Anti-BCRP/ABCG2 Antibody

ABCG2/CD338 antibody

Boster Bio Anti-BCRP/ABCG2 Antibody catalog # PA1870. Tested in WB applications. This antibody reacts with Mouse, Rat. Cited in 6 publication(s).

Product Info Summary

SKU: PA1870
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-BCRP/ABCG2 Antibody

View all ABCG2/CD338 Antibodies

SKU/Catalog Number

PA1870

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BCRP/ABCG2 Antibody catalog # PA1870. Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BCRP/ABCG2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1870)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse BCRP/ABCG2, different from the related rat sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1870 is reactive to ABCG2 in Mouse, Rat

Applications

PA1870 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

72.314kDa

Background of ABCG2/CD338

ABCG2 (Atp-binding cassette, subfamily g, member 2) also known as ABCP, BCRP or MRX, is a protein that in humans is encoded by the ABCG2 gene. The ABCG2 gene encodes a membrane transporter belonging to the ATP-binding cassette (ABC) superfamily of membrane transporters, which are involved in the trafficking of biologic molecules across cell membranes. The ABCG2 protein is also a high capacity transporter for uric acid excretion in the kidney, liver, and gut. The ABCG2 gene is mapped on 4q22.1. In vitro assays of isolated membrane preparations revealed a high-capacity, vanadate-sensitive ATPase activity associated with ABCG2 expression that was stimulated by compounds known to be transported by this protein. Ozvegy et al. (2001) concluded that ABCG2 is likely functioning as a homodimer or homooligomer in this expression system since it is unlikely that putative Sf9 transport partners would be overexpressed at similarly high levels.Abcg2 transports pheophorbide-a, which occurs in various plant-derived foods and food supplements and is highly efficient in limiting its uptake from ingested food. ABCG2 is a major factor in the concentrative transfer of drugs, carcinogens, and dietary toxins to the milk of mice, cows, and humans.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For ABCG2 (Source: Uniprot.org, NCBI)

Gene Name

ABCG2

Full Name

Broad substrate specificity ATP-binding cassette transporter ABCG2

Weight

72.314kDa

Superfamily

ABC transporter superfamily

Alternative Names

ABC transporter; ABC15; ABCG2; ABCP; ABCPMGC102821; ATP-binding cassette sub-family G member 2; ATP-binding cassette transporter G2; ATP-binding cassette, sub-family G (WHITE), member 2; BCRP; BCRP1; BCRPMRX; BMDP; Breast cancer resistance protein; CD338 antigen; CD338; CDw338; EST157481; mitoxantrone resistance protein; Mitoxantrone resistance-associated protein; MXR; MXRMXR1; placenta specific MDR protein; Placenta-specific ATP-binding cassette transporter ABCG2 ABC15, ABCP, BCRP, BCRP1, BMDP, CD338, CDw338, EST157481, GOUT1, MRX, MXR, MXR-1, MXR1, UAQTL1 ATP binding cassette subfamily G member 2 (Junior blood group) broad substrate specificity ATP-binding cassette transporter ABCG2|ABC transporter|ATP-binding cassette transporter G2|ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group)|breast cancer resistance protein|mitoxantrone resistance-associated protein|multi drug resistance efflux transport ATP-binding cassette sub-family G (WHITE) member 2|placenta specific MDR protein|placenta-specific ATP-binding cassette transporter|urate exporter

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ABCG2, check out the ABCG2 Infographic

ABCG2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ABCG2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1870 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Characteristics of doxorubicin-selected multidrug-resistant human leukemia HL-60 cells with tolerance to arsenic trioxide and contribution of leukemia stem cells

Reversion of malignant phenotypes of human glioblastoma cells by ?-elemene through ?-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules

Wang Nn, Zhao Lj, Wu Ln, He Mf, Qu Jw, Zhao Yb, Zhao Wz, Li Js, Wang Jh. Asian Pac J Cancer Prev. 2013;14(9):4983-8. Mechanistic Analysis Of Taxol-Induced Multidrug Resistance In An Ovarian Cancer Cell Line.

Qiao B, Johnson Nw, Chen X, Li R, Tao Q, Gao J. Oncol Rep. 2011 Aug;26(2):455-61. Doi: 10.3892/Or.2011.1299. Epub 2011 May 10. Disclosure Of A Stem Cell Phenotype In An Oral Squamous Cell Carcinoma Cell Line Induced By Bmp-4 Via An Epithelial-Mese...

Duan J, Mansour Hm, Zhang Y, Deng X, Chen Y, Wang J, Pan Y, Zhao J. Int J Pharm. 2012 Apr 15;426(1-2):193-201. Doi: 10.1016/J.Ijpharm.2012.01.020. Epub 2012 Jan 17. Reversion Of Multidrug Resistance By Co-Encapsulation Of Doxorubicin And Curcumin ...

Li R, Wu X, Wei H, Tian S. Oncol Lett. 2013 Mar;5(3):877-883. Epub 2013 Jan 2. Characterization Of Side Population Cells Isolated From The Gastric Cancer Cell Line Sgc-7901.

Have you used Anti-BCRP/ABCG2 Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-BCRP/ABCG2 Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

6 Customer Q&As for Anti-BCRP/ABCG2 Antibody

Question

Will anti-BCRP/ABCG2 antibody PA1870 work for WB with pancreas pns?

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

According to the expression profile of pancreas pns, ABCG2 is highly expressed in pancreas pns. So, it is likely that anti-BCRP/ABCG2 antibody PA1870 will work for WB with pancreas pns.

Boster Scientific Support

Answered: 2020-04-03

Question

I see that the anti-BCRP/ABCG2 antibody PA1870 works with WB, what is the protocol used to produce the result images on the product page?

F. Miller

Verified customer

Asked: 2020-01-03

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-01-03

Question

Does anti-BCRP/ABCG2 antibody PA1870 work on feline WB with hippocampus placenta?

Verified Customer

Verified customer

Asked: 2019-07-19

Answer

Our lab technicians have not tested anti-BCRP/ABCG2 antibody PA1870 on feline. You can run a BLAST between feline and the immunogen sequence of anti-BCRP/ABCG2 antibody PA1870 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline hippocampus placenta in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-19

Question

My question regarding product PA1870, anti-BCRP/ABCG2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-12-11

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1870 anti-BCRP/ABCG2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-12-11

Question

Is this PA1870 anti-BCRP/ABCG2 antibody reactive to the isotypes of ABCG2?

S. Wu

Verified customer

Asked: 2015-08-03

Answer

The immunogen of PA1870 anti-BCRP/ABCG2 antibody is A synthetic peptide corresponding to a sequence in the middle region of mouse BCRP/ABCG2(152-167aa KNHEKNERINTIIKEL), different from the related rat sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2015-08-03

Question

We are currently using anti-BCRP/ABCG2 antibody PA1870 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on canine tissues as well?

A. Krishna

Verified customer

Asked: 2013-09-02

Answer

The anti-BCRP/ABCG2 antibody (PA1870) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-09-02

Size

Conjugation

Total: $370

SKU:PA1870

In stock, 3+ left.

Order within 30 hours and 57 minutes to receive by Thu Mar 21

Get A Quote
Eddy test
In stock
Order Product
PA1870
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.