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Product Info Summary
| SKU: | A01605-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Macrophage Inflammatory Protein 3 beta/Ccl19 Antibody Picoband®
SKU/Catalog Number
A01605-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Macrophage Inflammatory Protein 3 beta/Ccl19 Antibody Picoband® catalog # A01605-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Macrophage Inflammatory Protein 3 beta/Ccl19 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01605-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat Ccl19/MIP-3 beta recombinant protein (Position: G26-S108). Rat Ccl19/MIP-3 beta shares 79.5% and 89.2% amino acid (aa) sequence identity with human and mouse Ccl19/MIP-3 beta, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01605-1 is reactive to Ccl19 in Mouse, Rat
Observed Molecular Weight
20 kDa
Background of Ccl19
Chemokine (C-C motif) ligand 19 (CCL19) is a protein that in humans is encoded by the CCL19 gene. This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01605-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: rat thymus tissue, rat lung tissue, rat spleen tissue, rat stomach tissue, rat PC-12 whole Cell, mouse thymus tissue, mouse NIH3T3 whole Cell
Validation Images & Assay Conditions
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Western blot analysis of Ccl19/MIP-3 beta using anti-Ccl19/MIP-3 beta antibody (A01605-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysate,
Lane 2: rat lung tissue lysate,
Lane 3: rat spleen tissue lysate,
Lane 4: rat stomach tissue lysate,
Lane 5: rat PC-12 whole Cell lysate,
Lane 6: mouse thymus tissue lysate,
Lane 7: mouse NIH3T3 whole Cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ccl19/MIP-3 beta antigen affinity purified polyclonal antibody (Catalog # A01605-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ccl19/MIP-3 beta at approximately 20KD. The expected band size for Ccl19/MIP-3 beta is at 11KD.
Specific Publications For Anti-Macrophage Inflammatory Protein 3 beta/Ccl19 Antibody Picoband® (A01605-1)
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1 Customer Q&As for Anti-Macrophage Inflammatory Protein 3 beta/Ccl19 Antibody Picoband®
Question
We are currently using anti-Macrophage Inflammatory Protein 3 beta/Ccl19 antibody A01605-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
The anti-Macrophage Inflammatory Protein 3 beta/Ccl19 antibody (A01605-1) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-13
