Anti-Beclin 2 BECN2 Antibody

Product Name

Anti-Beclin 2 BECN2 Antibody

View all BECN1P1 Antibodies

SKU/Catalog Number

A12290

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Beclin 2 BECN2 Antibody (Catalog # A12290). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

Beclin 2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Beclin 2 BECN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12290)

Host

Rabbit

Contents

Beclin 2 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Beclin 2 antibody was raised against a 16 amino acid peptide near the amino terminus of human Beclin 2. The immunogen is located within amino acids 30 - 80 of Beclin 2.

Cross-reactivity

Beclin 2 antibody is human specific. At least two isoforms of Beclin 2 are known to exist; this antibody will only detect the longer isoform. Beclin 2 antibody is predicted to not cross-react with Beclin 1.

Reactive Species

A12290 is reactive to BECN1P1 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

48.2 kDa

Background of BECN1P1

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells (1,2). Beclin 2 is a mammalian specific homolog of the autophagy protein Beclin 1 (3). Like Beclin 1, Beclin 2 interacts with Bcl-2 and class III PI3K complex components. However, Beclin 2 functions in an additional lysosomal degradation pathway and is required for ligand-induced endolysosomal degradation of several G protein-coupled receptors (3). Beclin 2 is also required for agonist-induced lysosome-mediated degradation of EGFR in lung cancer cells, suggesting that it may also play a role in regulating other intracellular signaling pathways (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A12290 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Beclin 2 antibody can be used for detection of Beclin 2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information BECN1P1

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