Click image to see more details
Product Info Summary
| SKU: | M00072 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-beta 2 Adrenergic Receptor ADRB2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00072
BM4957 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-beta 2 Adrenergic Receptor ADRB2 Rabbit Monoclonal Antibody catalog # M00072. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta 2 Adrenergic Receptor ADRB2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00072)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AE-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human beta 2 Adrenergic Receptor
Reactive Species
M00072 is reactive to ADRB2 in Human, Mouse, Rat
Observed Molecular Weight
62 kDa
Calculated molecular weight
46.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00072 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:30
Positive Control
WB: A431 cell
Validation Images & Assay Conditions
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Western blot analysis of beta 2 Adrenergic Receptor expression in A431 cell lysate.
Specific Publications For Anti-beta 2 Adrenergic Receptor ADRB2 Rabbit Monoclonal Antibody (M00072)
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Customer Q&As
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7 Customer Q&As for Anti-beta 2 Adrenergic Receptor ADRB2 Rabbit Monoclonal Antibody
Question
Is M00072 conjugated or unconjugated?
Verified customer
Asked: 2022-07-21
Answer
The Anti-Beta 2 Adrenergic Receptor ADRB2 Rabbit Monoclonal Antibody (M00072) is unconjugated.
Boster Scientific Support
Answered: 2022-07-22
Question
We purchased anti-beta 2 Adrenergic Receptor Rabbit Monoclonal antibody for IP on thyroid in a previous experiment. I am using human, and We intend to use the antibody for WB next. Our lab want to know about examining thyroid as well as squamous epithelium in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
I have checked the website and datasheets of our anti-beta 2 Adrenergic Receptor Rabbit Monoclonal antibody and it appears that M00072 has been tested on human in both IP and WB. Thus M00072 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-17
Question
We have been able to see staining in human thyroid. Are there any suggestions? Is anti-beta 2 Adrenergic Receptor Rabbit Monoclonal antibody supposed to stain thyroid positively?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
From what I have seen in literature thyroid does express ADRB2. From what I have seen in Uniprot.org, ADRB2 is expressed in squamous epithelium, brain, blood, heart, thyroid, fetal brain, leukocyte prostate, embryonic kidney, among other tissues. Regarding which tissues have ADRB2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 11246467
Brain, Pubmed ID: 3026848, 14702039
Embryonic kidney, Pubmed ID: 17525332
Fetal brain, Leukocyte, and Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-14
Question
I would like using your anti-beta 2 Adrenergic Receptor Rabbit Monoclonal antibody for desensitization of g protein-coupled receptor signaling pathway by arrestin studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
We appreciate your inquiry. This M00072 anti-beta 2 Adrenergic Receptor Rabbit Monoclonal antibody is validated on a431 cell lysate. It is guaranteed to work for IP, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-31
Question
My colleagues were happy with the WB result of your anti-beta 2 Adrenergic Receptor Rabbit Monoclonal antibody. However we have been able to see positive staining in fetal brain cell membrane using this antibody. Is that expected? Could you tell me where is ADRB2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
From literature, fetal brain does express ADRB2. Generally ADRB2 expresses in cell membrane. Regarding which tissues have ADRB2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 11246467
Brain, Pubmed ID: 3026848, 14702039
Embryonic kidney, Pubmed ID: 17525332
Fetal brain, Leukocyte, and Prostate, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-08-13
Question
We are currently using anti-beta 2 Adrenergic Receptor Rabbit Monoclonal antibody M00072 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-07
Answer
The anti-beta 2 Adrenergic Receptor Rabbit Monoclonal antibody (M00072) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-07
Question
Is this M00072 anti-beta 2 Adrenergic Receptor Rabbit Monoclonal antibody reactive to the isotypes of ADRB2?
B. Williams
Verified customer
Asked: 2016-05-06
Answer
The immunogen of M00072 anti-beta 2 Adrenergic Receptor Rabbit Monoclonal antibody is A synthesized peptide derived from human beta 2 Adrenergic Receptor. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-05-06
