Click image to see more details
Product Info Summary
| SKU: | A03135-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-ARHGDIA/Rhogdi Antibody
SKU/Catalog Number
A03135-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ARHGDIA/Rhogdi Antibody (Catalog# A03135-1). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ARHGDIA/Rhogdi Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03135-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human ARHGDIA. Immunogen sequence location: 140-189
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03135-1 is reactive to ARHGDIA in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
23.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03135-1 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ELISA: 1:10000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue, using ARHGDIA Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-ARHGDIA/Rhogdi Antibody (A03135-1)
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Customer Q&As
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7 Customer Q&As for Anti-ARHGDIA/Rhogdi Antibody
Question
Will anti-ARHGDIA/Rhogdi antibody A03135-1 work for IHC with skin?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
According to the expression profile of skin, ARHGDIA is highly expressed in skin. So, it is likely that anti-ARHGDIA/Rhogdi antibody A03135-1 will work for IHC with skin.
Boster Scientific Support
Answered: 2019-12-23
Question
I see that the anti-ARHGDIA/Rhogdi antibody A03135-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-01
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-ARHGDIA/Rhogdi antibody A03135-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-08
Question
We are currently using anti-ARHGDIA/Rhogdi antibody A03135-1 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
The anti-ARHGDIA/Rhogdi antibody (A03135-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-26
Question
We need to test anti-ARHGDIA/Rhogdi antibody A03135-1 on mouse skin for research purposes, then I may be interested in using anti-ARHGDIA/Rhogdi antibody A03135-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The products we sell, including anti-ARHGDIA/Rhogdi antibody A03135-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-28
Question
Does A03135-1 anti-ARHGDIA/Rhogdi antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
As indicated on the product datasheet, A03135-1 anti-ARHGDIA/Rhogdi antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-11
Question
I have a question about product A03135-1, anti-ARHGDIA/Rhogdi antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Patel
Verified customer
Asked: 2018-03-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03135-1 anti-ARHGDIA/Rhogdi antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-05
