Anti-Bid Antibody Picoband™

Boster Bio Anti-Bid Antibody Picoband™ catalog # A00730. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: A00730
Size: 100ug/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Bid Antibody Picoband™

See all Bid products

SKU/Catalog Number

A00730

Size

100ug/vial

Form

Lyophilized

Description

Boster Bio Anti-Bid Antibody Picoband™ catalog # A00730. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Bid Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00730)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived mouse Bid recombinant protein (Position: M1-D195). Mouse Bid shares 64.6% and 87.2% amino acid (aa) sequence identity with human and rat Bid, respectively.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00730 is reactive to Bid in Mouse, Rat

Applications

A00730 is guaranteed for IHC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat Western blot, 0.1-0.5μg/ml, Mouse,

Validation Images & Assay Conditions

Gene/Protein Information For Bid (Source: Uniprot.Org, NCBI)

Uniprot ID

P70444

Gene ID

12122

Gene Name

Bid

Full Name

BH3-interacting domain death agonist

Weight

21.952kDa

Alternative Names

apoptic death agonist; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; BID; desmocollin type 4; FP497; Human BID coding sequence; MGC15319; MGC42355; p22 BID Bid|2700049M22Rik, AI875481, AU022477|BH3 interacting domain death agonist|BH3-interacting domain death agonist|p22 BID

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Bid, check out the Bid Infographic

Bid infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Bid: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A00730

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Bid Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Bid Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

16 Customer Q&As for Anti-Bid Antibody Picoband™

Question

Is this A00730 anti-Bid antibody reactive to the isotypes of BID?

Verified Customer

Verified customer

Asked: 2020-03-25

Answer

The immunogen of A00730 anti-Bid antibody is E.coli-derived mouse Bid recombinant protein (Position: M1-D195). Mouse Bid shares 64.6% and 87.2% amino acid (aa) sequence identity with human and rat Bid, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-25

Question

We have seen staining in rat embryonic kidney. Are there any suggestions? Is anti-Bid antibody supposed to stain embryonic kidney positively?

Verified Customer

Verified customer

Asked: 2020-03-09

Answer

According to literature embryonic kidney does express BID. According to Uniprot.org, BID is expressed in blood, brain skin, embryonic kidney, liver, among other tissues. Regarding which tissues have BID expression, here are a few articles citing expression in various tissues:
Brain, and Skin, Pubmed ID: 15489334
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2020-03-09

Question

Please see the WB image, lot number and protocol we used for embryonic kidney using anti-Bid antibody A00730. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-27

Question

I was wanting to use your anti-Bid antibody for WB for mouse embryonic kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse embryonic kidney identification?

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

As indicated on the product datasheet, A00730 anti-Bid antibody has been tested for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse embryonic kidney in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-11

Question

I see that the anti-Bid antibody A00730 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-02-03

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-02-03

Question

Do you have a BSA free version of anti-Bid antibody A00730 available?

Verified Customer

Verified customer

Asked: 2019-12-31

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bid antibody A00730 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-31

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-Bid antibody A00730. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-13

Question

Would anti-Bid antibody A00730 work for WB with embryonic kidney?

Verified Customer

Verified customer

Asked: 2019-12-12

Answer

According to the expression profile of embryonic kidney, BID is highly expressed in embryonic kidney. So, it is likely that anti-Bid antibody A00730 will work for WB with embryonic kidney.

Boster Scientific Support

Answered: 2019-12-12

Question

Is a blocking peptide available for product anti-Bid antibody (A00730)?

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

We do provide the blocking peptide for product anti-Bid antibody (A00730). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-06

Question

We bought anti-Bid antibody for WB on brain skin in the past. I am using rat, and We intend to use the antibody for IHC next. We want examining brain skin as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

I looked at the website and datasheets of our anti-Bid antibody and I see that A00730 has been validated on rat in both WB and IHC. Thus A00730 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-12-03

Question

Can you help my question with product A00730, anti-Bid antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00730 anti-Bid antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-21

Question

We are currently using anti-Bid antibody A00730 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-08

Answer

The anti-Bid antibody (A00730) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-08

Question

My lab would like using your anti-Bid antibody for apoptotic mitochondrial changes studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

B. Wu

Verified customer

Asked: 2018-06-21

Answer

I appreciate your inquiry. This A00730 anti-Bid antibody is tested on mouse spleen tissue, tissue lysate, rat spleen tissue, brain tissue. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-06-21

Question

Would A00730 anti-Bid antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

C. Gonzalez

Verified customer

Asked: 2018-01-23

Answer

It shows on the product datasheet, A00730 anti-Bid antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-01-23

Question

Our team were satisfied with the WB result of your anti-Bid antibody. However we have been able to see positive staining in embryonic kidney isoform 3: cytoplasm using this antibody. Is that expected? Could you tell me where is BID supposed to be expressed?

B. Anderson

Verified customer

Asked: 2015-06-18

Answer

Based on literature, embryonic kidney does express BID. Generally BID expresses in cytoplasm., bh3-interacting domain death agonist p15:, bh3-interacting domain death agonist p13:, isoform 1: cytoplasm, isoform 3: cytoplasm, isoform 2: mitochondrion membrane. Regarding which tissues have BID expression, here are a few articles citing expression in various tissues:
Brain, and Skin, Pubmed ID: 15489334
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2015-06-18

Question

I am interested in to test anti-Bid antibody A00730 on mouse embryonic kidney for research purposes, then I may be interested in using anti-Bid antibody A00730 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

O. Zhang

Verified customer

Asked: 2013-03-25

Answer

The products we sell, including anti-Bid antibody A00730, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-03-25

Conjugation

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
A00730
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.