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- Table of Contents
MYC antibody (PB9092) performs well in IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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IF analysis of c-Myc using anti-c-Myc antibody (PB9092) and anti-Beta Tubulin antibody (M01857-3).
c-Myc was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-c-Myc Antibody (PB9092) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
c-Myc was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-c-Myc Antibody (PB9092) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-c-Myc Antibody Picoband®
MYC antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, ICC, WB |
Tested Applications
MYC antibody was positively detected by WB in:
human 293T whole cell, human Hela whole cell, human K562 whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MYC antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-c-Myc Antibody Picoband®
View all MYC Antibodies
View all MYC Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human c-Myc recombinant protein (Position: E257-A439). Human c-Myc shares 91% amino acid (aa) sequences identity with both mouse and rat c-Myc.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60-65 kDa
Calculated Molecular Weight
48.8 kDa
Background of MYC
C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. C-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol (2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.
Cite This Product
Anti-c-Myc Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9092)
Protocols
Western Blot protocol for MYC antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for MYC antibody
Publications
Application:
Species:
Expression and potential molecular mechanism of TOP2A in metastasis of non-small cell lung cancer
Published: 2024-05-28
Journal: Scientific Reports
Application: WB
Impact Factor: 3.80
Species: Human
PMID: 38806610
Feiyiliu Mixture sensitizes EGFRDel19/T790M/C797S mutant non-small cell lung cancer to osimertinib by attenuating the PRC1/Wnt/EGFR pathway
Published: 2023-01-19
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 5.60
Species: Mouse
PMID: 36744262
The novel protein C3orf43 accelerates hepatocyte proliferation
Published: 2017-09-16
Journal: CELLULAR & MOLECULAR BIOLOGY LETTERS
Application: WB
Impact Factor: 1.26
Species: Rat
PMID: 28932249
The novel protein C9orf116 promotes rat liver cell line BRL-3A proliferation
Published: 2017-07-27
Journal: PLoS One
Application: WB
Impact Factor: 2.81
Species: Human,Rat
PMID: 28749992
RPB5-mediating Protein Is Required for the Proliferation of Hepatocellular Carcinoma Cells *
Published: 2011-02-10
Journal: JOURNAL OF BIOLOGICAL CHEMISTRY
Application: WB,IHC
Impact Factor: 5.33
Species: Human,Mouse
PMID: 21310960
Let-7a microRNA functions as a potential tumor suppressor in human laryngeal cancer
Published: 2009-11-01
Journal: ONCOLOGY REPORTS
Application: WB,IHC
Impact Factor: 1.52
Species: Human
Positional and expressive alteration of prohibitin during the induced differentiation of human hepatocarcinoma SMMC-7721 cells
Published: 2008-08-28
Journal: WORLD JOURNAL OF GASTROENTEROLOGY
Application: IF
Impact Factor: 0.00
Species: Human
PMID: 18763282
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Product Promise
We promise Anti-c-Myc Antibody Picoband® performs excellently in relevant applications for IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9092
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026