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Product Info Summary
| SKU: | A09287-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-BAL/SLC27A5 Antibody Picoband®
SKU/Catalog Number
A09287-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BAL/SLC27A5 Antibody Picoband® catalog # A09287-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-BAL/SLC27A5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09287-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human BAL/SLC27A5 recombinant protein (Position: I204-L690).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09287-2 is reactive to SLC27A5 in Human, Rat
Observed Molecular Weight
75 kDa
Calculated molecular weight
75.4 kDa
Background of SLC27A5
Bile acyl-CoA synthetase is an enzyme that in humans is encoded by the SLC27A5 gene. The protein encoded by this gene is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty-acids containing 24- and 26-carbons. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. Its primary role is in fatty acid elongation or complex lipid synthesis rather than in degradation. This gene has a mouse ortholog.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09287-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human hepatocellular carcinoma paracancerous tissue (HCCP), rat liver tissue
FCM: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of BAL/SLC27A5 using anti-BAL/SLC27A5 antibody (A09287-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
Lane 2: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAL/SLC27A5 antigen affinity purified polyclonal antibody (Catalog # A09287-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAL/SLC27A5 at approximately 75 kDa. The expected band size for BAL/SLC27A5 is at 75 kDa.
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Flow Cytometry analysis of Jurkat cells using anti-BAL/SLC27A5 antibody (A09287-2).
Overlay histogram showing Jurkat cells stained with A09287-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAL/SLC27A5 Antibody (A09287-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-BAL/SLC27A5 Antibody Picoband® (A09287-2)
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