N.,Hammam,O.A.,Elmazar,M.M.,& Abdallah,D.M.(2021).Insights into the Impact of FXR Activation on Hepatic Autophagy in a Non-alcoholic Steatohepatitis Model.Bulletin of Pharmaceutical Sciences.Assiut,44(1),265-274.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:LIVER TISSUE, DILUTION:NA

Hsu WH,Lin LJ,Lu CK,Kao ST,Lin YL.Effect of You-Gui-Wan on House Dust Mite-Induced Mouse Allergic Asthma via Regulating Amino Acid Metabolic Disorder and Gut Dysbiosis.Biomolecules.2021 May 30;11(6):812.doi:10.3390/biom11060812.PMID:34070764.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Xingwei Xiang,Xiaoling Zhou,Wenjie Wang,Yufang Zhou,Xuxia Zhou,Shanggui Deng,Bin Zheng,Zhengshun Wen,Effect of Antarctic Krill Phospholipid (KOPL) on High Fat Diet-Induced Obesity in Mice,Food Research International,2021,110456,ISSN 0963-9969,https://doi.org/10.1016/j.foodres.2021.110456.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

WEI Wen-Long,WU Shi-Fei,LI Hao-Jv,LI Zhen-Wei,QU Hua,YAO Chang-Liang,ZHANG Jian-Qing,LI Jia-Yuan,WU Wan-Ying , GUO De-An.Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS [J].Chin J Nat Med,2021,19(6):473-480.doi:10.1016/S1875-5364(21)60046-8
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA

Zhang W,Li J,Yao H,Li T.Restoring microRNA-499-5p Protects Sepsis-Induced Lung Injury Mice Via Targeting Sox6.Nanoscale Res Lett.2021 May 21;16(1):89.doi:10.1186/s11671-021-03534-x.PMID:34019224.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA

Zheng,W.,Xie,Q.,Zhang,Z.et al.Inhibited HDAC3 or Elevated MicroRNA-494-3p Plays a Protective Role in Myocardial Ischemia-Reperfusion Injury via Suppression of BRD4.Mol Neurobiol (2021).https://doi.org/10.1007/s12035-021-02369-y
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Li D,Weng Y,Wang G,Zhen G.Anti-Septic Potential of 7-α-Obacunyl Acetate Isolated from the Toona sinensis on Cecal Ligation/Puncture Mice via Suppression of JAK-STAT/NF-κB Signal Pathway.Infect Drug Resist.2021;14:1813-1821https://doi.org/10.2147/IDR.S302853
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Wang Y,Gao Y,Huang Y,Pan Y,Yu Y,Zhou Y,Wan SS,Yang YN.The potential protective effects of miR-497 on corneal neovascularization are mediated via macrophage through the IL-6/STAT3/VEGF signaling pathway.Int Immunopharmacol.2021 May 10;96:107745.doi:10.1016/j.intimp.2021. 107745. Epub ahead of print.PMID:33984719.
Species: Human,Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:CORNEAL TISSUE, DILUTION:NA

Ai Z,Cheng C,Zhou L,Yin S,Wang L,Liu Y.Bone marrow mesenchymal stem cells-derived extracellular vesicles carrying microRNA-221-3p protect against ischemic stroke via ATF3.Brain Res Bull.2021 Apr 28:S0361-9230(21)00125-8.doi:10.1016/j.brainresbull.2021.04.022.Epub ahead of print.PMID:33932490.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:BMSCS AND BRAIN TISSUE, DILUTION:NA

Yilun Luo,Xiaowen Zheng,Peiqi Yuan,Xingyao Ye,Lie Ma,Light-induced dynamic RGD pattern for sequential modulation of macrophage phenotypes,Bioactive Materials,Volume 6,Issue 11,2021,Pages 4065-4072,ISSN 2452-199X,https://doi.org/10.1016/j.bioactmat.2021.04.018.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:BMDM, DILUTION:NA

Duan Y,Zheng H,Li Z,Yao Y,Ding J,Wang X,Nakkala JR,Zhang D,Wang Z,Zuo X,Zheng X,Ling J,Gao C.Unsaturated polyurethane films grafted with enantiomeric polylysine promotes macrophage polarization to a M2 phenotype through PI3K/Akt1/mTOR axis.Biomaterials.2020 Jul;246:120012.doi:10.1016/j.biomaterials.2020.120012.Epub 2020 Mar 31.PMID:32276198.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:MACROPHAGES, DILUTION: NA

Guo J,Li M,Yang Y,Zhang L,Zhang LW,Sun QY.Pretreatment with atorvastatin ameliorates cobra venom factor-induced acute lung inflammation in mice.BMC Pulm Med.2020 Oct 12;20(1):263.doi:10.1186/s12890-020-01307-3.PMID:33046059;PMCID:PMC7552367.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA

Feng Sun,Haiwei Zhang,Tianwen Huang,Jianhui Shi,Tianli Wei,Yansong Wang,"miRNA-221 Regulates Spinal Cord Injury-Induced Inflammatory Response through Targeting TNF-α Expression",BioMed Research International,vol.2021,Article ID 6687963,6 pages,2021.https://doi.org/10.1155/2021/6687963
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:SPINAL CORD TISSUE, DILUTION:NA

Zhu Y,Liang H,Liu X,Wu J,Yang C,Wong TM, Kwan KYH,Cheung KMC,Wu S,Yeung KWK.Regulation of macrophage polarization through surface topography design to facilitate implant-to-bone osteointegration. Sci Adv.2021 Apr 2;7(14):eabf6654.doi:10. 1126/sciadv.abf6654.PMID:33811079.
Species: Mouse,Rat
EK0527 usage in article: APP: ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA

Li Y, Wang N, Ma Z, Wang Y, Yuan Y, Zhong Z, Hong Y, Zhao M. Lipoxin A4 protects against paraquat‑induced acute lung injury by inhibiting the TLR4/MyD88‑mediated activation of the NF‑κB and PI3K/AKT pathways. Int J Mol Med. 2021 May;47(5):86. doi: 10.3892/ijmm. 2021.4919. Epub 2021 Mar 24. PMID: 33760150.
Species: Mouse,Rat
EK0527 usage in article: APP:ELISA, SAMPLE:BALF AND RAW264.7 CELL, DILUTION: NA

Li-Bo Jiang, Sheng-Long Ding, Wang Ding, Di-Han Su, Fang-Xue Zhang, Tai-Wei Zhang, Xiao-Fan Yin, Lan Xiao, Yu-Lin Li, Feng-Lai Yuan, Jian Dong, Injectable Sericin Based Nanocomposite Hydrogel for Multi-modal Imaging-guided Immunomodulatory Bone Regeneration,Chemical Engineering Journal,2021,129323,ISSN 1385-8947,https://doi.org/10.1016/j.cej.2021.129323.
Species: Mouse,Rat
EK0527 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA

He Y, Peng H, Zhang H, Liu Y, Sun H. Structural characteristics and immunopotentiation activity of two polysaccharides from the petal of Crocus sativus. Int J Biol Macromol. 2021 Mar 4:S0141-8130(21)00523-7. doi: 10.1016/j.ijbiomac.2021.03. 006. Epub ahead of print. PMID: 33676979.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA

Pingjun Zhu,Kun Wan,Ming Yin,Peng Hu,Yifan Que,Xin Zhou,Lei Zhang,Tianzhi Li,Yingzhen Du,Guogang Xu,Xiangqun Fang,"RIPK3 Induces Cardiomyocyte Necroptosis via Inhibition of AMPK-Parkin-Mitophagy in Cardiac Remodelling after Myocardial Infarction",Oxidative Medicine and Cellular Longevity,vol.2021,Article ID 6635955,11 pages,2021.https://doi.org/10.1155/2021/6635955
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Yin S, Li L, Tao Y, Yu J, Wei S, Liu M and Li J (2021) The Inhibitory Effect of Artesunate on Excessive Endoplasmic Reticulum Stress Alleviates Experimental Colitis in Mice. Front. Pharmacol. 12:629798. doi: 10.3389/fphar.2021.629798
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA

Jie Cheng, Min Chen, Hui-Qi Wan, Xue-Qin Chen, Cheng-Fu Li, Ji-Xiao Zhu, Qing Liu, Guang-Hui Xu, Li-Tao Yi,Paeoniflorin exerts antidepressant-like effects through enhancing neuronal FGF-2 by microglial inactivation,Journal of Ethnopharmacology,2021,114046,ISSN 0378-8741,https://doi.org/10.1016/j.jep.2021.114046.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPUS TISSUE, DILUTION:NA

Yang, Y., Deng, R., Chen, Z., Yao, L., Yang, X., & Xiang, D. (2021). Piperazine ferulate attenuates high glucose‑induced mesangial cell injury via the regulation of p66Shc. Molecular Medicine Reports, 23, 374. https://doi.org/10.3892/mmr.2021.12013
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:SV-40 CELL AND MES-13 CELL, DILUTION:NA

Liu Hui,Zhou Chuankun,Qi Dahu,Gao Yutong,Zhu Meipeng,Tao Tenghui,Sun Xuying,Xiao Jun,Inhibiting Monoacylglycerol Lipase Suppresses RANKL-Induced Osteoclastogenesis and Alleviates Ovariectomy-Induced Bone Loss.Frontiers in Cell and Developmental Biology,VOLUME=9,YEAR=2021, PAGES=544, URL=https://www.frontiersin.org/article/10.3389/fcell.2021.640867. DOI=10.3389/fcell.2021.640867,ISSN=2296-634X
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Fu DN,Wang Y,Yu LJ, Liu MJ,Zhen D.Silenced long non-coding RNA activated by DNA damage elevates microRNA-495-3p to suppress atherosclerotic plaque formation via reducing Krüppel-like factor 5.Exp Cell Res.2021 Feb 23: 112519.doi:10.1016/j.yexcr.2021.11251
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Zhang G,Li T,Chang X,Xing J.Long Noncoding RNA SNHG14 Promotes Ischemic Brain Injury via Regulating miR-199b/AQP4 Axis.Neurochem Res.2021 Feb 20.doi:10.1007/s11064-021-03265-6.Epub ahead of print.PMID:33609254.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:BV2 CELL, DILUTION:NA

Liu Y,Qi Y.Vildagliptin, a CD26/DPP4 inhibitor, ameliorates bleomycin-induced pulmonary fibrosis via regulating the extracellular matrix.Int Immunopharmacol.2020 Oct;87:106774.doi:10.1016/j.intimp.2020.106774.Epub 2020 Jul 27. PMID:32731178.
Species: Human,Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:PLASMA AND LUNG TISSUE, DILUTION:NA

Liu H,Hu X,Jiang R,Cai J,Lin Q,Fan Z,Zhao P,Wang S,Zou C,Du W,Dong Z,Liu Y.CQMUH-011 Inhibits LPS-Induced Microglia Activation and Ameliorates Brain Ischemic Injury in Mice.Inflammation.2021 Feb 2.doi:10.1007/s10753-021-01420-3.Epub ahead of print.PMID:33
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:MICROGLIAL CELL AND BRAIN TISSUE, DILUTION:NA

Zou XY,Xie RR,Li W,Su CL,Chen YS,Tang H.Novel sampangine derivatives as potent inhibitors of Cu2+-mediated amyloid-β protein aggregation, oxidative stress and inflammation.Int J Biol Macromol.2021 Jan 18:S0141-8130(21)00123-9.doi:10.1016/j.ijbiomac.2021.0
Species: Mouse,Worms
EK0527 usage in article: APP:ELISA, SAMPLE:BV2 CELL, DILUTION:NA

Sharma D,Arora S,Banerjee A,Singh J.Improved insulin sensitivity in obese-diabetic mice via chitosan Nanomicelles mediated silencing of pro-inflammatory Adipocytokines.Nanomedicine.2021 Jan 15:102357.doi:10.1016/j.nano.2020.102357.Epub ahead of print. PMI
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:ADIPOSE TISSUE, DILUTION:NA

Cao Y,Li Y,He C,Yan F,Li JR,Xu HZ,Zhuang JF,Zhou H,Peng YC,Fu XJ,Lu XY,Yao Y,Wei YY,Tong Y,Zhou YF,Wang L.Selective Ferroptosis Inhibitor Liproxstatin-1 Attenuates Neurological Deficits and Neuroinflammation After Subarachnoid Hemorrhage.Neurosci Bull.202
Species: Human,Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

Zheng Q,Zhang J,Zuo X,Sun J,Liang Z,Hu X,Wang Z,Li K,Song J,Ding T,Shen X,Ma Y,Li P.Photobiomodulation Promotes Neuronal Axon Regeneration After Oxidative Stress and Induces a Change in Polarization from M1 to M2 in Macrophages via Stimulation of CCL2 in
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE: MACROPHAGES, DILUTION:NA

Li Y,Song W,Tong Y,Zhang X,Zhao J,Gao X,Yong J,Wang H.Isoliquiritin ameliorates depression by suppressing NLRP3-mediated pyroptosis via miRNA-27a/SYK/NF-κB axis.J Neuroinflammation.2021 Jan 5;18(1):1.doi:10.1186/s12974-020-02040-8.PMID:33402173;PMCID:PMC7
Species: Human,Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:SERUM AND HIPPOCAMPUS TISSUE, DILUTION:NA

Chen X,He Y,Zhu Y,Du J,Sun H.linc-AAM Facilitates Gene Expression Contributing to Macrophage Activation and Adaptive Immune Responses.Cell Rep.2021 Jan 5;34(1):108584.doi:10.1016/j.celrep.2020.108584.PMID:33406422.
Species: Human,Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA

Wang W,Xu RL,He P,Chen R.MAR1 suppresses inflammatory response in LPS-induced RAW 264.7 macrophages and human primary peripheral blood mononuclear cells via the SIRT1/PGC-1α/PPAR-γ pathway.J Inflamm (Lond).2021 Feb 8;18(1):8.doi:10.1186/s12950-021-00271-x
Species: Human,Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA

Wu,H.,Jiang,X.,Li,Y.,Zhou,Y.,Zhang,T.,Zhi,P.,Gao,J.,Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery.Adv. Funct.Mater.2020, 30, 2006169.https://doi.org/10.1002/adfm.202006169
Species: Rat,Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

Dong-Wei Wang,Shu-Juan Li,Xue-Ying Tan,Jin-Hui Wang,Ying Hu,Zeng Tan,Jing Liang,Jing-Bo Hu,Yan-Guo Li,Yu-Fen Zhao,Engineering of stepwise-targeting chitosan oligosaccharide conjugate for the treatment of acute kidney injury,Carbohydrate Polymers,Volume 25
Species: Human,Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:RENAL TISSUE, DILUTION:NA

Xin Li,Licong Yang,Meng Xu,Gaoxiang Qiao,ChangJiang Li,Lezhen Lin,Guodong Zheng,Smilax china L. polyphenols alleviates obesity and inflammation by modulating gut microbiota in high fat/high sucrose diet-fed C57BL/6J mice,Journal of Functional Foods,Volume
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Long-Wei Li,Xiao-Yi Chen,Lan-Cong Liu,Yi Yang,You-Jiao Wu,Gang Chen,Zhi-Feng Zhang,Pei Luo,Oil-in-water camellia seeds oil nanoemulsions via high pressure microfluidization: Formation and evaluation,LWT,2020, 110815,ISSN 0023-6438,https://doi.org/10.1016/
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Tao Huang,Yurong Zhang,Wenjuan Zhang,Tingting Lin,Luoting Chen,Bei Yang,Lei Wu,Jianhua Yang,Dalei Zhang,"Attenuation of Perfluorooctane Sulfonate-Induced Steatohepatitis by Grape Seed Proanthocyanidin Extract in Mice",BioMed Research International,vol.202
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:HEPATIC TISSUE, DILUTION:NA

Mingyue Shen,Xianxiang Chen,Lixin Huang,Qiang Yu,Yi Chen,Jianhua Xie,Sulfated Mesona chinensis Benth polysaccharide enhance the immunomodulatory activities of cyclophosphamide-treated mice,Journal of Functional Foods,Volume 76,2021,104321,ISSN 1756-4646,h
Species: Mouse
EK0527 usage in article: APP:RAW264.7 CELLS, DILUTION:NA

Mu,D,Li,D,Li,J,et al. Long non‐coding RNA HULC protects against atherosclerosis via inhibition of PI3K/AKT signaling pathway. IUBMB Life.2020;1– 11.https://doi.org/10.1002/iub.2417
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:AORTIC VASCULAR TISSUE, DILUTION:NA

Dai,J,Chen,J,Qi,J, et al. Konjac Glucomannan from Amorphophallus konjac enhances immunocompetence of the cyclophosphamide‐induced immunosuppressed mice. Food Sci Nutr. 2020; 00: 1– 8. https://doi.org/ 10.1002/fsn3.2038
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Xinxin Zhang,Yating Qin,Xiaoning Wan et al. Rosuvastatin Inhibits Atherosclerosis Development by Improving Lipid Accumulation and Polarization Conversion of Macrophages via Regulating Autophagy, 19 November 2020,PREPRINT (Version 1) available at Research
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Tailin He, Jialin Shang, Chenglong Gao, Xin Guan, Yingyi Chen, Liwen Zhu, Luyong Zhang, Cunjin Zhang, Jian Zhang, Tao Pang, A novel SIRT6 activator ameliorates neuroinflammation and ischemic brain injury via EZH2/FOXC1 axis, Acta Pharmaceutica Sinica B,20
Species: Human,Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELLS, MURINE MACROPHAGE CELLS AND HUMAN PBMC, DILUTION:NA

Wang X,Tong Y,Zhang J,Khan N,Zhang K,Bai H,Zhang Q,Chen Y.Neuroinflammation changes with periodontal inflammation status during periodontitis in wild-type mice.Oral Dis.2020 Aug 20.doi:10.1111/odi.13618.Epub ahead of print.PMID:32815656.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPI, DILUTION:NA

Yang L,Liu G,Fu L,Zhong W,Li X,Pan Q. DNA repair enzyme OGG1 promotes alveolar progenitor cell renewal and relieves PM2.5-induced lung injury and fibrosis. Ecotoxicol Environ Saf.2020 Dec 1;205:111283.doi: 10.1016/j.ecoenv.2020.111283. Epub 2020 Sep 22.PM
Species: Human,Mouse

Kong Y,Peng Q,Lv N,Yuan J,Deng Z,Liang X,Chen S,Wang L.Paeoniflorin exerts neuroprotective effects in a transgenic mouse model of Alzheimer‘s disease via activation of adenosine A1 receptor. Neurosci Lett.2020 Jun 21;730:135016.doi:10.1016/j.neulet.
Species: Mouse

Gao Y,Zhou X,Zhou Y,Zhang W,Zhao L.Chrysene accelerates the proceeding of chronic obstructive pulmonary disease with the aggravation of inflammation and apoptosis in cigarette smoke exposed mice.Hum Exp Toxicol.2020 Dec 20:960327120979343.doi:10. 1177/096
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:LUNG TISSUE, DILUTION:NA

Liao H,Li Y,Zhang X,Zhao X,Zheng D,Shen D,Li R. Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation.J Immunol Res.2020 Aug 27;2020:8263598.doi:10.1155/2020/ 8263598.PMID
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE, PODOCYTES, DILUTION:NA

Li Y,Zheng D,Shen D,Zhang X,Zhao X,Liao H.Protective Effects of Two Safflower Derived Compounds, Kaempferol and Hydroxysafflor Yellow A, on Hyperglycaemic Stress-Induced Podocyte Apoptosis via Modulating of Macrophage M1/M2 Polarization.J Immunol Res.2020
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:PODOCYTES, DILUTION:NA

Zhu J, Yang LK, Chen WL, Lin W, Wang YH, Chen T. Activation of SK/KCa Channel Attenuates Spinal Cord Ischemia-Reperfusion Injury via Anti-oxidative Activity and Inhibition of Mitochondrial Dysfunction in Rabbits. Front Pharmacol. 2019 Apr 2;10:325. doi: 1
Species: Rabbit
EK0527 usage in article: APP:ELISA, SAMPLE:SPINAL CORD, DILUTION:NA

He L,Wang X,Cheng D,Xiong Z,Liu X. Ginsenoside Rg1 improves pathological damages by activating the p21‑p53‑STK pathway in ovary and Bax‑Bcl2 in the uterus in premature ovarian insufficiency mouse models. Mol Med Rep.2021 Jan;23(1):37.doi:10.3892/mmr.2020.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:OVARY AND UTERUS TISSUE, DILUTION:NA

Zhao S, Fan Z, Hu J, Zhu Y, Lin C, Shen T, Li Z, Li K, Liu Z, Chen Y, Zhang B. The differential effects of isoflurane and sevoflurane on neonatal mice. Sci Rep. 2020 Nov 9;10(1):19345. doi: 10.1038/s41598-020-76147-6. PMID: 33168900; PMCID: PMC7652873.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

Dai Z,Chen XY,An LY,Li CC,Zhao N,Yang F,You ST,Hou CZ,Li K,Jiang C,You QD,Di B,Xu LL. Development of Novel Tetrahydroquinoline Inhibitors of NLRP3 Inflammasome for Potential Treatment of DSS-Induced Mouse Colitis. J Med Chem. 2020 Dec 17.doi: 10.1021/acs.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:THP-M CELL AND SERUM, DILUTION:NA

Zhang Z,Jiang S,Tian H,Zeng Y,He K,Lin L,Yu F. Ethyl acetate fraction from Nymphaea hybrida Peck modulates inflammatory responses in LPS-stimulated RAW 264.7 cells and acute inflammation murine models. J Ethnopharmacol. 2020 Dec 15:113698.doi:10.1016/j.je
Species: Mouse, Nymphaea hybrida
EK0527 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELLS, DILUTION:NA

Zheng B, Ying M, Xie J, Chen Y, Wang Y, Ding X, Hong J, Liao W, Yu Q. A Ganoderma atrum polysaccharide alleviated DSS-induced ulcerative colitis by protecting the apoptosis/autophagy-regulated physical barrier and the DC-related immune barrier. Food Funct
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA

Zhou Q, Guo D, Li X, Wang Y, Ye X, Xue S, Wang X. Anti-inflammatory effects of vinpocetine in LPS-stimulated microglia via activation of AMPK. An Acad Bras Cienc. 2020 Nov 20;92(4):e20200241. doi: 10.1590/0001-3765202020200241. PMID: 33237143.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:BV2 MICROGLIA, DILUTION:NA

Sun HJ, Xiong SP, Cao X, Cao L, Zhu MY, Wu ZY, Bian JS. Polysulfide-mediated sulfhydration of SIRT1 prevents diabetic nephropathy by suppressing phosphorylation and acetylation of p65 NF-κB and STAT3. Redox Biol. 2020 Nov 25;38:101813. doi: 10.1016/j.redo
Species: Mouse, Human
EK0527 usage in article: APP:ELISA, SAMPLE:RENAL TISSUE, DILUTION:NA

Du Y, Liu G, Zhao L, Yao R. Protective Effect of miR-204 on Doxorubicin-Induced Cardiomyocyte Injury via HMGB1. Oxid Med Cell Longev. 2020 Nov 19;2020:8819771. doi: 10.1155/2020/8819771. PMID: 33274007; PMCID: PMC7695502.
Species: Mouse, Rat
EK0527 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Huang Y, Tang J, Cai Z, Zhou K, Chang L, Bai Y, Ma Y. Prevotella Induces the Production of Th17 Cells in the Colon of Mice. J Immunol Res. 2020 Nov 1;2020:9607328. doi: 10.1155/2020/9607328. PMID: 33204736; PMCID: PMC7657696.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:BMDC CELLS, DILUTION:NA

Cui L, Guan X, Ding W, Luo Y, Wang W, Bu W, Song J, Tan X, Sun E, Ning Q, Liu G, Jia X, Feng L. Scutellaria baicalensis Georgi polysaccharide ameliorates DSS-induced ulcerative colitis by improving intestinal barrier function and modulating gut microbiota
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:COLON EXTRACTS, DILUTION:NA

Wu X, Lin L, Wu H. Ferulic acid alleviates lipopolysaccharide-induced acute lung injury through inhibiting TLR4/NF-κB signaling pathway. J Biochem Mol Toxicol. 2020 Nov 2:e22664. doi: 10.1002/jbt.22664. Epub ahead of print. PMID: 33140555.
Species: Mouse
EK0527 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA

Cao, Y., Cao, W., Qiu, Y., Zhou, Y., Guo, Q., Gao, Y., & Lu, N. (2020). Oroxylin A suppresses ACTN1 expression to inactivate cancer-associated fibroblasts and restrain breast cancer metastasis. Pharmacological Research, 104981.

Lu, Mengchen, et al. "A hydrogen peroxide responsive prodrug of Keap1-Nrf2 inhibitor for improving oral absorption and selective activation in inflammatory conditions." Redox Biology (2020): 101565.

Huang L,Shen M,Wu T,Yu Y,Yu Q,Chen Y,Xie J.Mesona chinensis Benth polysaccharides protect against oxidative stress and immunosuppression in cyclophosphamide-treated mice via MAPKs signal transduction pathways.Int J Biol Macromol.2020 Jun 1;152:766-774. do
Species: Mesona chinensis, Mouse

Relationship between expression of COX-2, TNF-α, IL-6 and autoimmune-type recurrent miscarriage.

IL-6 induced lncRNA MALAT1 enhances TNF-α expression in LPS-induced septic cardiomyocytes via activation of SAA3.

Litopenaeus vannamei hemocyanin exhibits antitumor activity in S180 mouse model in vivo

Melanoma B16-F1 cells coated with fusion protein of mouse calreticulin and virus G-protein coupled receptor induced the antitumor immune response in Balb/C mice

Qishen granules inhibit myocardial inflammation injury through regulating arachidonic acid metabolism

Sublytic C5b-9 Induces Glomerular Mesangial Cell Apoptosis through the Cascade Pathway of MEKK2%u2013p38 MAPK%u2013IRF-1%u2013TRADD%u2013Caspase 8 in Rat Thy-1 %u2026

Cytotoxic, antitumor and immunomodulatory effects of the water-soluble polysaccharides from lotus (Nelumbo nucifera Gaertn.) seeds

Treatment of experimental non%u2011alcoholic steatohepatitis by targeting %u03B17 nicotinic acetylcholine receptor%u2011mediated inflammatory responses in mice

Whey Acidic Protein/Four-Disulfide Core Domain 21 Regulate Sepsis Pathogenesis in a Mouse Model and a Macrophage Cell Line via the Stat3/Toll-Like Receptor 4 %u2026

Ursolic acid protects against ulcerative colitis via anti-inflammatory and antioxidant effects in mice

Exclusive enteral nutrition protects against inflammatory bowel disease by inhibiting NF%u2011%u03BAB activation through regulation of the p38/MSK1 pathway

Nobiletin attenuates lipopolysaccharide/D%u2011galactosamine%u2011induced liver injury in mice by activating the Nrf2 antioxidant pathway and subsequently inhibiting NF%u2011%u03BAB %u2026

Ascorbic acid ameliorates oxidative stress and inflammation in dextran sulfate sodium-induced ulcerative colitis in mice.

Cold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathway?in vitro

Detoxication mechanisms of Radix Tripterygium wilfordii via compatibility with Herba Lysimachia christinae in S180-bearing mice by involving Nrf2

Grape seed proanthocyanidin extract supplementation affects exhaustive exercise-induced fatigue in mice

3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress via ERK1/2 Phosphorylation

Toxoplasma gondii excretory/secretory antigens (TgESAs) suppress pro-inflammatory cytokine secretion by inhibiting TLR-induced NF-?B activation in LPS-stimulated murine macrophages

Tauroursodeoxycholic acid attenuates endoplasmic reticulum stress and protects the liver from chronic intermittent hypoxia induced injury

Effects of Recombinant Toxoplasma gondii Citrate Synthase I on the Cellular Functions of Murine Macrophages In vitro

Evaluation of the chemical composition, antioxidant and anti-inflammatory activities of distillate and residue fractions of sweet basil essential oil

Involvement of receptor-interacting protein 140 in palmitate-stimulated macrophage infiltration of pancreatic beta cells

Role of serotonin on the intestinal mucosal immune response to stress-induced diarrhea in weaning mice

Induced migration of endothelial cells into 3D scaffolds by chemoattractants secreted by pro-inflammatory macrophages in situ

Resveratrol protects against L-arginine-induced acute necrotizing pancreatitis in mice by enhancing SIRT1-mediated deacetylation of p53 and heat shock factor 1

Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells

Liver X Receptor Agonist TO901317 Attenuates Paraquat-Induced Acute Lung Injury through Inhibition of NF-?B and JNK/p38 MAPK Signal Pathways

High-Throughput Sequencing and Co-Expression Network Analysis of lncRNAs and mRNAs in Early Brain Injury Following Experimental Subarachnoid Haemorrhage

Enhanced efficacy with reduced toxicity of chemotherapy drug 5-fluorouracil by synergistic treatment with Abnormal Savda Munziq from Uyghur medicine

A novel pH?controlled hydrogen sulfide donor protects gastric mucosa from aspirin?induced injury

H9N2 Avian Influenza Virus Protein PB1 Enhances the Immune Responses of Bone Marrow-Derived Dendritic Cells by Down-Regulating miR375

Impact of bone marrow-derived mesenchymal stem cells on remodeling the lung injury induced by lipopolysaccharides in mice

5-ASA-loaded SiO2 nanoparticles-a novel drug delivery system targeting therapy on ulcerative colitis in mice

Daphnetin Protects against Cerebral Ischemia/Reperfusion Injury in Mice via Inhibition of TLR4/NF-?B Signaling Pathway

NEMO-Binding Domain Peptide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting the NF-?B Signaling Pathway

In Vitro Anti-Inflammatory Effects of Three Fatty Acids from Royal Jelly

MicroRNA-155 in exosomes secreted from helicobacter pylori infection macrophages immunomodulates inflammatory response

miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBP?

Anti-inflammatory actions of (-)-epicatechin in the adipose tissue of obese mice

LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling

Keratin 8 limits TLR-triggered inflammatory responses through inhibiting TRAF6 polyubiquitination

Surfactin induces maturation of dendritic cells in vitro

Potential implications of Apolipoprotein E in early brain injury after experimental subarachnoid hemorrhage: Involvement in the modulation of blood-brain barrier integrity

Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration

Protective Effect of Irisin on Atherosclerosis via Suppressing Oxidized Low Density Lipoprotein Induced Vascular Inflammation and Endothelial Dysfunction

MiR674 inhibits the neuraminidase-stimulated immune response on dendritic cells via down-regulated Mbnl3

4,4'-diaponeurosporene, a C30 carotenoid, effectively activates dendritic cells via CD36 and NF-?B signaling in a ROS independent manner

Synergistic Effect of Compounds from a Chinese Herb: Compatibility and Dose Optimization of Compounds from N-Butanol Extract of Ipomoea stolonifera

Protective Effect of an Antibody against Specific Extracellular Domain of TLR2 on Agonists-Driven Inflammatory and Allergic Response

In vitro modeling of COPD inflammation and limitation of p38 inhibitor - SB203580

Evaluation of immunopathologic effects of aqueous extract of?Echinacea purpurea?in mice after experimental challenge with?Pasteurella multocida?serotype A

PER1 prevents excessive innate immune response during endotoxin-induced liver injury through regulation of macrophage recruitment in mice

Inhibition of Acute Lung Injury by TNFR-Fc through Regulation of an Inflammation-Oxidative Stress Pathway

Reduction Impairs the Antibacterial Activity but Benefits the LPS Neutralization Ability of Human Enteric Defensin 5

Establishment of a novel rat model of severe acute cholangitis

Chemopreventive activity of GEN-27, a genistein derivative, in colitis-associated cancer is mediated by p65-CDX2-?-catenin axis

Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition

A deleterious role for Th9/IL-9 in hepatic fibrogenesis

Construction and identification of an RNA interference lentiviral vector targeting the mouse TNF-? gene

Immunostimulative Activity of Low Molecular Weight Chitosans in RAW264.7 Macrophages

Intervening TNF-? via PPAR? with Gegenqinlian Decoction in Experimental Nonalcoholic Fatty Liver Disease

Unfractionated heparin attenuates intestinal injury in mouse model of sepsis by inhibiting heparanase

3-(3-Pyridylmethylidene)-2-indolinone Reduces the Severity of Colonic Injury in a Murine Model of Experimental Colitis

Dietary effects on liver tumor burden in mice treated with the hepatocellular carcinogen diethylnitrosamine

Toll-like receptor 4 implicated in acute lung injury induced by paraquat poisoning in mice

Antenatal exposure of maternal secondhand smoke (SHS) increases fetal lung expression of RAGE and induces RAGE-mediated pulmonary inflammation

RNF26 Temporally Regulates Virus-Triggered Type I Interferon Induction by Two Distinct Mechanisms

Synthesis and hepatoprotective properties of?Acanthus ilicifolius?alkaloid A and its derivatives

The anti-inflammatory effects of heat shock protein 72 involve inhibition of high-mobility-group box 1 release and proinflammatory function in macrophages.

Effect of Gui Zhi decoction on enteric mucosal immune in mice with collagen-induced arthritis

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3 Reviews For Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine™

0

"Great TNF-Alpha Kit from Boster!"

Excellent

Source: Biocompare.com

"Great TNF-Alpha Kit from Boster!"

--Harrison Esterly, Appalachian State University, Chemistry, Lab Technician

Application ELISA
Sample Mouse serum
Detection --

"The kit contained an extra standard which is very useful if you need to optimize your dilution factor before you use the rest of your samples. The instructions were very easy to follow and the results we obtained were consistent between replicates. Very easy to use. Don't waste your time or money [on other kits]; just use this kit." (Customer’s Tip: "Optimization dilution factors should range from 1:5 to 1:200.")

Verified customer

Submitted

0

"Excellent Quantification In Vitro and In Vivo"

Excellent

Source: Biocompare.com

"Excellent Quantification In Vitro and In Vivo"

--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student

Application ELISA
Sample Cell culture media, mouse serum
Detection --

"I was able to estimate TNFa in cell culture media before and after treatment with lipopolysaccharides in primary macrophage cell line. In vivo estimation was done in control and treated group of mice (high fat diet treatment) by collecting blood, separating serum, and estimating the cytokine levels in serum. Overall reliable estimation was achieved. Reliable reproducible result and brand of ELISA kits. Good ELISA kit. Reliable vendor. Good continuity between different batches of ELISA kits. Will continue to buy from Boster Biological." (Customer’s Tip: "Do a test run of sample and quantities may be higher than the standard plot range.")

Verified customer

Submitted

0

"Great TNF alpha Kit"

Excellent

Source: Biocompare.com

"Great TNF alpha Kit"

--Tho Pham, UCONN, NUTRITIONAL SCIENCES, GA

Applications ELISA
Sample Cell culture supernatant
Detection --

"This ELISA kit worked as advertised. Wells can be removed as strips, so not everything has to be used. Great product. Results are good and have low %CV. Quick assay kit and cheaper than other companies in my opinon."

Verified customer

Submitted

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14 Customer Q&As for Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine™

Question

What host species of antibody used in EK0527- TNFa (mouse) ELISA Kit?

Verified customer

Asked: 2020-06-18

Answer

For the Mouse TNF Alpha ELISA Kit PicoKine™ (EK0527), Host of capture antibody: Hamster Host of detection antibody: Goat

Boster Scientific Support

Answered: 2020-06-18

Question

Q: if the enzyme conjugated TNF-alpha antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TNF-alpha antigen?

Y. Banerjee

Verified customer

Asked: 2020-05-09

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-05-09

Question

Will EK0527 react with human samples?

Verified Customer

Verified customer

Asked: 2020-03-10

Answer

EK0527 does not react with human samples.

Boster Scientific Support

Answered: 2020-03-10

Question

Q: can I use citrate plasma as samples in Mouse TNF-alpha Picokine® ELISA Kit (Catalog # EK0527)?

W. Gonzalez

Verified customer

Asked: 2019-12-03

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of TNF-alpha causing degradation of its protein structure. TNF-alpha may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the TNF-alpha ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-12-03

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-11-21

Question

Q: What is the optimal O.D. value for TNF-alpha ELISA kit? I performed your TNF-alpha ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TNF-alpha even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TNF-alpha ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of TNF-alpha that can be declared/interpreted as positive by the above standard is 1pg/ml.

Boster Scientific Support

Answered: 2019-09-26

Question

Can you confirm if the EK0527 is compatible with ELISA microplate washers?

Verified customer

Asked: 2019-05-30

Answer

Our ELISA kits should be compatible for all ELISA microplate washers as long as it supports a 96 well plate.

Boster Scientific Support

Answered: 2019-05-30

Question

Can the sample diluent buffer, ABC diluent buffer and antibody diluent buffer be used interchangeably between the kit?

Verified customer

Asked: 2019-05-28

Answer

For the Mouse TNF Alpha ELISA Kit PicoKine™ (EK0527), the sample diluent buffer, ABC diluent buffer and antibody diluent buffer can be used interchangeably between the kit. However, the sample diluent buffer can only be used to dilute standards and samples, ABC diluent buffer is used to dilute ABC only and antibody diluent buffer is used to dilute antibody only.

Boster Scientific Support

Answered: 2019-05-29

Question

Is it possible for EK0527 to detect 0.5pg/ml concentrations of Mouse TNF Alpha? Do you have recommendations for a more sensitive kit?

Verified customer

Asked: 2019-05-06

Answer

Unfortunately, it would be difficult for Mouse TNF Alpha ELISA Kit PicoKine™ (EK0527) to detect that concentration and we are unable to offer a more sensitive kit.

Boster Scientific Support

Answered: 2019-05-07

Question

Q: Can TNF-alpha ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-02-06

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-02-06

Question

I would like to ask at what concentration of Mouse TNF alpha is measured in normal mouse plasma samples? As I research in literature the values indicates 10-40 pg/mL but your product EK0527 Mouse TNF Alpha ELISA Kit shows <1 pg/mL. Can this still detect my samples? As to not waste any of my plasma.

Verified Customer

Verified customer

Asked: 2019-01-24

Answer

Yes, it can detect your samples and we would recommend the starting dilution factor is 2-fold to reduce the risk of matrix effects. So, there is a wide range of possible detected levels, and some troubleshooting and optimization may be necessary if the samples contain a low level of the analytes.

Boster Scientific Support

Answered: 2019-01-24

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

H. Patel

Verified customer

Asked: 2018-06-27

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-06-27

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-03-06

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-03-06

Question

Q: which procedure should I follow in order to thaw whole blood sample for TNF-alpha ELISA after freezing?

Verified Customer

Verified customer

Asked: 2017-11-06

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TNF-alpha for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2017-11-06

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