Anti-Brd4 Rabbit Monoclonal Antibody

Boster Bio Anti-Brd4 Rabbit Monoclonal Antibody catalog # M00123. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00123
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

Product Name

Anti-Brd4 Rabbit Monoclonal Antibody

See all BRD4 products

SKU/Catalog Number

M00123

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Brd4 Rabbit Monoclonal Antibody catalog # M00123. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Brd4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00123)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DBH-2

Immunogen

A synthesized peptide derived from human Brd4

Reactive Species

M00123 is reactive to BRD4 in Human, Mouse, Rat

Applications

M00123 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For BRD4 (Source: Uniprot.Org, NCBI)

Uniprot ID

O60885

Gene ID

23476

Gene Name

BRD4

Full Name

Bromodomain-containing protein 4

Weight

152.219kDa

Alternative Names

BRD4; bromodomain containing 4; bromodomain-containing 4; CAPchromosome-associated protein; HUNK1; HUNK1bromodomain-containing protein 4; HUNKI; MCAP; Protein HUNK1 BRD4 CAP, HUNK1, HUNKI, MCAP bromodomain containing 4 bromodomain-containing protein 4|chromosome-associated protein|mitotic chromosome-associated protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on BRD4, check out the BRD4 Infographic

BRD4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for BRD4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for M00123

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7 Customer Q&As for Anti-Brd4 Rabbit Monoclonal Antibody

Question

Will anti-Brd4 Rabbit Monoclonal antibody M00123 work on feline IHC with liver?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

Our lab technicians have not validated anti-Brd4 Rabbit Monoclonal antibody M00123 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Brd4 Rabbit Monoclonal antibody M00123 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline liver in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-16

Question

Can you help my question with product M00123, anti-Brd4 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-02

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00123 anti-Brd4 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-02

Question

We are currently using anti-Brd4 Rabbit Monoclonal antibody M00123 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-18

Answer

The anti-Brd4 Rabbit Monoclonal antibody (M00123) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-18

Question

Would M00123 anti-Brd4 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-12-26

Answer

It shows on the product datasheet, M00123 anti-Brd4 Rabbit Monoclonal antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-12-26

Question

I was wanting to use your anti-Brd4 Rabbit Monoclonal antibody for ICC for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?

Verified Customer

Verified customer

Asked: 2018-01-25

Answer

You can see on the product datasheet, M00123 anti-Brd4 Rabbit Monoclonal antibody has been tested for IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-25

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Brd4 Rabbit Monoclonal antibody M00123. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-12-29

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-12-29

Question

Will anti-Brd4 Rabbit Monoclonal antibody M00123 work for ICC with cervix carcinoma erythroleukemia?

C. Jones

Verified customer

Asked: 2014-10-30

Answer

According to the expression profile of cervix carcinoma erythroleukemia, BRD4 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Brd4 Rabbit Monoclonal antibody M00123 will work for ICC with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2014-10-30

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
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M00123
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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