Product Info Summary
SKU: | M00450-2 |
---|---|
Size: | 100ul |
Reactive Species: | Bovine, Human, Mouse, Rabbit, Rat, Swine |
Host: | Mouse |
Application: | IF, WB |
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Product info
Product Name
Anti-B23 NPM1 Monoclonal Antibody
SKU/Catalog Number
M00450-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-B23 NPM1 Monoclonal Antibody catalog # M00450-2. Tested in IF, WB applications. This antibody reacts with Bovine, Human, Mouse, Rabbit, Rat, Swine.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-B23 NPM1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00450-2)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Clone Number
9H1
Isotype
IgG
Immunogen
Purified recombinant human B23 protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00450-2 is reactive to NPM1 in Bovine, Human, Mouse, Rabbit, Rat, Swine
Applications
M00450-2 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
32.575kDa
Background of NPM1
Paired box protein Pax-6 also known as aniridia type II protein (AN2) or oculorhombin is a transcription factor with key functions in the development of the eye, nose, central nervous system and pancreas. Pax6 further seems to be required for the differentiation of pancreatic islet alpha cells. PAX6 has been shown to bind to a promoter element in the glucagon, insulin and somatostatin promoters and regulates the specification of the ventral neuron subtypes by establishing the correct progenitor domains. Isoform 5a appears to function as a molecular switch that specifies target genes. Anti-PAX6 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IF 1:100-1:500
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunofluorescent staining data of NPM1 using Anti-B23 NPM1 Monoclonal Antibody (M00450-2).
Immunofluorescence (IF) analysis of HeLa cells using B23 Monoclonal Antibody.Click image to see more details
Figure 1. Western blotting validation for Anti-B23 NPM1 Monoclonal Antibody M00450-2
Western Blot (WB) analysis using B23 Monoclonal Antibody against HeLaProtein Target Info & Infographic
Gene/Protein Information For NPM1 (Source: Uniprot.org, NCBI)
Gene Name
NPM1
Full Name
Nucleophosmin
Weight
32.575kDa
Superfamily
nucleoplasmin family
Alternative Names
B23; MGC104254; NPM1; NPMnucleophosmin/nucleoplasmin family, member 1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; nucleophosmin (nucleolar phosphoprotein B23, numatrin); Nucleophosmin; Numatrin NPM1 B23, NPM nucleophosmin 1 nucleophosmin|nucleolar protein NO38|nucleophosmin (nucleolar phosphoprotein B23, numatrin)|nucleophosmin/nucleoplasmin family, member 1|testicular tissue protein Li 128
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NPM1, check out the NPM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NPM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-B23 NPM1 Monoclonal Antibody (M00450-2)
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No publications found for M00450-2
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