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Product Info Summary
| SKU: | PA2112 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-RUNX3 Antibody Picoband®
SKU/Catalog Number
PA2112
BA3445-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RUNX3 Antibody catalog # PA2112. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RUNX3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2112)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse RUNX3, identical to the related rat sequence.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2112 is reactive to Runx3 in Mouse, Rat
Observed Molecular Weight
44 kDa
Calculated molecular weight
43.5 kDa
Background of Runx3
Runt-related transcription factor 3, also called AML2 is a protein that in humans is encoded by the RUNX3 gene. The RUNX3 gene encodes a Runt-related transcription factor, which is part of the RUNX gene family. By fluorescence in situ hybridization, RUNX3 was assigned to human chromosome 1p36.11. RUNX3 binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. It may be involved in the control of cellular proliferation and/or differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2112 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: Rat Liver Tissue, Mouse Liver Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-RUNX3 antibody, PA2112, Western blotting
All lanes: Anti RUNX3 (PA2112) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Predicted bind size: 44KD
Observed bind size: 44KD
Specific Publications For Anti-RUNX3 Antibody Picoband® (PA2112)
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Customer Q&As
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16 Customer Q&As for Anti-RUNX3 Antibody Picoband®
Question
I was wanting to use your anti-RUNX3 antibody for WB for mouse skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse skin identification?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
As indicated on the product datasheet, PA2112 anti-RUNX3 antibody has been tested for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-01
Question
Our lab want to know about using your anti-RUNX3 antibody for response to transforming growth factor beta studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
We appreciate your inquiry. This PA2112 anti-RUNX3 antibody is validated on rat liver tissue, tissue lysate, mouse liver tissue. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-23
Question
Here is the WB image, lot number and protocol we used for skin using anti-RUNX3 antibody PA2112. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-12
Question
My question regarding product PA2112, anti-RUNX3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2112 anti-RUNX3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-27
Question
Will PA2112 anti-RUNX3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
As indicated on the product datasheet, PA2112 anti-RUNX3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-22
Question
Is this PA2112 anti-RUNX3 antibody reactive to the isotypes of RUNX3?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
The immunogen of PA2112 anti-RUNX3 antibody is A synthetic peptide corresponding to a sequence in the middle region of mouse RUNX3(186-205aa QKIEDQTKAFPDRFGDLRMR), identical to the related rat sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-01
Question
Is a blocking peptide available for product anti-RUNX3 antibody (PA2112)?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
We do provide the blocking peptide for product anti-RUNX3 antibody (PA2112). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-24
Question
Our team were satisfied with the WB result of your anti-RUNX3 antibody. However we have been able to see positive staining in skin nucleus using this antibody. Is that expected? Could you tell me where is RUNX3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
Based on literature, skin does express RUNX3. Generally RUNX3 expresses in nucleus. Regarding which tissues have RUNX3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-06-18
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-RUNX3 antibody PA2112. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-10
Question
We are interested in to test anti-RUNX3 antibody PA2112 on mouse skin for research purposes, then I may be interested in using anti-RUNX3 antibody PA2112 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-02-28
Answer
The products we sell, including anti-RUNX3 antibody PA2112, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-28
Question
We are currently using anti-RUNX3 antibody PA2112 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on goat tissues as well?
C. Zhang
Verified customer
Asked: 2016-06-02
Answer
The anti-RUNX3 antibody (PA2112) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-06-02
Question
Will anti-RUNX3 antibody PA2112 work on goat WB with skin?
A. Thomas
Verified customer
Asked: 2016-02-24
Answer
Our lab technicians have not validated anti-RUNX3 antibody PA2112 on goat. You can run a BLAST between goat and the immunogen sequence of anti-RUNX3 antibody PA2112 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat skin in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-02-24
Question
We have been able to see staining in mouse cervix carcinoma. What should we do? Is anti-RUNX3 antibody supposed to stain cervix carcinoma positively?
B. Li
Verified customer
Asked: 2015-12-15
Answer
From what I have seen in literature cervix carcinoma does express RUNX3. From what I have seen in Uniprot.org, RUNX3 is expressed in tonsil, skin, cervix carcinoma, among other tissues. Regarding which tissues have RUNX3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-12-15
Question
Is there a BSA free version of anti-RUNX3 antibody PA2112 available?
L. Carter
Verified customer
Asked: 2015-11-19
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RUNX3 antibody PA2112 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-11-19
Question
Does anti-RUNX3 antibody PA2112 work for WB with skin?
C. Moore
Verified customer
Asked: 2014-05-12
Answer
According to the expression profile of skin, RUNX3 is highly expressed in skin. So, it is likely that anti-RUNX3 antibody PA2112 will work for WB with skin.
Boster Scientific Support
Answered: 2014-05-12
Question
I see that the anti-RUNX3 antibody PA2112 works with WB, what is the protocol used to produce the result images on the product page?
Z. Lewis
Verified customer
Asked: 2013-06-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-06-28
