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- Table of Contents
Bile acyl-CoA synthetase Antibodies
AND SLC27A5 ELISA kits, Bile acyl-CoA synthetase Proteins
Many biological assays use antibodies to detect Bile acyl-CoA synthetase, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Bile acyl-CoA synthetase in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Bile acyl-CoA synthetase antibodies...
Designing an IHC experiment for Bile acyl-CoA synthetase? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the SLC27A5 IHC GuideSLC27A5 Infographic by Boster Bio
All SLC27A5 Products
Product
Figures
Specificity
Applications
Price
Human, Rat
ELISA, Flow Cytometry, WB
$370
Cat # A09287-2
100 μg/vial
Cat # A09287-2
100 μg/vial
SLC27A5 Overview
Facts about Bile acyl-CoA synthetase.
Bile acyl-CoA synthetase (SLC27A5)
Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the initial step in the conjugation of C24 bile acids (choloneates) into glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters.
Seems to trigger secondary bile acids entering the liver in the enterohepatic circulation. In vitro, also activates 3-alpha,7- alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving in the de novo synthesis from cholesterol.
Gene Information
| Human | |
|---|---|
| Gene Name: | SLC27A5 |
| Uniprot: | Q9Y2P5 |
| Entrez: | 10998 |
Family
| Belongs to: |
|---|
| ATP-dependent AMP-binding enzyme family |
Alternative Names
ACSB; ACSBBACS; ACSVL6; ACSVL6BAL; BA-CoA ligase; Bile acid-CoA ligase; bile acyl-CoA synthetase; Cholate--CoA ligase; EC 6.2.1.7; FACVL3; FACVL3FATP-5; FATP5; FATP5Solute carrier family 27 member 5; Fatty acid transport protein 5; Fatty-acid-coenzyme A ligase, very long-chain 3; FLJ22987; SLC27A5; solute carrier family 27 (fatty acid transporter), member 5; VLACSR; VLACSRVery long-chain acyl-CoA synthetase homolog 2; VLCSH2; VLCSH2Very long-chain acyl-CoA synthetase-related protein; VLCS-H2VLACS-related
Molecular Weight
Mass (kDA):
75.385 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 19q13.43 |
| Sequence: | 19; NC_000019.10 (58498333..58512065, complement) |
Tissue Specificity
Predominantly expressed in liver.
Subcellular Localizations
Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome. Cell membrane; Multi-pass membrane protein.
Diseases
- Fatty Liver
- Steatohepatitis
- Steatosis
- Liver Diseases
- Obesity
- Inborn Errors Of Metabolism
- Insulin Sensitivity
- Diabetes Mellitus
- Insulin Resistance
- Nonalcoholic Fatty Liver Disease
- Diabetes Mellitus, Non-insulin-dependent
- Icterus
Pathways
- Transport
- Fatty Acid Transport
- Bile Acid Conjugation
- Conjugation
- Lactation
- Long-chain Fatty Acid Transport
- Parturition
- Energy Homeostasis
- Localization
- Glucose Homeostasis
PTMs
- Amidation
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SLC27A5
bosterbio.com/bosterbio-gene-info-cards/SLC27A5
References
PMID: 10479480 by Steinberg S.J., et al. Human liver-specific very-long-chain acyl-coenzyme A synthetase: cDNA cloning and characterization of a second enzymatically active protein.
PMID: 10749848 by Steinberg S.J., et al. The human liver-specific homolog of very long-chain acyl-CoA synthetase is cholate:CoA ligase.