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Product Info Summary
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Boster Bio Anti-BCRP/ABCG2 Antibody catalog # PA1869. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BCRP/ABCG2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1869)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence in the middle region of human BCRP/ABCG2 (154-168aa NHEKNERINRVIQEL).
No cross reactivity with other proteins
PA1869 is reactive to ABCG2 in Human
PA1869 is guaranteed for WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-BCRP/ABCG2 antibody, PA1869, Western blotting
All lanes: Anti BCRP/ABCG2 (PA1869) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 72KD
Observed bind size: 72KD
Gene/Protein Information For ABCG2 (Source: Uniprot.Org, NCBI)
Broad substrate specificity ATP-binding cassette transporter ABCG2
ABC transporter superfamily
ABC transporter; ABC15; ABCG2; ABCP; ABCPMGC102821; ATP-binding cassette sub-family G member 2; ATP-binding cassette transporter G2; ATP-binding cassette, sub-family G (WHITE), member 2; BCRP; BCRP1; BCRPMRX; BMDP; Breast cancer resistance protein; CD338 antigen; CD338; CDw338; EST157481; mitoxantrone resistance protein; Mitoxantrone resistance-associated protein; MXR; MXRMXR1; placenta specific MDR protein; Placenta-specific ATP-binding cassette transporter ABCG2 ABC15, ABCP, BCRP, BCRP1, BMDP, CD338, CDw338, EST157481, GOUT1, MRX, MXR, MXR-1, MXR1, UAQTL1 ATP binding cassette subfamily G member 2 (Junior blood group) broad substrate specificity ATP-binding cassette transporter ABCG2|ABC transporter|ATP-binding cassette transporter G2|ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group)|breast cancer resistance protein|mitoxantrone resistance-associated protein|multi drug resistance efflux transport ATP-binding cassette sub-family G (WHITE) member 2|placenta specific MDR protein|placenta-specific ATP-binding cassette transporter|urate exporter*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ABCG2, check out the ABCG2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ABCG2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PA1869 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Characteristics of doxorubicin-selected multidrug-resistant human leukemia HL-60 cells with tolerance to arsenic trioxide and contribution of leukemia stem cells
Reversion of malignant phenotypes of human glioblastoma cells by ?-elemene through ?-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules
Wang Nn, Zhao Lj, Wu Ln, He Mf, Qu Jw, Zhao Yb, Zhao Wz, Li Js, Wang Jh. Asian Pac J Cancer Prev. 2013;14(9):4983-8. Mechanistic Analysis Of Taxol-Induced Multidrug Resistance In An Ovarian Cancer Cell Line.
Qiao B, Johnson Nw, Chen X, Li R, Tao Q, Gao J. Oncol Rep. 2011 Aug;26(2):455-61. Doi: 10.3892/Or.2011.1299. Epub 2011 May 10. Disclosure Of A Stem Cell Phenotype In An Oral Squamous Cell Carcinoma Cell Line Induced By Bmp-4 Via An Epithelial-Mese...
Duan J, Mansour Hm, Zhang Y, Deng X, Chen Y, Wang J, Pan Y, Zhao J. Int J Pharm. 2012 Apr 15;426(1-2):193-201. Doi: 10.1016/J.Ijpharm.2012.01.020. Epub 2012 Jan 17. Reversion Of Multidrug Resistance By Co-Encapsulation Of Doxorubicin And Curcumin ...
Li R, Wu X, Wei H, Tian S. Oncol Lett. 2013 Mar;5(3):877-883. Epub 2013 Jan 2. Characterization Of Side Population Cells Isolated From The Gastric Cancer Cell Line Sgc-7901.
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