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Product Info Summary
| SKU: | M00730-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-BID Mouse Monoclonal Antibody [Clone ID: OTI3E9]
SKU/Catalog Number
M00730-2
Size
100 µl
Description
Boster Bio BID mouse monoclonal antibody, clone OTI3E9 (formerly 3E9). Catalog# M00730-2. Tested in IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BID Mouse Monoclonal Antibody [Clone ID: OTI3E9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00730-2)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3E9
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human BID (NP_001187) produced in HEK293T cell.
Reactive Species
M00730-2 is reactive to BID in Human, Monkey, Mouse
Calculated molecular weight
22.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00730-2 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:400~4000
IHC 1:150
IF 1:100
Validation Images & Assay Conditions
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Anti-BID mouse monoclonal antibody (M00730-2) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BID.
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Immunofluorescent staining of HeLa cells using anti-BID mouse monoclonal antibody (M00730-2).
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BID (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BID.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BID monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Specific Publications For Anti-BID Mouse Monoclonal Antibody [Clone ID: OTI3E9] (M00730-2)
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