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Product Info Summary
| SKU: | A01888-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Brm SMARCA2 Antibody
SKU/Catalog Number
A01888-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Brm SMARCA2 Antibody catalog # A01888-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Brm SMARCA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01888-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human Brm. AA range:1328-1377
Reactive Species
A01888-1 is reactive to SMARCA2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
181.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01888-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of extracts from A549 cells, using Brm Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from K562 cells, using Brm antibody.
Specific Publications For Anti-Brm SMARCA2 Antibody (A01888-1)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Brm SMARCA2 Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-Brm antibody A01888-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-13
Question
Does anti-Brm antibody A01888-1 work for WB with embryonic kidney?
G. Walker
Verified customer
Asked: 2020-02-13
Answer
According to the expression profile of embryonic kidney, SMARCA2 is highly expressed in embryonic kidney. So, it is likely that anti-Brm antibody A01888-1 will work for WB with embryonic kidney.
Boster Scientific Support
Answered: 2020-02-13
Question
I have attached the WB image, lot number and protocol we used for embryonic kidney using anti-Brm antibody A01888-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-03
Question
We need to test anti-Brm antibody A01888-1 on rat embryonic kidney for research purposes, then I may be interested in using anti-Brm antibody A01888-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The products we sell, including anti-Brm antibody A01888-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-12
Question
Will anti-Brm antibody A01888-1 work on monkey WB with liver?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
Our lab technicians have not tested anti-Brm antibody A01888-1 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Brm antibody A01888-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-17
Question
I was wanting to use your anti-Brm antibody for WB for rat embryonic kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat embryonic kidney identification?
K. Parker
Verified customer
Asked: 2015-01-02
Answer
You can see on the product datasheet, A01888-1 anti-Brm antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-01-02
Question
Will A01888-1 anti-Brm antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Carter
Verified customer
Asked: 2014-02-05
Answer
It shows on the product datasheet, A01888-1 anti-Brm antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-02-05
