Anti-Bid Antibody Picoband™ (monoclonal, 10F4)

BID antibody

Boster Bio Anti-Bid Antibody Picoband™ (monoclonal, 10F4) catalog # M00730-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M00730-1
Size: 100 μg/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Bid Antibody Picoband™ (monoclonal, 10F4)

View all BID Antibodies

SKU/Catalog Number

M00730-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Bid Antibody Picoband™ (monoclonal, 10F4) catalog # M00730-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Bid Antibody Picoband™ (monoclonal, 10F4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00730-1)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

10F4

Isotype

Mouse IgG2b

Immunogen

E.coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequences identity with mouse and rat Bid, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M00730-1 is reactive to BID in Human

Applications

M00730-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

22 kDa

Calculated molecular weight

21.995kDa

Background of BID

BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al. (1998) mapped the human BID gene to 22q11. Luo et al. (1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For BID (Source: Uniprot.org, NCBI)

Gene Name

BID

Full Name

BH3-interacting domain death agonist

Weight

21.995kDa

Alternative Names

apoptic death agonist; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; BID; desmocollin type 4; FP497; Human BID coding sequence; MGC15319; MGC42355; p22 BID BID FP497 BH3 interacting domain death agonist BH3-interacting domain death agonist|Human BID coding sequence|apoptic death agonist|desmocollin type 4|p22 BID

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BID, check out the BID Infographic

BID infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BID: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M00730-1

100μg

$370
M00730-1-10ug

10μg sample (liquid)

$99
M00730-1-Biotin

100 μg Biotin conjugated

$570
M00730-1-Cy3

100 μg Cy3 conjugated

$570
M00730-1-Dylight488

100 μg Dylight488 conjugated

$570
M00730-1-Dylight550

100 μg Dylight550 conjugated

$570
M00730-1-Dylight594

100 μg Dylight594 conjugated

$570
M00730-1-FITC

100 μg FITC conjugated

$570
M00730-1-HRP

100 μg HRP conjugated

$570
M00730-1-APC

100 μg APC conjugated

$670
M00730-1-PE

100 μg PE conjugated

$670
M00730-1-carrier-free

Carrier Free

$370

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M00730-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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