Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CCL21/6Ckine. 96wells/kit, with removable strips. Cited in 1 publication(s).
Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine™
See all CCL21/6Ckine primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human 6Ckine/CCL21 with <12pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. 6Ckine/CCL21 tissue specificity: Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen.|
|Cite This Product||Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0555)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: S24-P134|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For CCL21 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||6366|
|Species Of This Entry||Human|
|Protein Name||C-C motif chemokine 21|
|Superfamily||intercrine beta (chemokine CC) family|
|Alternative Names||CCL21/6Ckine|6Ckine; 6CkineSmall-inducible cytokine A21; Beta-chemokine exodus-2; CCL21; chemokine (C-C motif) ligand 21; CKb9; exodus-2; member 21; SCYA21; SCYA21MGC34555; secondary lymphoid tissue chemokine; SLC; SLCSecondary lymphoid-tissue chemokine; TCA-4|
|Post Tranlational Modifications||Phosphorylation; Reduction; Cleavage; Glycosylation; Biotinylation; Deacetylation; Methylation|
|Gene Location||9p13.3, on Chromasome 9, gene sequence: NC_000009.12 (34709005..34710136, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CCL21 (Source: Uniprot.org, NCBI)
|Protein Function||Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.|
|Tissue Specificity||Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen.|
|Interacting Proteins||CCL13; CCL17; CCL26; CCL28; CCL5; CXCL10; CXCL12; CXCL14; CXCL9; PF4; PF4V1|
|Related Diseases||Neoplasms; Inflammation; Malignant Neoplasms; Tissue Adhesions; Cholangiocarcinoma; Neoplasm Metastasis; Autoimmune Reaction; Rheumatoid Arthritis; Arthritis; Carcinoma; Melanoma; Cell Invasion; Lymphatic Metastasis; Autoimmune Diseases; Malignant Neoplasm Of Breast; Chronic Inflammation; Metastatic Malignant Neoplasm To The Lymph Node; Malignant Paraganglionic Neoplasm; Infective Disorder; Mammary Neoplasms|
|Related Pathways||Chemotaxis; Cell Migration; Immune Response; Pathogenesis; Secretion; Localization; Cell Adhesion; Cell Proliferation; Lymphocyte Migration; Cell Activation; Cytokine Production; Dendritic Cell Migration; T Cell Migration; Hypersensitivity; T Cell Proliferation; Sensitization; T Cell Activation; Angiogenesis; Transport; Inflammatory Response|
|Background||Chemokine(C-C motif) ligand 21(CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine(because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine(SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13(CXCL13), CC chemokine ligand 21(CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.|
|Scientific References||PMID: 9235955 by Nagira M., et al. Molecular cloning of a novel human CC chemokine secondary lymphoid- tissue chemokine that is a potent chemoattractant for lymphocytes and mapped to chromosome 9p13.|
PMID: 9257816 by Hedrick J.A., et al. Identification and characterization of a novel beta chemokine containing six conserved cysteines.
Kit Components And Assay QC Data Details Of Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0555-CAP||96-well plate precoated with anti-Human CCL21/6Ckine antibody||1|
|EK0555-ST||lyophilized recombinant Human CCL21/6Ckine standard||10ng/tube|
|EK0555-DA||biotinylated anti-Human CCL21/6Ckine antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0555 Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 1
|Low-dose paclitaxel improves the therapeutic efficacy of recombinant adenovirus encoding CCL21 chemokine against murine cancer||PubMed ID 25230206|
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