Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | A00432-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Type-2 angiotensin II receptor AGTR2 Antibody
SKU/Catalog Number
A00432-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Type-2 angiotensin II receptor AGTR2 Antibody (Catalog # A00432-1). Tested in ELISA, WB, IHC-P, ICC/IF, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
AGTR2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Type-2 angiotensin II receptor AGTR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00432-1)
Host
Rabbit
Contents
AGTR2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
AGTR2 antibody was raised against a 16 amino acid synthetic peptide from near the center of human AGTR2. The immunogen is located within amino acids 220 - 270 of AGTR2.
Reactive Species
A00432-1 is reactive to AGTR2 in Human, Mouse, Rat
Observed Molecular Weight
41 kDa
Calculated molecular weight
41.2 kDa
Background of AGTR2
Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion that acts through at least two types of receptors, AGTR1 and AGTR2. It is an important effector controlling blood pressure and volume in the cardiovascular system and plays a major role in the development of the mammalian kidney and urinary tract. Like AGTR1, AGTR2 is a seven transmembrane G protein-coupled receptor (GPCR), but AGTR2 does not demonstrate most of the classic features of GPCR signaling. AGTR2 is involved in a wide range of activities, including the induction of neurite outgrowth and the inhibition of cellular proliferation, in addition to the known function of mediation of vasoconstriction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00432-1 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-4 μg/mL; IHC-P: 1-4 μg/mL; IHC-P/IF: 10-20 μg/mL; ICC/IF: 5-20 μg/mL.
Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry in human and mouse samples; Immunofluorescence in human and rat samples. All other applications and species not yet tested.
Validation Images & Assay Conditions
Click image to see more details
WB Validation in Mouse Tissues Loading: 10 μg of lysate Antibodies: AGTR2, A00432-1, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
WB Validation in Rat Heart Loading: 15 μg of lysate Antibodies: AGTR2, A00432-1, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Immunohistochemistry Validation of AGTR2 in Human Lung Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-AGTR2 antibody (A00432-1) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Click image to see more details
Immunofluorescence Validation of AGTR2 in Jurkat Cells/strong> Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat Cells labeling AGTR2 with A00432-1 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Click image to see more details
Immunohistochemistry Validation of AGTR2 in Mouse Lung Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-AGTR2 antibody (A00432-1) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Specific Publications For Anti-Type-2 angiotensin II receptor AGTR2 Antibody (A00432-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Type-2 angiotensin II receptor AGTR2 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Type-2 angiotensin II receptor AGTR2 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
