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Product Info Summary
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Anti-ARID5A Antibody Picoband™
Boster Bio Anti-ARID5A Antibody Picoband™ catalog # A14678-1. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ARID5A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14678-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
E.coli-derived human ARID5A recombinant protein (Position: R55-L594).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
A14678-1 is reactive to ARID5A in Human
A14678-1 is guaranteed for ELISA, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ARID5A using anti-ARID5A antibody (A14678-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARID5A antigen affinity purified polyclonal antibody (Catalog # A14678-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARID5A at approximately 64KD. The expected band size for ARID5A is at 64KD.
Gene/Protein Information For ARID5A (Source: Uniprot.Org, NCBI)
AT-rich interactive domain-containing protein 5A
ARID domain-containing protein 5A; AT rich interactive domain 5A (MRF1-like); AT-rich interactive domain-containing protein 5A; RFVG5814 ARID5A MRF-1, MRF1, RP11-363D14 AT-rich interaction domain 5A AT-rich interactive domain-containing protein 5A|ARID domain-containing protein 5A|AT rich interactive domain 5A (MRF1-like)|RFVG5814|modulator recognition factor 1|modulator recognition factor I*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ARID5A, check out the ARID5A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ARID5A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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