Anti-PU.1/Spi1 Antibody

Product Name

Anti-PU.1/Spi1 Antibody

View all SPI1 Antibodies

SKU/Catalog Number

RP1097
PB0785 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PU.1/Spi1 Antibody catalog # RP1097. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PU.1/Spi1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1097)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PU.1/Spi1 recombinant protein (Position: E18-K196). Human PU.1/Spi1 shares 84% and 84.4% amino acid (aa) sequence identity with mouse and rat PU.1/Spi1, respectively.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1097 is reactive to SPI1 in Human, Mouse, Rat

Calculated molecular weight

31.1 kDa

Background of SPI1

Transcription factor PU.1 is a protein that in humans is encoded by the SPI1 gene. This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

RP1097 is guaranteed for IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat

Positive Control

IHC: Mouse Spleen Tissue, Rat Spleen Tissue, Human Tonsil Tissue

Validation Images & Assay Conditions

See full target information SPI1

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