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Product Info Summary
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Anti-NGF/NGF Beta Antibody Picoband™
Boster Bio Anti-NGF/NGF Beta Antibody Picoband™ catalog # A00341. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NGF/NGF Beta Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00341)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived human NGF/NGF Beta recombinant protein (Position: S122-A241). Human NGF/NGF Beta shares 92.4% amino acid (aa) sequence identity with rat NGF/NGF Beta.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00341 is reactive to NGF in Human
A00341 is guaranteed for ELISA, WB Boster Guarantee
Background of beta-NGF
Nerve growth factor is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. The nucleotide sequence of human and mouse beta-NGF are very similar. The beta-subunits of nerve growth factor (NGFB) have been assigned to mouse chromosome 3 and human chromosome 1p22. The human gene for the beta subunit of nerve growth factor is located on the proximal short arm of chromosome 1. A mutation in the nerve growth factor beta gene (NGFB) causes loss of pain perception.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NGF/NGF Beta using anti-NGF/NGF Beta antibody (A00341).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Recombinant Human NGF/NGF Beta Protein 0.5ng
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NGF/NGF Beta antigen affinity purified polyclonal antibody (Catalog # A00341) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NGF/NGF Beta at approximately 13KD. The expected band size for NGF/NGF Beta is at 13KD.
Gene/Protein Information For NGF (Source: Uniprot.Org, NCBI)
Beta-nerve growth factor
Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428 NGF Beta-NGF, HSAN5B, NGF nerve growth factor beta-nerve growth factor|nerve growth factor (beta polypeptide)|nerve growth factor, beta subunit|pro-nerve growth factor long*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on NGF, check out the NGF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NGF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A00341 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury
Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats
Associations between proinflammatory cytokines in the synovial fluid and radiographic grading and pain-related scores in 47 consecutive patients with osteoarthritis of the knee
Huang S, Zhang X, Xu L, Li Q, Zhao Q. Exp Ther Med. 2014 Oct;8(4):1237-1240. Epub 2014 Jul 21. Expression Of Nerve Growth Factor In The Prostate Of Male Rats In Response To Chronic Stress And Sympathetic Denervation.
Luo Q, Liu W, Chen J, Wang M, Zeng W, Chen Z, Cheng A. Neural Regen Res. 2012 Feb 5;7(4):302-7. Doi: 10.3969/J.Issn.1673-5374.2012.04.011. Nerve Growth Factor And Inducible Nitric Oxide Synthase Expression In The Mesencephalon And Diencephalon, As...
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