Anti-PU.1/Spi1 Antibody

Boster Bio Anti-PU.1/Spi1 Antibody catalog # RP1097. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: RP1097
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC

Product Name

Anti-PU.1/Spi1 Antibody

See all PU.1/Spi-1 products

SKU/Catalog Number

RP1097

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PU.1/Spi1 Antibody catalog # RP1097. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PU.1/Spi1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1097)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human PU.1/Spi1 recombinant protein (Position: E18-K196). Human PU.1/Spi1 shares 84% and 84.4% amino acid (aa) sequence identity with mouse and rat PU.1/Spi1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1097 is reactive to SPI1 in Human, Mouse, Rat

Applications

RP1097 is guaranteed for IHC Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat

Validation Images & Assay Conditions

Gene/Protein Information For SPI1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P17947

Gene ID

6688

Gene Name

SPI1

Full Name

Transcription factor PU.1

Weight

31.083kDa

Superfamily

ETS family

Alternative Names

hematopoietic transcription factor PU.1; OF; PU.1; SFPI1; SFPI131 kDa transforming protein; SPI-1 proto-oncogene; SPI1; Spi-1; SPI-A; spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein; transcription factor PU.1 SPI1 OF, PU.1, SFPI1, SPI-1, SPI-A Spi-1 proto-oncogene transcription factor PU.1|31 kDa transforming protein|hematopoietic transcription factor PU.1|spleen focus forming virus (SFFV) proviral integration oncogene spi1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SPI1, check out the SPI1 Infographic

SPI1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SPI1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for RP1097

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1 Customer Q&As for Anti-PU.1/Spi1 Antibody

Question

We are currently using anti-PU.1/Spi1 antibody RP1097 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?

S. Collins

Verified customer

Asked: 2014-06-16

Answer

The anti-PU.1/Spi1 antibody (RP1097) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-06-16

Conjugation

Size

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Sample Inquiry

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RP1097
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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