Overview
| Product Name |
Anti-Bid Antibody Picoband™
See more Bid products |
| Catalog# |
A00730 |
| Pack Size |
100ug/vial |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-Bid Antibody Picoband™ catalog # A00730. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. Supplied as 100ug/vial in Lyophilized form antibody. |
| Cite This Product |
Anti-Bid Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00730)
|
| Antibodies Validation |
Antibodies Validation Information
|
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Anti-Bid Antibody Picoband™ may replace the following items: sc 34325|sc 34325 R|sc 11423|sc 6291|sc 6539|sc 6538|sc 373939|sc 56025|sc 135847|sc 514622. |
Product Specs
| Host |
Rabbit |
| Reactive Species |
Mouse, Rat |
| Applications |
IHC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $100. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
E.coli-derived mouse Bid recombinant protein (Position: M1-D195). Mouse Bid shares 64.6% and 87.2% amino acid (aa) sequence identity with human and rat Bid, respectively. |
| Cross Reactivity |
No cross reactivity with other proteins. |
Gene/Protein Basic Information For Bid (Source: Uniprot.org, NCBI)
| Uniprot Id | P70444 |
|---|
| NCBI Gene Id | 12122 |
|---|
| Species Of This Entry | Mouse |
|---|
| Gene Name | Bid |
|---|
| Protein Name | BH3-interacting domain death agonist |
|---|
| Alternative Names | Bid|apoptic death agonist; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; BID; desmocollin type 4; FP497; Human BID coding sequence; MGC15319; MGC42355; p22 BID |
|---|
| Molecular Weight | 21952 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Bid, check out the Bid Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Bid: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Bid infographic
Our Boster Quality Guarantee for Anti-Bid Antibody Picoband™ covers its use in the following applications.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse,
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Figure 2. IHC analysis of Bid using anti-Bid antibody (A00730).
Bid was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Bid Antibody (A00730) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of Bid using anti-Bid antibody (A00730).
Bid was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Bid Antibody (A00730) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1. Western blot analysis of Bcl-2 using anti-Bcl-2 antibody (A00730).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse brain tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl-2 antigen affinity purified polyclonal antibody (Catalog # A00730) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl-2 at approximately 22KD. The expected band size for Bcl-2 is at 22KD.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
Other Recommended Resources
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Questions and answers from customers related to A00730 Anti-Bid Antibody Picoband™
16 Related Questions
Question
Is this A00730 anti-Bid antibody reactive to the isotypes of BID?
Verified Customer
Asked: 2020-03-25
Answer
The immunogen of A00730 anti-Bid antibody is E.coli-derived mouse Bid recombinant protein (Position: M1-D195). Mouse Bid shares 64.6% and 87.2% amino acid (aa) sequence identity with human and rat Bid, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-25
Question
We have seen staining in rat embryonic kidney. Are there any suggestions? Is anti-Bid antibody supposed to stain embryonic kidney positively?
Verified Customer
Asked: 2020-03-09
Answer
According to literature embryonic kidney does express BID. According to Uniprot.org, BID is expressed in blood, brain skin, embryonic kidney, liver, among other tissues. Regarding which tissues have BID expression, here are a few articles citing expression in various tissues:
Brain, and Skin, Pubmed ID: 15489334
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-03-09
Question
Please see the WB image, lot number and protocol we used for embryonic kidney using anti-Bid antibody A00730. Please let me know if you require anything else.
Verified Customer
Asked: 2020-02-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-27
Question
I was wanting to use your anti-Bid antibody for WB for mouse embryonic kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse embryonic kidney identification?
Verified Customer
Asked: 2020-02-11
Answer
As indicated on the product datasheet, A00730 anti-Bid antibody has been tested for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-11
Question
I see that the anti-Bid antibody A00730 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Asked: 2020-02-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-03
Question
Do you have a BSA free version of anti-Bid antibody A00730 available?
Verified Customer
Asked: 2019-12-31
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bid antibody A00730 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-31
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-Bid antibody A00730. Let me know if you need anything else.
Verified Customer
Asked: 2019-12-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-13
Question
Would anti-Bid antibody A00730 work for WB with embryonic kidney?
Verified Customer
Asked: 2019-12-12
Answer
According to the expression profile of embryonic kidney, BID is highly expressed in embryonic kidney. So, it is likely that anti-Bid antibody A00730 will work for WB with embryonic kidney.
Boster Scientific Support
Answered: 2019-12-12
Question
Is a blocking peptide available for product anti-Bid antibody (A00730)?
Verified Customer
Asked: 2019-12-06
Answer
We do provide the blocking peptide for product anti-Bid antibody (A00730). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-06
Question
We bought anti-Bid antibody for WB on brain skin in the past. I am using rat, and We intend to use the antibody for IHC next. We want examining brain skin as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Asked: 2019-12-03
Answer
I looked at the website and datasheets of our anti-Bid antibody and I see that A00730 has been validated on rat in both WB and IHC. Thus A00730 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-03
Question
Can you help my question with product A00730, anti-Bid antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Asked: 2019-11-21
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00730 anti-Bid antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-21
Question
We are currently using anti-Bid antibody A00730 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Asked: 2019-10-08
Answer
The anti-Bid antibody (A00730) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-08
Question
My lab would like using your anti-Bid antibody for apoptotic mitochondrial changes studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Answer
I appreciate your inquiry. This A00730 anti-Bid antibody is tested on mouse spleen tissue, tissue lysate, rat spleen tissue, brain tissue. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-06-21
Question
Would A00730 anti-Bid antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Gonzalez
Asked: 2018-01-23
Answer
It shows on the product datasheet, A00730 anti-Bid antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-23
Question
Our team were satisfied with the WB result of your anti-Bid antibody. However we have been able to see positive staining in embryonic kidney isoform 3: cytoplasm using this antibody. Is that expected? Could you tell me where is BID supposed to be expressed?
B. Anderson
Asked: 2015-06-18
Answer
Based on literature, embryonic kidney does express BID. Generally BID expresses in cytoplasm., bh3-interacting domain death agonist p15:, bh3-interacting domain death agonist p13:, isoform 1: cytoplasm, isoform 3: cytoplasm, isoform 2: mitochondrion membrane. Regarding which tissues have BID expression, here are a few articles citing expression in various tissues:
Brain, and Skin, Pubmed ID: 15489334
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2015-06-18
Question
I am interested in to test anti-Bid antibody A00730 on mouse embryonic kidney for research purposes, then I may be interested in using anti-Bid antibody A00730 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
O. Zhang
Asked: 2013-03-25
Answer
The products we sell, including anti-Bid antibody A00730, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-03-25
