Product Info Summary
SKU: | PA1870 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-BCRP/ABCG2 Antibody
View all ABCG2/CD338 Antibodies
SKU/Catalog Number
PA1870
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BCRP/ABCG2 Antibody catalog # PA1870. Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BCRP/ABCG2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1870)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse BCRP/ABCG2, different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1870 is reactive to ABCG2 in Mouse, Rat
Applications
PA1870 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
72.314kDa
Background of ABCG2/CD338
ABCG2 (Atp-binding cassette, subfamily g, member 2) also known as ABCP, BCRP or MRX, is a protein that in humans is encoded by the ABCG2 gene. The ABCG2 gene encodes a membrane transporter belonging to the ATP-binding cassette (ABC) superfamily of membrane transporters, which are involved in the trafficking of biologic molecules across cell membranes. The ABCG2 protein is also a high capacity transporter for uric acid excretion in the kidney, liver, and gut. The ABCG2 gene is mapped on 4q22.1. In vitro assays of isolated membrane preparations revealed a high-capacity, vanadate-sensitive ATPase activity associated with ABCG2 expression that was stimulated by compounds known to be transported by this protein. Ozvegy et al. (2001) concluded that ABCG2 is likely functioning as a homodimer or homooligomer in this expression system since it is unlikely that putative Sf9 transport partners would be overexpressed at similarly high levels.Abcg2 transports pheophorbide-a, which occurs in various plant-derived foods and food supplements and is highly efficient in limiting its uptake from ingested food. ABCG2 is a major factor in the concentrative transfer of drugs, carcinogens, and dietary toxins to the milk of mice, cows, and humans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-BCRP/ABCG2 antibody, PA1870, Western blotting
All lanes: Anti BCRP/ABCG2 (PA1870) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: Rat Kidney Tissue Lysate at 50ug
Lane 4: Mouse Spleen Tissue Lysate at 50ug
Predicted bind size: 72KD
Observed bind size: 72KD
Protein Target Info & Infographic
Gene/Protein Information For ABCG2 (Source: Uniprot.org, NCBI)
Gene Name
ABCG2
Full Name
Broad substrate specificity ATP-binding cassette transporter ABCG2
Weight
72.314kDa
Superfamily
ABC transporter superfamily
Alternative Names
ABC transporter; ABC15; ABCG2; ABCP; ABCPMGC102821; ATP-binding cassette sub-family G member 2; ATP-binding cassette transporter G2; ATP-binding cassette, sub-family G (WHITE), member 2; BCRP; BCRP1; BCRPMRX; BMDP; Breast cancer resistance protein; CD338 antigen; CD338; CDw338; EST157481; mitoxantrone resistance protein; Mitoxantrone resistance-associated protein; MXR; MXRMXR1; placenta specific MDR protein; Placenta-specific ATP-binding cassette transporter ABCG2 ABC15, ABCP, BCRP, BCRP1, BMDP, CD338, CDw338, EST157481, GOUT1, MRX, MXR, MXR-1, MXR1, UAQTL1 ATP binding cassette subfamily G member 2 (Junior blood group) broad substrate specificity ATP-binding cassette transporter ABCG2|ABC transporter|ATP-binding cassette transporter G2|ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group)|breast cancer resistance protein|mitoxantrone resistance-associated protein|multi drug resistance efflux transport ATP-binding cassette sub-family G (WHITE) member 2|placenta specific MDR protein|placenta-specific ATP-binding cassette transporter|urate exporter
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ABCG2, check out the ABCG2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ABCG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BCRP/ABCG2 Antibody (PA1870)
Hello CJ!
PA1870 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Characteristics of doxorubicin-selected multidrug-resistant human leukemia HL-60 cells with tolerance to arsenic trioxide and contribution of leukemia stem cells
Reversion of malignant phenotypes of human glioblastoma cells by ?-elemene through ?-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules
Wang Nn, Zhao Lj, Wu Ln, He Mf, Qu Jw, Zhao Yb, Zhao Wz, Li Js, Wang Jh. Asian Pac J Cancer Prev. 2013;14(9):4983-8. Mechanistic Analysis Of Taxol-Induced Multidrug Resistance In An Ovarian Cancer Cell Line.
Qiao B, Johnson Nw, Chen X, Li R, Tao Q, Gao J. Oncol Rep. 2011 Aug;26(2):455-61. Doi: 10.3892/Or.2011.1299. Epub 2011 May 10. Disclosure Of A Stem Cell Phenotype In An Oral Squamous Cell Carcinoma Cell Line Induced By Bmp-4 Via An Epithelial-Mese...
Duan J, Mansour Hm, Zhang Y, Deng X, Chen Y, Wang J, Pan Y, Zhao J. Int J Pharm. 2012 Apr 15;426(1-2):193-201. Doi: 10.1016/J.Ijpharm.2012.01.020. Epub 2012 Jan 17. Reversion Of Multidrug Resistance By Co-Encapsulation Of Doxorubicin And Curcumin ...
Li R, Wu X, Wei H, Tian S. Oncol Lett. 2013 Mar;5(3):877-883. Epub 2013 Jan 2. Characterization Of Side Population Cells Isolated From The Gastric Cancer Cell Line Sgc-7901.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Anti-BCRP/ABCG2 Antibody
Question
Will anti-BCRP/ABCG2 antibody PA1870 work for WB with pancreas pns?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
According to the expression profile of pancreas pns, ABCG2 is highly expressed in pancreas pns. So, it is likely that anti-BCRP/ABCG2 antibody PA1870 will work for WB with pancreas pns.
Boster Scientific Support
Answered: 2020-04-03
Question
I see that the anti-BCRP/ABCG2 antibody PA1870 works with WB, what is the protocol used to produce the result images on the product page?
F. Miller
Verified customer
Asked: 2020-01-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-03
Question
Does anti-BCRP/ABCG2 antibody PA1870 work on feline WB with hippocampus placenta?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
Our lab technicians have not tested anti-BCRP/ABCG2 antibody PA1870 on feline. You can run a BLAST between feline and the immunogen sequence of anti-BCRP/ABCG2 antibody PA1870 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline hippocampus placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-19
Question
My question regarding product PA1870, anti-BCRP/ABCG2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-11
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1870 anti-BCRP/ABCG2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-11
Question
Is this PA1870 anti-BCRP/ABCG2 antibody reactive to the isotypes of ABCG2?
S. Wu
Verified customer
Asked: 2015-08-03
Answer
The immunogen of PA1870 anti-BCRP/ABCG2 antibody is A synthetic peptide corresponding to a sequence in the middle region of mouse BCRP/ABCG2(152-167aa KNHEKNERINTIIKEL), different from the related rat sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-08-03
Question
We are currently using anti-BCRP/ABCG2 antibody PA1870 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on canine tissues as well?
A. Krishna
Verified customer
Asked: 2013-09-02
Answer
The anti-BCRP/ABCG2 antibody (PA1870) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-09-02