|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-B Raf Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase B-raf(BRAF) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-B Raf Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9164)|
|Replacement Item||This antibody may replace the following items: sc-365410|sc-28988|sc-365610|sc-19886|sc-365409|sc-32774|sc-19889|sc-19887|sc-28989 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||E.coli-derived human B Raf recombinant protein (Position: A38-V230). Human B Raf shares 81% amino acid (aa) sequence identity with mouse B Raf.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Serine/threonine-protein kinase B-raf|
|Molecular Weight||84437 MW|
|Protein Function||Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. .|
|Tissue Specificity||Brain and testis.|
|Sequence Similarities||Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.|
|Subcellular Localization||Nucleus . Cytoplasm. Cell membrane . Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. .|
|Alternative Names||Serine/threonine-protein kinase B-raf;18.104.22.168;Proto-oncogene B-Raf;p94;v-Raf murine sarcoma viral oncogene homolog B1;BRAF;BRAF1, RAFB1;|
Background for Serine/threonine-protein kinase B-raf
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-B Raf Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-B Raf Picoband™ Antibody Images
Click the images to enlarge.
WB: Recombinant Human B Raf Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Lane 1: Rat Testis Tissue at 50ug
Lane 2: Rat Brain Tissue at 50ug
Lane 3: Mouse Testis Tissue at 50ug
Lane 4: Mouse Brain Tissue at 50ug
Lane 5: Hela Whole Cell Lysate at 40ug
Lane 6: Jurkat Whole Cell Lysate at 40ug
Lane 7: MCF-7 Whole Cell Lysate at 40ug
Lane 8: K562 Whole Cell Lysate at 40ug
Predicted bind size: 84KD
Observed bind size: 84KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,