Anti-c-Rel Antibody Picoband®

Western blot analysis of c-Rel using anti-c-Rel antibody (PB9741).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Rel antigen affinity purified polyclonal antibody (Catalog # PB9741) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Rel at approximately 69 kDa. The expected band size for c-Rel is at 65 kDa.

IHC analysis of c-Rel using anti-c-Rel antibody (PB9741).
c-Rel was detected in a paraffin-embedded section of mouse Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-c-Rel Antibody (PB9741) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

IL-25 inhibited LPS-induced translocation of c-Rel in STAT3-dependent manner in pulmonary macrophages. ( A ) The level of STAT3 phosphorylation was detected using Western blot. ( B ) Quantitative analysis of STAT3 phosphorylation in primary culture of pulmonary macrophages using ImageJ. Values are expressed in arbitrary units (a.u.). ( C - D ) Western blot was performed to measure the protein levels of c-Rel in the cytoplasm ( C ) and nucleus ( D ) of pulmonary macrophages. ( E - F ) Quantitative analysis of c-Rel expresion in cytoplasm ( E ) and nucleus ( F ) of pulmonary macrophages using ImageJ. ( G ) Representative images of c-Rel immunofluorescence staining in primary culture of macrophage from lung. Scale bar, 50 μm. ( H ) The protein levels of IL-12 A and IL-23 A of pulmonary macrophages was measured using Western blot. ( I - J ) Quantitative analysis of IL-12 A and IL-23 A in pulmonary macrophages using ImageJ. Values are expressed in arbitrary units (a.u.). ( K ) The graphical abstract of this study, which was drawn by Figdraw. The experiment was repeated 3 times independently, and the similar trend was obtained. One way ANOVA was used for statistical analysis (* P < 0.05; ** P < 0.01; *** P < 0.001)
Index in PubMed under a CC BY license. PMID: 37898756

IHC analysis of c-Rel using anti-c-Rel antibody (PB9741).
c-Rel was detected in a paraffin-embedded section of rat Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-c-Rel Antibody (PB9741) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

IHC analysis of c-Rel using anti-c-Rel antibody (PB9741).
c-Rel was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-c-Rel Antibody (PB9741) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.