Anti-Macrophage Inflammatory Protein 3 beta/Ccl19 Antibody Picoband™

Product Name

Anti-Macrophage Inflammatory Protein 3 beta/Ccl19 Antibody Picoband™

View all CCL19/MIP-3 beta Antibodies

SKU/Catalog Number

A01605-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Macrophage Inflammatory Protein 3 beta/Ccl19 Antibody Picoband™ catalog # A01605-1. Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Macrophage Inflammatory Protein 3 beta/Ccl19 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01605-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived rat Ccl19/MIP-3 beta recombinant protein (Position: G26-S108). Rat Ccl19/MIP-3 beta shares 79.5% and 89.2% amino acid (aa) sequence identity with human and mouse Ccl19/MIP-3 beta, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01605-1 is reactive to CCL19 in Mouse, Rat

Applications

A01605-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

20 kDa

Calculated molecular weight

10.993kDa

Background of CCL19/MIP-3 beta

Chemokine (C-C motif) ligand 19 (CCL19) is a protein that in humans is encoded by the CCL19 gene. This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Ccl19/MIP-3 beta using anti-Ccl19/MIP-3 beta antibody (A01605-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysate,
Lane 2: rat lung tissue lysate,
Lane 3: rat spleen tissue lysate,
Lane 4: rat stomach tissue lysate,
Lane 5: rat PC-12 whole Cell lysate,
Lane 6: mouse thymus tissue lysate,
Lane 7: mouse NIH3T3 whole Cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ccl19/MIP-3 beta antigen affinity purified polyclonal antibody (Catalog # A01605-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ccl19/MIP-3 beta at approximately 20KD. The expected band size for Ccl19/MIP-3 beta is at 11KD.

Gene/Protein Information For CCL19 (Source: Uniprot.org, NCBI)

Alternative Names

beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19 CCL19 CKb11, ELC, MIP-3b, MIP3B, SCYA19 C-C motif chemokine ligand 19 C-C motif chemokine 19|CC chemokine ligand 19|CK beta-11|EBI1-ligand chemokine|MIP-3-beta|beta chemokine exodus-3|chemokine (C-C motif) ligand 19|epstein-Barr virus-induced molecule 1 ligand chemokine|exodus-3|macrophage inflammatory protein 3-beta|small inducible cytokine subfamily A (Cys-Cys), member 19|small-inducible cytokine A19

For more info on CCL19, check out the CCL19 Infographic

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In this infographic, you will see the following information for CCL19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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