|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-c-Fos Antibody|
|Description||Rabbit IgG polyclonal antibody for Proto-oncogene c-Fos(FOS) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-c-Fos Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1318)|
|Replacement Item||This antibody may replace the following items: sc-13017-X|sc-130181|sc-166102-X|sc-166940|sc-166940-X|sc-171-X|sc-183-X|sc-22794|sc-22794-X|sc-253|sc-253-G|sc-253-X|sc-271243|sc-271243-X|sc-271657|sc-271657-X|sc-28213|sc-28213-X|sc-28310|sc-28310-X|sc-376148|sc-376148-X|sc-390035|sc-390035-X|sc-398595|sc-398595-X|sc-413|sc-413-X|sc-447|sc-447-X|sc-48-G|sc-48-X|sc-48424|sc-48868-X|sc-48869-X|sc-52|sc-52-G|sc-52-X|sc-604-X|sc-605-X|sc-69272-X|sc-69273-X|sc-7202|sc-7202-X|sc-7203-X|sc-8013-X|sc-8047|sc-8047-X|sc-81209|sc-81485 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human c-Fos(170-187aa DQLEDEKSALQTEIANLL), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Proto-oncogene c-Fos|
|Molecular Weight||40695 MW|
|Protein Function||Nuclear phosphoprotein which forms a tight but non- covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD- binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. .|
|Sequence Similarities||Belongs to the bZIP family. Fos subfamily.|
|Subcellular Localization||Nucleus. Endoplasmic reticulum. Cytoplasm, cytosol. In quiescent cells, present in very small amounts in the cytosol. Following induction of cell growth, first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum requires dephosphorylation at Tyr-10 and Tyr-30.|
|Alternative Names||Proto-oncogene c-Fos;Cellular oncogene fos;G0/G1 switch regulatory protein 7;FOS;G0S7;|
|Research Areas|||epigenetics and nuclear signaling|transcription|domain families|hlh / leucine zipper| epigenetics and nuclear signaling|cancer susceptibility|proto-oncogenes| cancer|oncoproteins/suppressors|oncoproteins|transcription factors||
Background for Proto-oncogene c-Fos
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-c-Fos Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-c-Fos Antibody Images
Click the images to enlarge.
Lane 1: HT1080 Cell Lysate
Lane 2: COLO320 Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,