Product Info Summary
SKU: | EKC2313 |
---|---|
Size: | 1 kit, containing two 96-well plates and all necessary reagents |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | Cell lines |
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Product info
Product Name
Adrenergic Receptor beta2 (Phospho-Ser346) Colorimetric Cell-Based ELISA Kit
See all beta-2 Adrenergic R/ADRB2 products
SKU/Catalog Number
EKC2313
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The Adrenergic Receptor beta2 (Phospho-Ser346) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Adrenergic Receptor beta2 (Phospho-Ser346) protein expression profile in cells. The kit can be used for measuring the relative amounts of Adrenergic Receptor beta2 (Phospho-Ser346) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Adrenergic Receptor beta2 (Phospho-Ser346).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
Adrenergic Receptor beta2 (Phospho-Ser346) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2313)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2313 is reactive to ADRB2 in Human samples
Validated Sample Types
Cell lines
Application Guarantee
EKC2313 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Reagent | Quantity | Container |
---|---|---|
96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
10x TBS | 24 ml (10x) | Clear |
Quenching Buffer | 24 ml (1x) | Clear |
Blocking Buffer | 50 ml (1x) | Clear |
15x Wash Buffer | 50 ml (15x) | Clear |
100x Anti-ADRB2 (Phospho-specific) Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
100x Anti-ADRB2 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
Primary Antibody Diluent | 12 ml (1x) | Clear |
Ready-to-Use Substrate | 12 ml (1x) | Brown |
Stop Solution | 12 ml (1x) | Clear |
Crystal Violet Solution | 12ml (1x) | Glass |
SDS Solution | 24 ml (1x) | Clear |
Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from HepG2 cells treated with nocodazole 1ug/ml 16h
Protein Target Info & Infographic
Gene/Protein Information For ADRB2 (Source: Uniprot.Org, NCBI)
Gene Name
ADRB2
Full Name
Beta-2 adrenergic receptor
Weight
46.459kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
ADRB2; ADRB2R; ADRBR; adrenergic, beta-2-, receptor, surface; B2AR; B2ARADRB2R; BAR; beta-2 Adrenergic R; beta-2 adrenergic receptor; beta2 AdrenergicR; beta-2 AdrenergicR; Beta-2 adrenoceptor; Beta-2 adrenoreceptor; BETA2AR; catecholamine receptor ADRB2 ADRB2R, ADRBR, B2AR, BAR, BETA2AR adrenoceptor beta 2 beta-2 adrenergic receptor|adrenergic, beta-2-, receptor, surface|adrenoceptor beta 2 surface|beta-2 adrenoceptor|beta-2 adrenoreceptor|catecholamine receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ADRB2, check out the ADRB2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ADRB2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Adrenergic Receptor beta2 (Phospho-Ser346) Colorimetric Cell-Based ELISA Kit (EKC2313)
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