Anti-Zebrafish siah1 Antibody

Product Name

Anti-Zebrafish siah1 Antibody

View all Siah1 Antibodies

SKU/Catalog Number

DZ02095

Size

200 μl/vial

Form

Liquid

Description

Boster Bio Anti-siah1 Antibody catalog # DZ02095. This antibody reacts with Zebrafish.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Zebrafish siah1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ02095)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Clone Number

23N41

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence of human RAG1 (KHHWLYTSKYLQKFMNA).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

DZ02095 is reactive to SIAH1 in Zebrafish

Applications

DZ02095 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

16 kDa

Calculated molecular weight

31.123kDa

Background of Siah1

Recombination activating gene 1 also known as RAG-1 is a protein that in humans is encoded by the RAG1 gene. The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Mouse

Validation Images & Assay Conditions

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Figure 1. IF analysis of Siah1 using anti-Siah1 antibody (DZ02095).
Siah1 was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Siah1 Antibody (DZ02095) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®594 Conjugated Avidin (BA1142). Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For SIAH1 (Source: Uniprot.org, NCBI)

Alternative Names

E3 ubiquitin-protein ligase SIAH1; EC 6.3.2; EC 6.3.2.-; FLJ08065; hSIAH1; HUMSIAH; seven in absentia homolog 1 (Drosophila); Seven in absentia homolog 1; Siah-1; SIAH1A; siah-1a SIAH1 SIAH1A siah E3 ubiquitin protein ligase 1 E3 ubiquitin-protein ligase SIAH1|RING-type E3 ubiquitin transferase SIAH1|seven in absentia homolog 1|siah-1a

For more info on SIAH1, check out the SIAH1 Infographic

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In this infographic, you will see the following information for SIAH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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