This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Ctsd antibody (PB9048) performs well in Flow Cytometry, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
Flow Cytometry analysis of mouse liver single-cell suspension using anti-Cathepsin D/CTSD antibody (PB9048).
Overlay histogram showing mouse liver single-cell suspension stained with Cathepsin D/CTSD (Blue line). mouse liver single-cell suspension had been fixed and permeabilized. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cathepsin D/CTSD Antibody (PB9048, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing mouse liver single-cell suspension stained with Cathepsin D/CTSD (Blue line). mouse liver single-cell suspension had been fixed and permeabilized. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cathepsin D/CTSD Antibody (PB9048, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Bundle Save
Anti-Cathepsin D/CTSD Antibody Picoband®
Ctsd antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | Flow Cytometry, IHC, WB |
Tested Applications
Ctsd antibody was positively detected by WB in:
Mouse Liver Tissue, Mouse Brain Tissue, Mouse Thymus Tissue, NEURO Whole Cell,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Ctsd antibody was positively detected by IHC in:
Mouse Kidney tissue, Mouse Liver tissue, rat liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Ctsd antibody was positively detected by FC in:
mouse liver tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Mouse
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
FC
Mouse
Tested
Tested
Recommend
Tested
Rat
Tested
Recommend
Product Information
Product Name
Anti-Cathepsin D/CTSD Antibody Picoband®
View all CTSD Antibodies
View all CTSD Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Cathepsin D recombinant protein (Position: E65-L410). Mouse Cathepsin D shares 85% and 91% amino acid (aa) sequences identity with human and rat Cathepsin D, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated Molecular Weight
45.0 kDa
Background of Ctsd
Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
Cite This Product
Anti-Cathepsin D/CTSD Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9048)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
92%
High Homology
Monkey
86%
Moderate Homology
Human
85%
Moderate Homology
Dog
85%
Moderate Homology
Sheep
83%
Moderate Homology
Cow
82%
Moderate Homology
Zebrafish
70%
Moderate Homology
Drosophila
58%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Ctsd antibody
Immunohistochemistry (IHC) protocol for Ctsd antibody
Flow Cytometry (FC) protocol for Ctsd antibody
Publications
Application:
Species:
Dynamics of neurodegeneration in the hippocampus of Krushinsky-Molodkina rats correlates with the progression of limbic seizures
Published: 2022-07-16
Journal: EPILEPSY & BEHAVIOR
Application: IF
Impact Factor: 3.34
Species: Rat
PMID: 35849865
Review
Have you used Anti-Cathepsin D/CTSD Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-Cathepsin D/CTSD Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9048
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026