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Product Info Summary
| SKU: | A00390 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-C-C chemokine receptor type 7 CCR7 Antibody
SKU/Catalog Number
A00390
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-C-C chemokine receptor type 7 CCR7 Antibody (Catalog # A00390). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
CCR7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-C-C chemokine receptor type 7 CCR7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00390)
Host
Rabbit
Contents
CCR7 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CCR7 antibody was raised against a 15 amino acid peptide near the center of human CCR7. The immunogen is located within amino acids 180 - 230 of CCR7.
Cross-reactivity
CCR7 antibody is human specific. CCR7 antibody is predicted to not cross-react with other CCR proteins.
Reactive Species
A00390 is reactive to CCR7 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
42.9 kDa
Background of CCR7
The CCR7 protein is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation (1,2). The chemokine (C-C motif) ligand 19 (CCL19) has been reported to be a specific ligand of this receptor (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00390 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CCR7 antibody can be used for detection of CCR7 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CCR7 in human spleen tissue lysate with CCR7 antibody at 1 μg/ml.
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Immunohistochemistry of CCR7 in human spleen tissue with CCR7 antibody at 5 μg/mL.
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Immunofluorescence of CCR7 in human spleen tissue with CCR7 antibody at 20 μg/mL.
Specific Publications For Anti-C-C chemokine receptor type 7 CCR7 Antibody (A00390)
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