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- Table of Contents
METTL1 antibody (A10859-1) performs well in ELISA, IF, IHC, ICC, WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of METTL1 using anti-METTL1 antibody (A10859-1).
METTL1 was detected in paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL1 Antibody (A10859-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
METTL1 was detected in paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-METTL1 Antibody (A10859-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Anti-METTL1 Antibody Picoband®
METTL1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | ELISA, IF, IHC, ICC, WB |
Tested Applications
METTL1 antibody was positively detected by WB in:
human HELA whole cell, human Jurkat whole cell, human HEK293 whole cell, human A549 whole cell, human MCF-7 whole cell, human PC-3 whole cell, human T-47D whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
METTL1 antibody was positively detected by IHC in:
rat pancreas tissue, human breast cancer tissue, human gastric cancer tissue, human pancreatic cancer tissue, human skin cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
METTL1 antibody was positively detected by ICC/IF in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-METTL1 Antibody Picoband®
View all METTL1 Antibodies
View all METTL1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human METTL1 recombinant protein (Position: A2-H276).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
34 kDa
Calculated Molecular Weight
31.5 kDa
Background of METTL1
tRNA (guanine-N (7)-)-methyltransferase is an enzyme that in humans is encoded by the METTL1 gene. This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation. Alternative splice variants encoding different protein isoforms have been described for this gene. A pseudogene has been identified on chromosome X.
Cite This Product
Anti-METTL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10859-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Cow
92%
High Homology
Sheep
91%
High Homology
Dog
91%
High Homology
Rabbit
89%
Moderate Homology
Rat
88%
Moderate Homology
Mouse
88%
Moderate Homology
Zebrafish
66%
Low homology
Drosophila
55%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for METTL1 antibody
Immunohistochemistry (IHC) protocol for METTL1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for METTL1 antibody
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Product Promise
We promise Anti-METTL1 Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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A10859-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026