Product Info Summary
SKU: | M11817 |
---|---|
Size: | 100ug |
Reactive Species: | Mouse |
Host: | Mouse |
Application: | ELISA, WB |
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Product info
Product Name
Anti-BIN3 Monoclonal Antibody
SKU/Catalog Number
M11817
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-BIN3 Monoclonal Antibody (Catalog # M11817). Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-BIN3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11817)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: 3A4
Isotype
IgG1
Immunogen
Anti-BIN3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with a full length human BIN3 recombinant protein followed by hybridoma development.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M11817 is reactive to BIN3 in Mouse
Reconstitution
Calculated molecular weight
29665 MW
Background of BIN3
BIN-3 encodes an evolutionarily conserved and ubiquitously expressed member of the BAR superfamily of curved membrane and GTPase-binding proteins implicated in signal transduction and vesicular trafficking. In humans, Bin3 maps to chromosome 8p21.3, a region widely implicated in cancer suppression that is often deleted in non–Hodgkin’s lymphomas and various epithelial tumors. Study in mouse suggests that homozygous inactivation of Bin3 causes cataracts and an increased susceptibility to lymphomas during aging.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11817 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5000 - 1:50000
IP: 10-100 µL
WB: 1:500-1:1500
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of BIN3 expression in MEF lysate BIN3 +/-(lane 1) and MEF lysate BIN3 -/-(lane 2). BIN3 at 31KD was detected using mouse anti-BIN3 Antigen Affinity purified monoclonal antibody (Catalog # M11817) at 1:400. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
Click image to see more details
Western blot analysis of BIN3 expression in MEF cells wild type (lane 1) and MEF cells null mice (lane 2). BIN3 at 30KD was detected using mouse anti-BIN3 Antigen Affinity purified monoclonal antibody (Catalog # M11817). The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
Protein Target Info & Infographic
Gene/Protein Information For BIN3 (Source: Uniprot.org, NCBI)
Gene Name
BIN3
Full Name
Bridging integrator 3
Weight
29665 MW
Alternative Names
BIN3, Bridging integrator 3, BIN-3, BIN 3, BIN3, anti-BIN-3 BIN3 bridging integrator 3 bridging integrator 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BIN3, check out the BIN3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BIN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BIN3 Monoclonal Antibody (M11817)
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No publications found for M11817
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Customer Q&As
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6 Customer Q&As for Anti-BIN3 Monoclonal Antibody
Question
Would anti-BIN3 Monoclonal antibody M11817 work on pig WB with lung skin?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
Our lab technicians have not validated anti-BIN3 Monoclonal antibody M11817 on pig. You can run a BLAST between pig and the immunogen sequence of anti-BIN3 Monoclonal antibody M11817 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig lung skin in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-24
Question
Is this M11817 anti-BIN3 Monoclonal antibody reactive to the isotypes of BIN3?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
The immunogen of M11817 anti-BIN3 Monoclonal antibody is anti-BIN3 (MOUSE) Monoclonal antibody was produced in mouse by repeated immunizations with a full legnth human BIN3 recombinant protein followed by hybridoma development. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-22
Question
Will anti-BIN3 Monoclonal antibody M11817 work for IP with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2018-04-04
Answer
According to the expression profile of cervix carcinoma, BIN3 is highly expressed in cervix carcinoma. So, it is likely that anti-BIN3 Monoclonal antibody M11817 will work for IP with cervix carcinoma.
Boster Scientific Support
Answered: 2018-04-04
Question
We are currently using anti-BIN3 Monoclonal antibody M11817 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
M. Roberts
Verified customer
Asked: 2018-01-16
Answer
The anti-BIN3 Monoclonal antibody (M11817) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-16
Question
See attached the WB image, lot number and protocol we used for cervix carcinoma using anti-BIN3 Monoclonal antibody M11817. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-11-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-17
Question
I see that the anti-BIN3 Monoclonal antibody M11817 works with IP, what is the protocol used to produce the result images on the product page?
F. Zhao
Verified customer
Asked: 2016-10-25
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-10-25