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Product Info Summary
| SKU: | M05876 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Monkey |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-CALCOCO2 Mouse Monoclonal Antibody [Clone ID: OTI4H5]
SKU/Catalog Number
M05876
Size
100 µl
Description
Boster Bio CALCOCO2 mouse monoclonal antibody, clone OTI4H5 (formerly 4H5). Catalog# M05876. Tested in FC, IF, WB. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-CALCOCO2 Mouse Monoclonal Antibody [Clone ID: OTI4H5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05876)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI4H5
Isotype
IgG1
Immunogen
Full length human recombinant protein of human CALCOCO2 (NP_005822) produced in HEK293T cell.
Reactive Species
M05876 is reactive to CALCOCO2 in Human, Monkey
Observed Molecular Weight
52 kDa
Calculated molecular weight
52.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05876 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500~2000
IF: 1:100
Flow cytometry: 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CALCOCO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CALCOCO2.
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Figure from citation: Western Blot of CALCOCO2 (NDP52) protein level by using anti-CALCOCO2 antibody in human HEp-2 cell lysates.
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Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-CALCOCO2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CALCOCO2 monoclonal antibody.
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HEK293T cells transfected with either CALCOCO2 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CALCOCO2 antibody (M05876)
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Anti-CALCOCO2 mouse monoclonal antibody (M05876) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CALCOCO2.
Specific Publications For Anti-CALCOCO2 Mouse Monoclonal Antibody [Clone ID: OTI4H5] (M05876)
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